Rabbit anti-Human SUFU Polyclonal Antibody | anti-SUFU antibody

SUFU (Suppressor of Fused Homolog, SUFUH, SUFUXL, UNQ650/PRO1280) (FITC)

Cat. Num. AAA6395590

• Gene Names: SUFU; SUFUH; SUFUXL; PRO1280
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SUFU; Polyclonal Antibody; SUFU (Suppressor of Fused Homolog; SUFUH; SUFUXL; UNQ650/PRO1280) (FITC); anti-SUFU antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SUFU.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-SUFU antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human SUFU, aa1-484, (NP_057253.2, 51684).
• Immunogen Sequence: MAELRPSGAPGPTAPPAPGPTAPPAFASLFPPGLHAIYGECRRLYPDQPNPLQVTAIVKYWLGGPDPLDYVSMYRNVGSPSANIPEHWHYISFGLSDLYGDNRVHEFTGTDGPSGFGFELTFRLKRETGESAPPTWPAELMQGLARYVFQSENTFCSGDHVSWHSPLDNSESRIQHMLLTEDPQMQPVQTPFGVVTFLQIVGVCTEELHSAQQWNGQGILELLRTVPIAGGPWLITDMRRGETIFEIDPHLQERVDKGIETDGSNLSGVSAKCAWDDLSRPPEDDEDSRSICIGTQPRRLSGKDTEQIRETLRRGLEINSKPVLPPINPQRQNGLAHDRAPSRKDSLESDSSTAIIPHELIRTRQLESVHLKFNQESGALIPLCLRGRLLHGRHFTYKSITGDMAITFVSTGVEGAFATEEHPYAAHGPWLQILLTEEFVEKMLEDLEDLTSPEEFKLPKEYSWPEKKLKVSILPDVVFDSPLH
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of SUFU expression in transfected 293T cell line by SUFU polyclonal antibody. Lane 1: SUFU transfected lysate (53.9kD). Lane 2: Non-transfected lysate.)

Testing Data

(Proximity Ligation Analysis (PLA) of protein-protein interactions between SUFU and GLI1 HeLa cells were stained with SUFU rabbit purified polyclonal 1:1200 and GLI1 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Product Categories/Family for anti-SUFU antibody

Antibodies; Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 19923479
• NCBI GeneID: 51684
• NCBI Accession #: NP_057253.2
• NCBI GenBank Nucleotide #: NM_016169.2
• UniProt Accession #: Q9UMX1; Q7LCP7; Q9NT90; Q9NZ07; Q9UHK2; Q9UHM8; Q9UMY0
• Molecular Weight: 52,977 Da
• NCBI Official Full Name: suppressor of fused homolog isoform 1
• NCBI Official Synonym Full Names: suppressor of fused homolog (Drosophila)
• NCBI Official Symbol: SUFU
• NCBI Official Synonym Symbols: SUFUH; SUFUXL; PRO1280
• NCBI Protein Information: suppressor of fused homolog
• UniProt Protein Name: Suppressor of fused homolog
• Protein Family: Suppressor of fused
• UniProt Gene Name: SUFU
• UniProt Synonym Gene Names: SUFUH
• UniProt Entry Name: SUFU_HUMAN

NCBI Description

The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

SUFU: a transcriptional regulator which negatively regulates the hedgehog signaling pathway. Down-regulates GLI1-mediated transactivation of target genes. Part of a corepressor complex that acts on DNA-bound GLI1. Modulates nuclear-cytoplasmic shuttling of Gli-1. May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome. Sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of FUSED to both SUFU and a GLI protein. Mutations in SUFU predispose to medulloblastoma. Part of a DNA-bound corepressor complex containing SAP18, GLI1 and SIN3. Part of a complex containing CTNNB1. Binds BTRC, GLI2, GLI3, SAP18 and STK36. Binds both free and DNA-bound GLI1. Defects in SUFU are a cause of medulloblastoma (MDB). MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children. Three alternatively spliced isoforms have been described.

Protein type: Transcription, coactivator/corepressor; Adaptor/scaffold; Tumor suppressor

Chromosomal Location of Human Ortholog: 10q24.32

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: signal transducer activity; protein binding; beta-catenin binding; transcription factor binding; transcription corepressor activity; protein kinase binding

Biological Process: skin development; cytoplasmic sequestering of transcription factor; multicellular organismal development; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription factor import into nucleus; proteolysis; signal transduction; regulation of transcription, DNA-dependent; negative regulation of smoothened signaling pathway; neural tube closure; negative regulation of osteoblast differentiation; heart looping; skeletal development; smoothened signaling pathway involved in ventral spinal cord interneuron specification; smoothened signaling pathway involved in spinal cord motor neuron cell fate specification

Disease: Meningioma, Familial, Susceptibility To; Basal Cell Nevus Syndrome; Medulloblastoma