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Western Blot (WB) (STS Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the STS antibody detected the STS protein (arrow).)

Rabbit anti-Human STS Polyclonal Antibody | anti-STS antibody

STS, CT (STS, ARSC1, Steryl-sulfatase, Arylsulfatase C, Steroid sulfatase, Steryl-sulfate sulfohydrolase) (FITC)

Gene Names
STS; ES; ASC; XLI; ARSC; SSDD; ARSC1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
STS; Polyclonal Antibody; CT (STS; ARSC1; Steryl-sulfatase; Arylsulfatase C; Steroid sulfatase; Steryl-sulfate sulfohydrolase) (FITC); Steryl-sulfate sulfohydrolase; anti-STS antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-STS antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
STS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-517 amino acids from the C-terminal region of human STS.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(STS Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the STS antibody detected the STS protein (arrow).)

Western Blot (WB) (STS Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the STS antibody detected the STS protein (arrow).)

Immunohistochemistry (IHC)

(STS Antibody (C-term) (AP14527b)immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of STS Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (STS Antibody (C-term) (AP14527b)immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of STS Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-STS antibody
The protein encoded by this gene catalyzes the conversion of sulfated steroid precursors to estrogens during pregnancy. The encoded protein is found in the endoplasmic reticulum, where it acts as a homodimer. Mutations in this gene are known to cause X-linked ichthyosis (XLI).
Product Categories/Family for anti-STS antibody
References
Chanplakorn, N., et al. Breast Cancer Res. Treat. 120(3):639-648(2010). Gruber, R., et al. J. Dermatol. Sci. 58(1):72-75(2010). Canueto, J., et al. J Eur Acad Dermatol Venereol (2010). In press: Li, J., et al. Breast Cancer Res. 12 (2), R19 (2010): Chakrabarti, B., et al. Autism Res 2(3):157-177(2009).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
412
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
65,492 Da
NCBI Official Full Name
steryl-sulfatase
NCBI Official Synonym Full Names
steroid sulfatase (microsomal), isozyme S
NCBI Official Symbol
STS
NCBI Official Synonym Symbols
ES; ASC; XLI; ARSC; SSDD; ARSC1
NCBI Protein Information
steryl-sulfatase; arylsulfatase C; estrone sulfatase; steryl-sulfate sulfohydrolase
UniProt Protein Name
Steryl-sulfatase
Protein Family
UniProt Gene Name
STS
UniProt Synonym Gene Names
ARSC1; ASC
UniProt Entry Name
STS_HUMAN

NCBI Description

The protein encoded by this gene catalyzes the conversion of sulfated steroid precursors to estrogens during pregnancy. The encoded protein is found in the endoplasmic reticulum, where it acts as a homodimer. Mutations in this gene are known to cause X-linked ichthyosis (XLI). [provided by RefSeq, Jul 2008]

Uniprot Description

STS: Conversion of sulfated steroid precursors to estrogens during pregnancy. Defects in STS are the cause of ichthyosis X-linked (IXL). Ichthyosis X-linked is a keratinization disorder manifesting with mild erythroderma and generalized exfoliation of the skin within a few weeks after birth. Affected boys later develop large, polygonal, dark brown scales, especially on the neck, extremities, trunk, and buttocks. Belongs to the sulfatase family.

Protein type: Lipid Metabolism - androgen and estrogen; Hydrolase; Membrane protein, multi-pass; EC 3.1.6.2; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.32

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; membrane; intracellular membrane-bound organelle; lysosome; endoplasmic reticulum lumen; endoplasmic reticulum; plasma membrane; integral to membrane; endosome

Molecular Function: metal ion binding; sulfuric ester hydrolase activity; steryl-sulfatase activity

Biological Process: epidermis development; sphingolipid metabolic process; cellular protein metabolic process; glycosphingolipid metabolic process; female pregnancy; post-translational protein modification; steroid catabolic process

Disease: Ichthyosis, X-linked

Research Articles on STS

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Product Notes

The STS sts (Catalog #AAA6352239) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The STS, CT (STS, ARSC1, Steryl-sulfatase, Arylsulfatase C, Steroid sulfatase, Steryl-sulfate sulfohydrolase) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's STS can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the STS sts for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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