Rabbit anti-Human ST5 Polyclonal Antibody | anti-ST5 antibody

ST5, CT (ST5, DENND2B, HTS1, Suppression of tumorigenicity 5 protein, DENN domain-containing protein 2B, HeLa tumor suppression 1) (PE)

Cat. Num. AAA6351542

• Gene Names: ST5; HTS1; p126; DENND2B
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ST5; Polyclonal Antibody; CT (ST5; DENND2B; HTS1; Suppression of tumorigenicity 5 protein; DENN domain-containing protein 2B; HeLa tumor suppression 1) (PE); HeLa tumor suppression 1; anti-ST5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ST5 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ST5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1108~1137 amino acids from the C-terminal region of human ST5.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ST5 Antibody (C-term K1121) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the ST5 antibody detected the ST5 protein (arrow).)

Flow Cytometry (FC/FACS)

(ST5 Antibody (C-term) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ST5 antibody

ST5 was identified by its ability to suppress the tumorigenicity of Hela cells in nude mice. The protein contains a C-terminal region that shares similarity with the Rab 3 family of small GTP binding proteins. This protein preferentially binds to the SH3 domain of c-Abl kinase, and acts as a regulator of MAPK1/ERK2 kinase, which may contribute to its ability to reduce the tumorigenic phenotype in cells.

Product Categories/Family for anti-ST5 antibody

Antibodies; Cancer Markers

References

Majidi, M., et al., J. Biol. Chem. 275(9):6560-6565 (2000). Hubbs, A.E., et al., Oncogene 18(15):2519-2525 (1999). Majidi, M., et al., J. Biol. Chem. 273(26):16608-16614 (1998). Lichy, J.H., et al., Nucleic Acids Res. 24(23):4700-4708 (1996). Lichy, J.H., et al., Cell Growth Differ. 3(8):541-548 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 47132529
• NCBI GeneID: 6764
• NCBI Accession #: NP_005409
• NCBI GenBank Nucleotide #: NM_005418
• UniProt Accession #: P78524
• Molecular Weight: 126kDa
• NCBI Official Full Name: suppression of tumorigenicity 5 protein isoform 1
• NCBI Official Synonym Full Names: suppression of tumorigenicity 5
• NCBI Official Symbol: ST5
• NCBI Official Synonym Symbols: HTS1; p126; DENND2B
• NCBI Protein Information: suppression of tumorigenicity 5 protein
• UniProt Protein Name: Suppression of tumorigenicity 5 protein
• Protein Family: Suppression of tumorigenicity 5 protein
• UniProt Gene Name: ST5
• UniProt Synonym Gene Names: DENND2B; HTS1
• UniProt Entry Name: ST5_HUMAN

NCBI Description

This gene was identified by its ability to suppress the tumorigenicity of Hela cells in nude mice. The protein encoded by this gene contains a C-terminal region that shares similarity with the Rab 3 family of small GTP binding proteins. This protein preferentially binds to the SH3 domain of c-Abl kinase, and acts as a regulator of MAPK1/ERK2 kinase, which may contribute to its ability to reduce the tumorigenic phenotype in cells. Three alternatively spliced transcript variants of this gene encoding distinct isoforms are identified. [provided by RefSeq, Jul 2008]

Uniprot Description

DENND2B: a guanine nucleotide exchange factor (GEF) that may activate RAB9A and RAB9B. May be involved in cytoskeletal organization and tumorogenicity. Widely expressed with the exception of peripheral blood lymphocytes and lymphoid cell lines. 3 isoforms of the human protein are produced by alternative promoter. Isoform 1 interacts with the SH3 domain of ABL1, is expressed in several epithelial, fibroblast, and tumorigenic cell lines, and seems to be involved in the activation of ERK2. Isoform 3, expressed in primary cells that may be weakly tumorigenic, but not in tumorigenic cell lines, may block ERK2 activation stimulated by ABL1, and may alter cell morphology and cell growth.

Protein type: GEFs; GEFs, Rab

Chromosomal Location of Human Ortholog: 11p15

Molecular Function: Rab guanyl-nucleotide exchange factor activity