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Western Blot (WB)
(Western blot analysis of 20 ug of whole cell lysate (A: mouse brain) using a 10% SDS PAGE gel and SUCLA2 antibody at a dilution of 1:20000)
Rabbit SUCLA2 Polyclonal Antibody | anti-SUCLA2 antibody
SUCLA2 Antibody
Gene Names
SUCLA2; A-SCS; A-BETA; MTDPS5; SCS-betaA
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Antigen-Affinity Chromatography
Synonyms
SUCLA2; Polyclonal Antibody; SUCLA2 Antibody; Affinity Purified Rabbit Polyclonal SUCLA2 Antibody; Polyclonal SUCLA2 antibody; Anti-SUCLA2 antibody; SUCLA2 antibody; succinyl-CoA synthetase beta-A chain antibody; SUCLA 2 antibody; SUCLA-2; SCS-betaA antibody; ATP-specific succinyl-CoA synthetase beta subunit antibody; mitochondrial succinyl-CoA ligase [ADP-forming] subunit beta antibody; MTDPS5 antibody; renal carcinoma antigen NY-REN-39 antibody; SUCLA-2 antibody; ATP-specific succinyl-CoA synthetase subunit beta antibody; SUCLA 2; (-)BETA antibody; anti-SUCLA2 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
463
Applicable Applications for anti-SUCLA2 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IF: 1:100-1:500
IHC-P: 1:100-1:500
ICC: 1:100-1:500
WB: 1:1000-1:20000
IHC-P: 1:100-1:500
ICC: 1:100-1:500
WB: 1:1000-1:20000
Cross-Reactivity
Human, Mouse
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of 20 ug of whole cell lysate (A: mouse brain) using a 10% SDS PAGE gel and SUCLA2 antibody at a dilution of 1:20000)
Western Blot (WB)
(Western blot analysis of 20 ug of whole cell lysate (A: mouse brain) using a 10% SDS PAGE gel and SUCLA2 antibody at a dilution of 1:20000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Hep G2; B: Molt-4) using a 10% SDS PAGE gel and SUCLA2 antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Hep G2; B: Molt-4) using a 10% SDS PAGE gel and SUCLA2 antibody at a dilution of 1:1000)
Immunofluorescence (IF)
(Immunofluorescence analysis of methanol-fixed A431, using SUCLA2 antibody at 1:200 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of methanol-fixed A431, using SUCLA2 antibody at 1:200 dilution.)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded Cal27 xenograft using SUCLA2 antibody at a dilution of 1:500)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded Cal27 xenograft using SUCLA2 antibody at a dilution of 1:500)
Related Product Information for anti-SUCLA2 antibody
Succinyl-CoA synthetase is a mitochondrial matrix enzyme that acts as a heterodimer, being composed of an invariant alpha subunit and a substrate-specific beta subunit. The protein encoded by this gene is an ATP-specific SCS beta subunit that dimerizes with the SCS alpha subunit to form SCS-A, an essential component of the tricarboxylic acid cycle. SCS-A hydrolyzes ATP to convert succinate to succinyl-CoA. Defects in this gene are a cause of myopathic mitochondrial DNA depletion syndrome. A pseudogene of this gene has been found on chromosome 6.
Product Categories/Family for anti-SUCLA2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50 kDa
NCBI Official Full Name
succinate--CoA ligase
NCBI Official Synonym Full Names
succinate-CoA ligase ADP-forming beta subunit
NCBI Official Symbol
SUCLA2
NCBI Official Synonym Symbols
A-SCS; A-BETA; MTDPS5; SCS-betaA
NCBI Protein Information
succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial
UniProt Protein Name
Succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial
UniProt Gene Name
SUCLA2
UniProt Synonym Gene Names
SCS-betaA
UniProt Entry Name
SUCB1_HUMAN
NCBI Description
Succinyl-CoA synthetase (SCS) is a mitochondrial matrix enzyme that acts as a heterodimer, being composed of an invariant alpha subunit and a substrate-specific beta subunit. The protein encoded by this gene is an ATP-specific SCS beta subunit that dimerizes with the SCS alpha subunit to form SCS-A, an essential component of the tricarboxylic acid cycle. SCS-A hydrolyzes ATP to convert succinate to succinyl-CoA. Defects in this gene are a cause of myopathic mitochondrial DNA depletion syndrome. A pseudogene of this gene has been found on chromosome 6. [provided by RefSeq, Jul 2008]
Research Articles on SUCLA2
Similar Products
Product Notes
The SUCLA2 sucla2 (Catalog #AAA5312274) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SUCLA2 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IF: 1:100-1:500 IHC-P: 1:100-1:500 ICC: 1:100-1:500 WB: 1:1000-1:20000. Researchers should empirically determine the suitability of the SUCLA2 sucla2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SUCLA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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