Rabbit anti-Human SUMO3 Polyclonal Antibody | anti-SUMO3 antibody

SUMO3, CT (Small Ubiquitin-Related Modifier 3, SUMO-3, Ubiquitin-Like Protein SMT3A, SMT3 Homolog 1, SUMO-2, SMT3A, SMT3H1)

Cat. Num. AAA645864

• Gene Names: SUMO3; SMT3A; Smt3B; SMT3H1; SUMO-3
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: SUMO3; Polyclonal Antibody; CT (Small Ubiquitin-Related Modifier 3; SUMO-3; Ubiquitin-Like Protein SMT3A; SMT3 Homolog 1; SUMO-2; SMT3A; SMT3H1); Anti -SUMO3; anti-SUMO3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human SUMO3. Species sequence homology: mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-SUMO3 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1-2ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to 13aa from human SUMO3 at C-terminus.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot analysis of SUMO3 in mouse liver tissue lysate with SUMO3 antibody at (A) and (B) 2ug/ml.)

Related Product Information for anti-SUMO3 antibody

Covalent modification of target lysines by SUMO (small ubiquitin-like modifier) modulates processes such as protein localization, transcription, nuclear transport, mitosis, DNA replication and repair, signal transduction, and viral reproduction. SUMO does not seem to be involved in protein degradation and may in fact function as an antagonist of ubiquitin in the degradation process. The SUMO family consists of SUMO1 and closely related homologs SUMO2, SUMO3, and SUMO4. Sumoylation has been shown to regulate a wide range of proteins, including MDM2, PIAS, PML, RanGAP1, RanBP2, p53, p73, HIPK2, TEL, c-Jun, Fas, Daxx, TNFRI, Topo-I, Topo-II, PARK2, WRN, Sp100, IkB-alpha, Androgen receptor (AR), GLUT1/4, CaMK, DNMT3B, TDG, HIF1A, CHD3, EXOSC9, RAD51, and viral targets such as CMV-IE1/2, EBV-BZLF1, and HPV/BPV-E1.

Product Categories/Family for anti-SUMO3 antibody

Antibodies; Abs to Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 48928058
• NCBI GeneID: 6612
• NCBI Accession #: NP_008867.2
• NCBI GenBank Nucleotide #: NM_006936.2
• UniProt Accession #: P55854; Q53HI9; Q6FGD4; Q9BWR4; B2R5X4
• Molecular Weight: 11,637 Da
• NCBI Official Full Name: small ubiquitin-related modifier 3
• NCBI Official Synonym Full Names: small ubiquitin-like modifier 3
• NCBI Official Symbol: SUMO3
• NCBI Official Synonym Symbols: SMT3A; Smt3B; SMT3H1; SUMO-3
• NCBI Protein Information: small ubiquitin-related modifier 3; SUMO-2; SMT3 homolog 1; ubiquitin-like protein SMT3A; ubiquitin-like protein SMT3B; SMT3 suppressor of mif two 3 homolog 1; SMT3 suppressor of mif two 3 homolog 3
• UniProt Protein Name: Small ubiquitin-related modifier 3
• Protein Family: Small ubiquitin-related modifier
• UniProt Gene Name: SUMO3
• UniProt Synonym Gene Names: SMT3B; SMT3H1; SUMO-3; Smt3B
• UniProt Entry Name: SUMO3_HUMAN

NCBI Description

SUMO proteins, such as SUMO3, and ubiquitin (see MIM 191339) posttranslationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability (Su and Li, 2002 [PubMed 12383504]).[supplied by OMIM, Mar 2008]

Uniprot Description

Function: Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. Ref.9 Ref.15

Subunit structure: Covalently attached to a number of proteins

By similarity. Interacts with USP25 (via ts SIM domain); the interaction sumoylates USP25 and inhibits its ubiquitin hydrolyzing activity. Interacts with SAE2 and UBE2I. Ref.9 Ref.11 Ref.15

Subcellular location: Cytoplasm Ref.10.

Tissue specificity: Expressed predominantly in liver. Ref.13

Post-translational modification: Polymeric chains can be formed through Lys-11 cross-linking.Cleavage of precursor form by SENP1, SENP2 or SENP5 is necessary for function.

Sequence similarities: Belongs to the ubiquitin family. SUMO subfamily.Contains 1 ubiquitin-like domain.

Caution: Frequently wrongly assigned as SMT3A in many databases and publications. However, according to initial nomenclature, SUMO3 corresponds to SMT3B (Ref.8).