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Rabbit anti-Human STK39 Polyclonal Antibody | anti-STK39 antibody

Phospho-STK39 (Ser309) Antibody

Gene Names
STK39; DCHT; PASK; SPAK
Reactivity
Human
Applications
ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
STK39; Polyclonal Antibody; Phospho-STK39 (Ser309) Antibody; DCHT; DKFZp686K05124; OTTHUMP00000165175; PASK; Proline alanine rich STE20 related kinase; Serine threonine kinase 39 (STE20/SPS1 homolog yeast); Serine threonine kinase 39; Serine/threonine protein kinase 39; Serine/threonine-protein kinase 39; Small intestine SPAK like kinase; SPAK; Ste 20 related kinase; Ste-20-related kinase; Ste20 like protein kinase; STE20/SPS1 homolog; STE20/SPS1 related proline alanine rich protein kinase; STE20/SPS1-related proline-alanine-rich protein kinase; STK 39; Stk39; STK39_HUMAN; anti-STK39 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-STK39 (Ser309) Antibody detects endogenous levels of STK39 only when phosphorylated at Ser309.
Tissue Specificity: Predominantly expressed in brain and pancreas followed by heart, lung, kidney, skeletal muscle, liver, placenta and testis.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-STK39 antibody
Peptide ELISA (EIA)
Application Notes
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human STK39 around the phosphorylation site of Ser309.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Phosphorylated at Ser-309 by PRKCQ. Autophosphorylation at Thr-231 positively regulates its activity (By similarity).
Subunit Structure
The phosphorylated form forms a complex with WNK2 (By similarity). Interacts with RELT (By similarity). Interacts with SORL1 (via cytosolic C-terminus).
Similarity
PAPA box (proline-alanine repeats) may target the kinase to a specific subcellular location by facilitating interaction with intracellular proteins such as actin or actin-like proteins.Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Subcellular Location
Cytoplasm. Nucleus.
Note: Nucleus when caspase-cleaved.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Related Product Information for anti-STK39 antibody
May act as a mediator of stress-activated signals. Mediates the inhibition of SLC4A4, SLC26A6 as well as CFTR activities by the WNK scaffolds, probably through phosphorylation. Phosphorylates RELT.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
59,474 Da
NCBI Official Full Name
STE20/SPS1-related proline-alanine-rich protein kinase
NCBI Official Synonym Full Names
serine threonine kinase 39
NCBI Official Symbol
STK39
NCBI Official Synonym Symbols
DCHT; PASK; SPAK
NCBI Protein Information
STE20/SPS1-related proline-alanine-rich protein kinase; STE20/SPS1 homolog; ste-20-related kinase; Ste20-like protein kinase; small intestine SPAK-like kinase; serine/threonine-protein kinase 39; proline-alanine-rich STE20-related kinase; serine threonine
UniProt Protein Name
STE20/SPS1-related proline-alanine-rich protein kinase
UniProt Gene Name
STK39
UniProt Synonym Gene Names
SPAK; Ste-20-related kinase
UniProt Entry Name
STK39_HUMAN

NCBI Description

This gene encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress. [provided by RefSeq, Jul 2008]

Uniprot Description

STLK3: May act as a mediator of stress-activated signals. The phosphorylated form forms a complex with WNK2. Predominantly expressed in brain and pancreas followed by heart, lung, kidney, skeletal muscle, liver, placenta and testis. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Protein type: Kinase, protein; Protein kinase, STE; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; FRAY subfamily

Chromosomal Location of Human Ortholog: 2q24.3

Cellular Component: cytoskeleton; basolateral plasma membrane; cytoplasm; apical plasma membrane; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein kinase binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: peptidyl-serine phosphorylation; regulation of inflammatory response; positive regulation of potassium ion transport; peptidyl-threonine phosphorylation; response to stress; protein amino acid phosphorylation

Research Articles on STK39

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Product Notes

The STK39 stk39 (Catalog #AAA9613009) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-STK39 (Ser309) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's STK39 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the STK39 stk39 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STK39, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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