Rabbit anti-Xenopus SUMO2 Polyclonal Antibody | anti-SUMO2 antibody

SUMO2, Xenopus, NT (sumo2-a, smt3h2, Small ubiquitin-related modifier 2-A) (Azide free) (HRP)

Cat. Num. AAA6352603

• Gene Names: sumo2.L; hsmt3; smt3b; sumo2; smt3h2; sumo-2; sumo2-a; sumo2-b
• Reactivity: Xenopus
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SUMO2; Polyclonal Antibody; Xenopus; NT (sumo2-a; smt3h2; Small ubiquitin-related modifier 2-A) (Azide free) (HRP); sumo2-a; Small ubiquitin-related modifier 2-A; anti-SUMO2 antibody

• Host: Rabbit
• Reactivity: Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SUMO2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Xenopus SUMO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of Xenopus SUMO2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SUMO2 (arrow) using rabbit polyclonal SUMO2 Antibody (M1) (Xenopus). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SUMO2 gene.)

Related Product Information for anti-SUMO2 antibody

SUMO2 is a ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. SUMO2 does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. This protein plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS isoforms 1-4.

Product Categories/Family for anti-SUMO2 antibody

Antibodies; Ubiquitin

References

Muller S, et al., Nat Rev Mol Cell Biol. 2001 2(3):202-10 Review. Hochstrasser M. Cell. 2001 107(1):5-8. Review. Kahyo T, et al., Mol Cell. 2001 Sep;8(3):713-8. Yeh ET, et al., Gene. 2000 May 2;248(1-2):1-14. Review. Keane, M.M., et al., Oncogene 18 (22), 3365-3375 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 147906503
• NCBI GeneID: 379777
• NCBI Accession #: NP_001080085.1
• NCBI GenBank Nucleotide #: NM_001086616.1
• UniProt Accession #: Q7ZTK7
• Molecular Weight: 10,820 Da
• NCBI Official Full Name: small ubiquitin-related modifier 2-A
• NCBI Official Synonym Full Names: small ubiquitin-like modifier 2 L homeolog
• NCBI Official Symbol: sumo2.L
• NCBI Official Synonym Symbols: hsmt3; smt3b; sumo2; smt3h2; sumo-2; sumo2-a; sumo2-b
• NCBI Protein Information: small ubiquitin-related modifier 2-A
• UniProt Protein Name: Small ubiquitin-related modifier 2-A
• Protein Family: Small ubiquitin-related modifier
• UniProt Gene Name: sumo2-a
• UniProt Synonym Gene Names: smt3h2; SUMO-2-A

Uniprot Description

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex sae1-sae2 and linkage to the E2 enzyme ube2i, and can be promoted by an E3 ligase such as pias1-4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric sumo2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins.