Rabbit anti-Human SUMO Polyclonal Antibody | anti-SUMO antibody

SUMO, Pan (Small Ubiquitin-related Modifier 1, SUMO-1, Ubiquitin-like Protein SMT3C, SMT3 Homolog 3, Ubiquitin-homology Domain Protein PIC1, Ubiquitin-like Protein UBL1, GAP Modifying Protein 1, GMP1, Sentrin, SMT3C, SMT3H3, UBL1, Small Ubiquitin-related

Cat. Num. AAA6501563

• Gene Names: SUMO1; DAP1; GMP1; PIC1; SMT3; UBL1; OFC10; SENP2; SMT3C; SMT3H3
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: SUMO; Polyclonal Antibody; Pan (Small Ubiquitin-related Modifier 1; SUMO-1; Ubiquitin-like Protein SMT3C; SMT3 Homolog 3; Ubiquitin-homology Domain Protein PIC1; Ubiquitin-like Protein UBL1; GAP Modifying Protein 1; GMP1; Sentrin; SMT3C; SMT3H3; UBL1; Small Ubiquitin-related; anti-SUMO antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes all 3 SUMO isoforms, including human SUMO1, SUMO2 and SUMO3.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 101

• Applicable Applications for anti-SUMO antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein encoding full length of human SUMO1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(The Pan SUMO Antibody is used in Western blot to detect SUMO1 in GST(26kd)-Sumo1 protein bacterial lysate.)

Product Categories/Family for anti-SUMO antibody

Antibodies; Ubiquitin

References

1. Yang, S.H., et al., Mol. Cell 13(4):611-617 (2004). 2. Bailey, D., et al., J. Biol. Chem. 279(1):692-703 (2004). 3. Ling, Y., et al., Nucleic Acids Res. 32(2):598-610 (2004). 4. Pountney, D.L., et al., Exp. Neurol. 184(1):436-446 (2003). 5. Ohshima, T., et al., J. Biol. Chem. 278(51):50833-50842 (2003). 6. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002). 7. Lapenta, V., et al., Genomics 40(2):362-366 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 1762973
• NCBI GeneID: 7341
• UniProt Accession #: P55854; P63165
• Molecular Weight: 11.5571108712116373
• NCBI Official Full Name: SUMO-1
• NCBI Official Synonym Full Names: small ubiquitin like modifier 1
• NCBI Official Symbol: SUMO1
• NCBI Official Synonym Symbols: DAP1; GMP1; PIC1; SMT3; UBL1; OFC10; SENP2; SMT3C; SMT3H3
• NCBI Protein Information: small ubiquitin-related modifier 1
• UniProt Protein Name: Small ubiquitin-related modifier 3
• Protein Family: SUMO-activating enzyme
• UniProt Gene Name: SUMO3
• UniProt Synonym Gene Names: SUMO-3Curated
• UniProt Entry Name: SUMO3_HUMAN

NCBI Description

This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

SUMO3: Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. Covalently attached to a number of proteins. Interacts with USP25 (via ts SIM domain); the interaction sumoylates USP25 and inhibits its ubiquitin hydrolyzing activity. Interacts with SAE2 and UBE2I. Expressed predominantly in liver. Belongs to the ubiquitin family. SUMO subfamily.

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: kinetochore; nucleoplasm; PML body; cytoplasm; nucleus

Molecular Function: protein binding; SUMO ligase activity

Biological Process: cellular protein metabolic process; protein sumoylation; post-translational protein modification