Chemiluminescence
(Y1 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SF-1 (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates SF-1 (~53kD).)
Rabbit anti-Mouse Steroidogenic Factor 1 Polyclonal Antibody | anti-NR5A1 antibody
Steroidogenic Factor 1 (SF-1, NR5A1)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: Immunoblot Analysis: 0.5-2ug/ml of this lot detected SF-1 in RIPA lysates from Y1 cells.
Chemiluminescence
(Y1 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SF-1 (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates SF-1 (~53kD).)
Chemiluminescence
(Y1 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SF-1 (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates SF-1 (~53kD).)
SF-1 has been shown to promote transcriptional activity of many steroidogenic enzymes from the cytochrome P450 family. SF-1 contains two activation function domains, designated AF1 and AF2, common to many nuclear steroid receptors which have been shown to be necessary for interaction with other nuclear receptors as well as coactivators and corepressors including steroid receptor coactivator-1 (SRC-1), glucocorticoid receptor interacting protein 1 (GRIP1), cAMP response element binding protein (CREB) binding protein (CBP) and silencing mediator of retinoid acid and thyroid hormone receptor (SMRT).
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a transcriptional activator involved in sex determination. The encoded protein binds DNA as a monomer. Defects in this gene are a cause of XY sex reversal with or without adrenal failure as well as adrenocortical insufficiency without ovarian defect. [provided by RefSeq]
Uniprot Description
STF-1: a nuclear hormone receptor of the NR5 subfamily. Essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P-450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the Muellerian inhibiting substance (AMH) gene as well as the AHCH and STAR genes.
Protein type: Nuclear receptor
Chromosomal Location of Human Ortholog: 9q33
Cellular Component: nucleoplasm; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; ligand-dependent nuclear receptor activity; enzyme binding; DNA binding; zinc ion binding; sequence-specific DNA binding; transcription coactivator activity; double-stranded DNA binding; steroid hormone receptor activity; phospholipid binding; chromatin binding
Biological Process: tissue development; transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; adrenal gland development; primary sex determination; male gonad development; multicellular organismal aging; regulation of steroid biosynthetic process; cell-cell signaling; luteinization; hormone metabolic process; steroid hormone mediated signaling; gene expression; positive regulation of transcription from RNA polymerase II promoter; maintenance of protein localization in nucleus; cell differentiation
Disease: Premature Ovarian Failure 7; 46,xy Sex Reversal 3; Spermatogenic Failure 8
Research Articles on NR5A1
Similar Products
Product Notes
The NR5A1 nr5a1 (Catalog #AAA616024) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Steroidogenic Factor 1 (SF-1, NR5A1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Steroidogenic Factor 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Immunoblot Analysis: 0.5-2ug/ml of this lot detected SF-1 in RIPA lysates from Y1 cells. Researchers should empirically determine the suitability of the NR5A1 nr5a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Steroidogenic Factor 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
