Western Blot (WB)
(Western blot analysis of extracts of human kidney tissue, using SUGT1 antibody.)
Rabbit SUGT1 Polyclonal Antibody | anti-SUGT1 antibody
SUGT1 Antibody
Gene Names
SUGT1; SGT1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
SUGT1; Polyclonal Antibody; SUGT1 Antibody; Protein 40 6 3; Protein 40-6-3; Protein SGT1 homolog; Putative 40 6 3 protein; Sgt1; SGT1 suppressor of G2 allele of SKP1; SGT1_HUMAN; SGT1B protein; sugt1; Suppressor of G2 allele of SKP1 homolog; Suppressor of G2 allele of SKP1 S. cerevisiae homolog of; anti-SUGT1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SUGT1 antibody detects endogenous levels of SUGT1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
365
Applicable Applications for anti-SUGT1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human SUGT1.
Subcellular Location
Cytoplasm. Nucleus. Translocates to the nucleus upon heat shock, requiring S100A6.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Sheep, Chicken
Similarity
Pig (100%), Zebrafish (83%), Bovine (100%), Sheep (100%), Chicken (92%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts of human kidney tissue, using SUGT1 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of human kidney tissue, using SUGT1 antibody.)
Immunohistochemistry (IHC)
(MBS9603709 at 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603709 at 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-SUGT1 antibody
Function: May play a role in ubiquitination and subsequent proteasomal degradation of target proteins.
Subunit Structure: Probably associates with SCF (SKP1-CUL1-F-box protein) complex through interaction with SKP1. Interacts with S100A6. Interacts with HSP90.
Post-translational Modifications: Phosphorylated at Ser-281 and Ser-331, dephosphorylation promotes nuclear translocation, most likely due to disruption of the SUGT1-HSP90 complex.
Similarity: The CS domain mediates interaction with HSP90. Belongs to the SGT1 family.
Subunit Structure: Probably associates with SCF (SKP1-CUL1-F-box protein) complex through interaction with SKP1. Interacts with S100A6. Interacts with HSP90.
Post-translational Modifications: Phosphorylated at Ser-281 and Ser-331, dephosphorylation promotes nuclear translocation, most likely due to disruption of the SUGT1-HSP90 complex.
Similarity: The CS domain mediates interaction with HSP90. Belongs to the SGT1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 38 kDa; 41 kDa
Predicted: 42 kDa
Predicted: 42 kDa
NCBI Official Full Name
protein SGT1 homolog isoform B
NCBI Official Synonym Full Names
SGT1 homolog, MIS12 kinetochore complex assembly cochaperone
NCBI Official Symbol
SUGT1
NCBI Official Synonym Symbols
SGT1
NCBI Protein Information
protein SGT1 homolog
UniProt Protein Name
Protein SGT1 homolog
UniProt Gene Name
SUGT1
NCBI Description
This gene encodes a highly conserved nuclear protein involved in kinetochore function and required for the G1/S and G2/M transitions. This protein interacts with heat shock protein 90. Alternative splicing results in multiple transcript variants. Pseudogenes for this gene have been defined on several different chromosomes. [provided by RefSeq, Mar 2016]
Uniprot Description
May play a role in ubiquitination and subsequent proteasomal degradation of target proteins.
Research Articles on SUGT1
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Product Notes
The SUGT1 sugt1 (Catalog #AAA9603709) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SUGT1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SUGT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the SUGT1 sugt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SUGT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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