Rabbit anti-Mouse Suppressors Of Cytokine Signaling 3 (SOCS3) Polyclonal Antibody | anti-SOCS3 antibody

Polyclonal Antibody to Suppressors Of Cytokine Signaling 3 (SOCS3)

Cat. Num. AAA2001901

• Gene Names: Socs3; Cis3; Ef10; Ssi3; Cish3; EF-10; SSI-3
• Reactivity: Mouse
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Suppressors Of Cytokine Signaling 3 (SOCS3); Polyclonal Antibody; Polyclonal Antibody to Suppressors Of Cytokine Signaling 3 (SOCS3); anti-SOCS3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against SOCS3. It has been selected for its ability to recognize SOCS3 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with a His-tag and its sequence is listed below.; MGHHHHHHSGSEF-MVTHSKFPAA GMSRPLDTSL RLKTFSSKSE YQLVVNAVRK LQESGFYWSA VTGGEANLLL SAEPAGTFLI RDSSDQRHFF TLSVKTQSGT KNLRIQCEGG SFSLQSDPRS TQPVPRFDCVLKLVHHYMPP PGTPSFSLPP TEPSSEVPEQ PPAQALPGST PKRAYYIYSG GEKIPLVLSR PLSSNVATLQ HLCRKTVNGH LDSYEKVTQL PGPIREFLDQ YDAPL
• Sequence Length: 225

• Applicable Applications for anti-SOCS3 antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant SOCS3 (Met1~Leu225) expressed in E.coli.
• Cross Reactivity: Mouse
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2057867
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse SOCS3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded Spleen tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 6671758
• NCBI GeneID: 12702
• NCBI Accession #: NP_031733.1
• NCBI GenBank Nucleotide #: NM_007707.3
• UniProt Accession #: O35718; P97803; Q3U7X5
• Molecular Weight: 24,776 Da
• NCBI Official Full Name: suppressor of cytokine signaling 3
• NCBI Official Synonym Full Names: suppressor of cytokine signaling 3
• NCBI Official Symbol: Socs3
• NCBI Official Synonym Symbols: Cis3; Ef10; Ssi3; Cish3; EF-10; SSI-3
• NCBI Protein Information: suppressor of cytokine signaling 3
• UniProt Protein Name: Suppressor of cytokine signaling 3
• Protein Family: Suppressor of cytokine signaling
• UniProt Gene Name: Socs3
• UniProt Synonym Gene Names: Cis3; Cish3; SOCS-3; CIS-3

Uniprot Description

SOCS3: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo. Probable substrate recognition component of a SCF-like ECS (Elongin BC- CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST. Interacts with multiple activated proteins of the tyrosine kinase signaling pathway including IGF1 receptor, insulin receptor and JAK2. Binding to JAK2 is mediated through the KIR and SH2 domains to a phosphorylated tyrosine residue within the JAK2 JH1 domain. Binds specific activated tyrosine residues of the leptin, EPO, IL12, GSCF and gp130 receptors. Interaction with CSNK1E stabilizes SOCS3 protein. Component of the probable ECS(SOCS3) E3 ubiquitin-protein ligase complex which contains CUL5, RNF7/RBX2, Elongin BC complex and SOCS3. Interacts with CUL5, RNF7, TCEB1 and TCEB2. Interacts with CUL2. Interacts with FGFR3. Interacts with INSR. Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 11|11 E2

Cellular Component: cytoplasm

Molecular Function: phosphotyrosine binding; protein binding; protein kinase inhibitor activity

Biological Process: cytokine-mediated signaling pathway; JAK-STAT cascade; negative regulation of apoptosis; negative regulation of inflammatory response; negative regulation of insulin receptor signaling pathway; negative regulation of JAK-STAT cascade; negative regulation of protein kinase activity; negative regulation of signal transduction; negative regulation of tyrosine phosphorylation of STAT protein; positive regulation of cell differentiation; regulation of cell differentiation; regulation of growth; regulation of protein amino acid phosphorylation; signal transduction