Western Blot (WB)
(Western Blot analysis of SUN2 on rat kidney tissue lysate using anti-SUN2 antibody at 1:2000 dilution.)
Rabbit SUN2 Polyclonal Antibody | anti-SUN2 antibody
SUN2 Polyclonal Antibody
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
ICC: 1:50-1:100
IF: 1:50-1:100
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of SUN2 on rat kidney tissue lysate using anti-SUN2 antibody at 1:2000 dilution.)
Western Blot (WB)
(Western Blot analysis of SUN2 on rat kidney tissue lysate using anti-SUN2 antibody at 1:2000 dilution.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-SUN2 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-SUN2 antibody. Counter stained with hematoxylin.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of SK-Br-3 cells with SUN2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of SK-Br-3 cells with SUN2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Immunocytochemistry (ICC)
(ICC staining SUN2 (green) in SK-Br-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining SUN2 (green) in SK-Br-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-SUN2 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-SUN2 antibody. Counter stained with hematoxylin.)
NCBI and Uniprot Product Information
NCBI Description
SUN1 (MIM 607723) and SUN2 are inner nuclear membrane (INM) proteins that play a major role in nuclear-cytoplasmic connection by formation of a 'bridge' across the nuclear envelope, known as the LINC complex, via interaction with the conserved luminal KASH domain of nesprins (e.g., SYNE1; MIM 608441) located in the outer nuclear membrane (ONM). The LINC complex provides a direct connection between the nuclear lamina and the cytoskeleton, which contributes to nuclear positioning and cellular rigidity (summary by Haque et al., 2010 [PubMed 19933576]).[supplied by OMIM, Nov 2010]
Uniprot Description
SUN2: a nuclear transmembrane protein. Forms part of a membrane-spanning fibrillar complex that interconnects attached meiotic telomeres with cytoplasmic structures. Together with SUN1 connects the nuclear envelope with the centrosome. Interacts with RAB5A. Reportedly expressed in the endosome where it may play a role in endosome fusion and endocytosis.
Protein type: Membrane protein, integral; Nuclear envelope
Chromosomal Location of Human Ortholog: 22q13.1
Cellular Component: nuclear membrane; condensed nuclear chromosome; nuclear chromosome, telomeric region; endosome membrane; integral to nuclear inner membrane; nuclear envelope
Molecular Function: identical protein binding; protein binding; microtubule binding; lamin binding
Biological Process: mitotic spindle organization and biogenesis; centrosome localization; nuclear migration along microfilament; nuclear migration; nuclear membrane organization and biogenesis; positive regulation of cell migration
Research Articles on SUN2
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Product Notes
The SUN2 sun2 (Catalog #AAA3014173) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SUN2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SUN2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 IHC: 1:50-1:200 FC/FACS: 1:50-1:100 ICC: 1:50-1:100 IF: 1:50-1:100. Researchers should empirically determine the suitability of the SUN2 sun2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SUN2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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