Western Blot (WB)
(Western blot analysis of ST8SIA4 Antibody (Center) in HL-60 cell line lysates (35ug/lane). ST8SIA4 (arrow) was detected using the purified Pab. Western blot analysis of ST8SIA4 Antibody (Center) in mouse spleen tissue lysates (35ug/lane). ST8SIA4 (arrow) was detected using the purified Pab.)
Rabbit anti-Human, Mouse ST8SIA4 Polyclonal Antibody | anti-st8sia4 antibody
ST8SIA4, ID (ST8SIA4, PST, PST1, SIAT8D, CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase, Alpha-2,8-sialyltransferase 8D, Polysialyltransferase-1, Sialyltransferase 8D, Sialytransferase St8Sia IV)
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
ST8SIA4; Polyclonal Antibody; ID (ST8SIA4; PST; PST1; SIAT8D; CMP-N-acetylneuraminate-poly-alpha-2; 8-sialyltransferase; Alpha-2; 8-sialyltransferase 8D; Polysialyltransferase-1; Sialyltransferase 8D; Sialytransferase St8Sia IV); Anti -ST8SIA4; anti-st8sia4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Human, mouse
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-st8sia4 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Immunogen
ST8SIA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 193~222 amino acids from the Central region of human ST8SIA4.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of ST8SIA4 Antibody (Center) in HL-60 cell line lysates (35ug/lane). ST8SIA4 (arrow) was detected using the purified Pab. Western blot analysis of ST8SIA4 Antibody (Center) in mouse spleen tissue lysates (35ug/lane). ST8SIA4 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of ST8SIA4 Antibody (Center) in HL-60 cell line lysates (35ug/lane). ST8SIA4 (arrow) was detected using the purified Pab. Western blot analysis of ST8SIA4 Antibody (Center) in mouse spleen tissue lysates (35ug/lane). ST8SIA4 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(ST8SIA4 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ST8SIA4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(ST8SIA4 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ST8SIA4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(ST8SIA4 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(ST8SIA4 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-st8sia4 antibody
The protein encoded by this gene catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid, a modulator of the adhesive properties of neural cell adhesion molecule (NCAM1). The encoded protein, which is a member of glycosyltransferase family 29, is a type II membrane protein that may be present in the Golgi apparatus.
Product Categories/Family for anti-st8sia4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4
NCBI Official Synonym Full Names
ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4
NCBI Official Symbol
st8sia4
NCBI Protein Information
ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4; siat 8F; alpha 2,8-sialyltransferase ST8Sia VI
Protein Family
Similar Products
Product Notes
The st8sia4 (Catalog #AAA642688) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ST8SIA4, ID (ST8SIA4, PST, PST1, SIAT8D, CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase, Alpha-2,8-sialyltransferase 8D, Polysialyltransferase-1, Sialyltransferase 8D, Sialytransferase St8Sia IV) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ST8SIA4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the st8sia4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ST8SIA4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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