Rabbit anti-Human, Mouse STEAP3 Polyclonal Antibody | anti-STEAP3 antibody

STEAP3 Polyclonal Antibody

Cat. Num. AAA3008580

• Gene Names: STEAP3; STMP3; TSAP6; pHyde; AHMIO2; dudlin-2; dudulin-2
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: STEAP3; Polyclonal Antibody; STEAP3 Polyclonal Antibody; STMP3; TSAP6; Dudulin-2; Dudulin 2; Dudulin2; anti-STEAP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: STEAP3 polyclonal antibody detects endogenous levels of STEAP3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 488

• Applicable Applications for anti-STEAP3 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant protein of human STEAP3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using STEAP3 antibody.)

Related Product Information for anti-STEAP3 antibody

Caspases modulate apoptosis in various ways. Specifically, Caspase-3, a death protease, is instrumental in cleaving cellular proteins, dismantling the cell and forming apoptotic bodies. pHyde has a potential role as a tumor suppressor by inducing caspase-3-mediated apoptosis and stimulating p53 expression. A dose-dependent increase in caspase-3 activity is observed in transduced pHyde DU145 cells. Furthermore, caspase-3 may be necessary for pHyde-mediated apoptosis. The pHyde gene may upregulate the apoptosis pathway and thus have a potential application in cancer gene therapy. Recombinant pHyde inhibits the growth of human prostate cancer cell lines DU145 and LNCaP in vitro. DU145 tumors may be reduced significantly in vivo when nude mice are injected with recombinant pHyde. pHyde also has a demonstrated growth inhibitory effect on human breast cancer cells. This suggests that pHyde may have a role in inhibiting different tumor types.

NCBI and Uniprot Product Information

• NCBI GI #: 56549147
• NCBI GeneID: 55240
• NCBI Accession #: NP_001008410.1
• NCBI GenBank Nucleotide #: NP_001008410.1
• UniProt Accession #: Q658P3
• Molecular Weight: ~ 55kDa
• NCBI Official Full Name: metalloreductase STEAP3 isoform b
• NCBI Official Synonym Full Names: STEAP3 metalloreductase
• NCBI Official Symbol: STEAP3
• NCBI Official Synonym Symbols: STMP3; TSAP6; pHyde; AHMIO2; dudlin-2; dudulin-2
• NCBI Protein Information: metalloreductase STEAP3
• UniProt Protein Name: Metalloreductase STEAP3
• Protein Family: Metalloreductase
• UniProt Gene Name: STEAP3
• UniProt Synonym Gene Names: TSAP6; hTSAP6; hpHyde
• UniProt Entry Name: STEA3_HUMAN

NCBI Description

This gene encodes a multipass membrane protein that functions as an iron transporter. The encoded protein can reduce both iron (Fe3+) and copper (Cu2+) cations. This protein may mediate downstream responses to p53, including promoting apoptosis. Deficiency in this gene can cause anemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

Uniprot Description

STEAP3: a multi-pass membrane protein induced directly by p53. Plays an important role in facilitating exosome secretion. Enhances secretion in the DNA damage-induced p53-dependent exosomal secretory pathway. Localizes to vesicular-like structures at the plasma membrane and around the nucleus. Highly expressed in hematopoietic tissues where it localizes to the specialized endosome. An endosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Participates in erythroid iron homeostasis by reducing Fe(3+) to Fe(2+). Can also reduce of Cu(2+) to Cu(1+), suggesting that it participates in copper homeostasis. Uses NAD(+) as acceptor. Contains a highly-conserved tyrosine-based motif that has been shown to target transmembrane proteins, including LAMP-1, LAMP-2, the TfR, and SLC11A1 to endosomes, lysosomes, and lysosome-related organelles. Facilitates the exosomal secretion of proteins such as TCTP. Interacts with BNIP3L, MYT1 and TPT1. Four human isoforms are produced by alternative splicing. Isoform 4 is also known as pHyde II.

Protein type: Membrane protein, integral; Oxidoreductase; Membrane protein, multi-pass; Transporter, ion channel; Transporter, iron; EC 1.16.1.-; Transporter

Chromosomal Location of Human Ortholog: 2q14.2

Cellular Component: multivesicular body; integral to membrane; plasma membrane; endosome membrane

Molecular Function: protein binding; metal ion binding; cupric reductase activity; ferric-chelate reductase activity

Biological Process: apoptosis; cellular iron ion homeostasis; protein secretion; copper ion import; transferrin transport; cell cycle; transmembrane transport

Disease: Anemia, Hypochromic Microcytic, With Iron Overload 2