Rabbit anti-Human STAT1 Polyclonal Antibody | anti-STAT1 antibody

STAT1, Phosphorylated (Ser727) (Signal Transducer And Activator Of Transcription 1-alpha/beta, Transcription Factor ISGF-3 Components p91/p84) (Azide free) (HRP)

Cat. Num. AAA6500895

• Gene Names: STAT1; CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: STAT1; Polyclonal Antibody; Phosphorylated (Ser727) (Signal Transducer And Activator Of Transcription 1-alpha/beta; Transcription Factor ISGF-3 Components p91/p84) (Azide free) (HRP); anti-STAT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human STAT1 when phosphorylated at Ser727.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-STAT1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser727 of human STAT1 (KLH).
• Conjugate: HRP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-Phospho-STAT1 Pab in mouse brain tissue lysate (35ug/lane). Phospho-STAT1(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-STAT1 antibody

Antibodies; Transcription Factors; STAT

References

1. Garcin, D., et al., J. Virol. 78(16):8799-8811 (2004). 2. Melen, K., et al., J. Med. Virol. 73(4):536-547 (2004). 3. Klampfer, L., et al., J. Biol. Chem. 279(29):30358-30368 (2004). 4. Marg, A., et al., J. Cell Biol. 165(6):823-833 (2004). 5. Chim, C.S., et al., Blood 103(12):4630-4635 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 6274552
• NCBI GeneID: 6772
• NCBI Accession #: NP_009330
• NCBI GenBank Nucleotide #: NM_007315.3
• UniProt Accession #: P42224; Q53S88; Q53XW4; Q68D00; Q9UDL5; A8K989; B2RCA0; D2KFR8; D3DPI7
• Molecular Weight: 91 kDa
• NCBI Official Full Name: signal transducer and activator of transcription 1-alpha/beta isoform alpha
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 1, 91kDa
• NCBI Official Symbol: STAT1
• NCBI Official Synonym Symbols: CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
• NCBI Protein Information: signal transducer and activator of transcription 1-alpha/beta; transcription factor ISGF-3 components p91/p84; signal transducer and activator of transcription-1; signal transducer and activator of transcription 1, 91kD
• UniProt Protein Name: Signal transducer and activator of transcription 1-alpha/beta
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: STAT1
• UniProt Entry Name: STAT1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

STAT1: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of certain receptor tyrosine kinases, GPCRs, and receptors for various interleukins and interferons. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 2q32.2

Cellular Component: nucleoplasm; axon; nuclear chromatin; cytoplasm; dendrite; nucleolus; cytosol; nucleus

Molecular Function: identical protein binding; signal transducer activity; protein binding; protein homodimerization activity; enzyme binding; double-stranded DNA binding; transcription factor activity; tumor necrosis factor receptor binding; CCR5 chemokine receptor binding

Biological Process: transcription from RNA polymerase II promoter; response to peptide hormone stimulus; response to cAMP; viral reproduction; blood circulation; apoptosis; positive regulation of transcription, DNA-dependent; positive regulation of smooth muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; regulation of apoptosis; response to exogenous dsRNA; tumor necrosis factor-mediated signaling pathway; positive regulation of mesenchymal cell proliferation; negative regulation of viral protein levels in host cell; lipopolysaccharide-mediated signaling pathway; defense response to virus; response to nutrient; response to drug; cytokine and chemokine mediated signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; JAK-STAT cascade; regulation of transcription from RNA polymerase II promoter; negative regulation of angiogenesis; response to hydrogen peroxide; cellular response to insulin stimulus; response to mechanical stimulus; response to cytokine stimulus; negative regulation of endothelial cell proliferation; endothelial cell migration; positive regulation of transcription from RNA polymerase II promoter

Disease: Immunodeficiency 31a; Immunodeficiency 31b; Immunodeficiency 31c