Knockout Validation
(Knockout Validation: Lane 1: Wild-type MCF7 cell lysate; Lane 2: SOD2 knockout MCF7 cell lysate; Predicted MW: 18,20,21,25kd Observed MW: 21kd Primary Ab: 3ug/ml Rabbit Anti-Human SOD2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Rabbit anti-Human Superoxide Dismutase 2, Mitochondrial (SOD2) Polyclonal Antibody | anti-SOD2 antibody
Biotin-Linked Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2)
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Knockout Validation
(Knockout Validation: Lane 1: Wild-type MCF7 cell lysate; Lane 2: SOD2 knockout MCF7 cell lysate; Predicted MW: 18,20,21,25kd Observed MW: 21kd Primary Ab: 3ug/ml Rabbit Anti-Human SOD2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type MCF7 cell lysate; Lane 2: SOD2 knockout MCF7 cell lysate; Predicted MW: 18,20,21,25kd Observed MW: 21kd Primary Ab: 3ug/ml Rabbit Anti-Human SOD2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Uniprot Description
SOD2: Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the iron/manganese superoxide dismutase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.15.1.1; Oxidoreductase; Mitochondrial
Chromosomal Location of Human Ortholog: 6q25.3
Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane
Molecular Function: identical protein binding; DNA binding; manganese ion binding; superoxide dismutase activity; oxygen binding
Biological Process: oxygen homeostasis; positive regulation of nitric oxide biosynthetic process; removal of superoxide radicals; heart development; response to lipopolysaccharide; locomotory behavior; response to L-ascorbic acid; protein homotetramerization; post-embryonic development; negative regulation of cell proliferation; response to selenium ion; glutathione metabolic process; acetylcholine vasodilation involved in regulation of systemic arterial blood pressure; regulation of mitochondrial membrane potential; regulation of catalytic activity; regulation of blood pressure; response to gamma radiation; hemopoiesis; response to axon injury; negative regulation of neuron apoptosis; response to electrical stimulus; response to drug; erythrophore differentiation; response to superoxide; release of cytochrome c from mitochondria; superoxide metabolic process; negative regulation of fat cell differentiation; liver development; regulation of transcription from RNA polymerase II promoter; response to manganese ion; response to reactive oxygen species; iron ion homeostasis; response to silicon dioxide; response to cadmium ion; response to hyperoxia; response to hydrogen peroxide; DNA damage response, signal transduction resulting in induction of apoptosis; negative regulation of fibroblast proliferation; age-dependent response to reactive oxygen species; detection of oxygen; response to zinc ion; response to hypoxia; neuron development; response to cold; response to activity; induction of apoptosis by oxidative stress; superoxide release; hydrogen peroxide biosynthetic process
Disease: Microvascular Complications Of Diabetes, Susceptibility To, 6
Research Articles on SOD2
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Product Notes
The SOD2 sod2 (Catalog #AAA2003502) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Biotin-Linked Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Superoxide Dismutase 2, Mitochondrial (SOD2) can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the SOD2 sod2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Superoxide Dismutase 2, Mitochondrial (SOD2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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