Western Blot (WB)
(Anti-SOD1 antibody, MBS175666, Western blottingLane 1: COLO320 Cell LysateLane 2: SMMC Cell Lysate )
Rabbit Superoxide Dismutase 1 Polyclonal Antibody | anti-SOD1 antibody
Anti-Superoxide Dismutase 1 antibody
IHC-P: Concentration: 0.5-1ug/ml; Tested Species: Human, Mouse, Rat; Antigen Retrieval: By Heat
IHC-F: Concentration: 0.5-1ug/ml; Tested Species: Human; Predicted Species: Mouse, Rat
ICC: 0.5-1.0ug/ml; Tested Species: Human
Tested Species: In-house tested species with positives results.
Predicted Species: Species predicted to be fit for the product based on sequence similarties.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Western Blot (WB)
(Anti-SOD1 antibody, MBS175666, Western blottingLane 1: COLO320 Cell LysateLane 2: SMMC Cell Lysate )
Western Blot (WB)
(Anti-SOD1 antibody, MBS175666, Western blottingLane 1: COLO320 Cell LysateLane 2: SMMC Cell Lysate )
Immunohistochemistry (IHC)
(Anti-SOD1 antibody, MBS175666, IHC(P)IHC(P): Human Mammary Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-SOD1 antibody, MBS175666, IHC(P)IHC(P): Human Mammary Cancer Tissue )
Background: Superoxide dismutases(SOD) are a class of enzymes that catalyze the dismutation of superoxide into oxygen and hydrogen peroxide. As such, they are an important antioxidant defense in nearly all cells exposed to oxygen. One of the exceedingly rare exceptions is Lactobacillus plantarum and related lactobacilli, which use a different mechanism.Cu,Zn-SOD was found widely distributed in the cell cytosol and in the cell nucleus, consistent with it being a soluble cytosolic protein. Mitochondria and secretory compartments did not label for this protein. In human cells, peroxisomes showed a labeling density slightly less than that of cytoplasm.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
SOD1: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. Homodimer; non-disulfide linked. Homodimerization may take place via the ditryptophan cross-link at Trp-33. The pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 interact with RNF19A, whereas wild-type protein does not. The pathogenic variants ALS1 Arg-86 and Ala-94 interact with MARCH5, whereas wild-type protein does not. Belongs to the Cu-Zn superoxide dismutase family.
Protein type: Oxidoreductase; EC 1.15.1.1; Mitochondrial; Apoptosis; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 21q22.11
Cellular Component: dendrite cytoplasm; extracellular space; protein complex; mitochondrion; extracellular region; mitochondrial intermembrane space; cytosol; nucleoplasm; extracellular matrix; cell soma; mitochondrial matrix; cytoplasm; plasma membrane; peroxisome; cytoplasmic vesicle; nucleus
Molecular Function: identical protein binding; protein binding; protein homodimerization activity; copper ion binding; zinc ion binding; chaperone binding; superoxide dismutase activity; Rac GTPase binding; protein phosphatase 2B binding
Biological Process: positive regulation of catalytic activity; cellular iron ion homeostasis; positive regulation of apoptosis; activation of MAPK activity; myeloid cell homeostasis; retrograde axon cargo transport; response to antibiotic; muscle maintenance; retinal homeostasis; glutathione metabolic process; neurofilament cytoskeleton organization and biogenesis; regulation of mitochondrial membrane potential; positive regulation of superoxide release; negative regulation of neuron apoptosis; placenta development; positive regulation of cytokine production; response to drug; platelet activation; cell aging; transmission of nerve impulse; regulation of organ growth; response to reactive oxygen species; response to ethanol; heart contraction; response to heat; superoxide release; relaxation of vascular smooth muscle; removal of superoxide radicals; locomotory behavior; response to organic substance; sensory perception of sound; platelet degranulation; ovarian follicle development; regulation of blood pressure; response to axon injury; auditory receptor cell stereocilium organization and biogenesis; negative regulation of cholesterol biosynthetic process; anterograde axon cargo transport; response to nutrient levels; response to superoxide; thymus development; regulation of T cell differentiation in the thymus; response to amphetamine; superoxide metabolic process; myelin maintenance in the peripheral nervous system; regulation of multicellular organism growth; response to copper ion; response to hydrogen peroxide; spermatogenesis; regulation of protein kinase activity; blood coagulation; hydrogen peroxide biosynthetic process; embryo implantation
Disease: Amyotrophic Lateral Sclerosis 1
Research Articles on SOD1
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Product Notes
The SOD1 sod1 (Catalog #AAA175666) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Superoxide Dismutase 1 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Superoxide Dismutase 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry ICC. WB: Concentration: 0.1-0.5ug/ml; Tested Species: Human, Mouse, Rat IHC-P: Concentration: 0.5-1ug/ml; Tested Species: Human, Mouse, Rat; Antigen Retrieval: By Heat IHC-F: Concentration: 0.5-1ug/ml; Tested Species: Human; Predicted Species: Mouse, Rat ICC: 0.5-1.0ug/ml; Tested Species: Human Tested Species: In-house tested species with positives results. Predicted Species: Species predicted to be fit for the product based on sequence similarties. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the SOD1 sod1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Superoxide Dismutase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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