Rabbit SSH2 Polyclonal Antibody | anti-SSH2 antibody

SSH2 Polyclonal Antibody

Cat. Num. AAA3009374

• Gene Names: SSH2; SSH-2; SSH-2L
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SSH2; Polyclonal Antibody; SSH2 Polyclonal Antibody; Protein phosphatase Slingshot homolog 2; SSH-like protein 2; SSH-2L; hSSH-2L; KIAA1725; SSH2L; anti-SSH2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SSH2 polyclonal antibody detects endogenous levels of SSH2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 195

• Applicable Applications for anti-SSH2 antibody: Western Blot (WB)
• Application Notes: WB: 1:200-1:500
• Immunogen: Recombinant protein of human SSH2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cells, using SSH2 antibody.)

Related Product Information for anti-SSH2 antibody

SSH2 (slingshot homolog 2) is a 1, 423 amino acid protein that localizes to both the cytoplasm and the cytoskeleton and is a human homolog of the Drosophila slingshot (ssh) protein. Containing one tyrosine-protein phosphatase domain, SSH2 functions as a protein phosphatase that regulates Actin filament dynamics via the dephosphorylation of target proteins, such as Cofilin, which mediate Actin filament assembly and disassembly. Four isoforms of SSH2 exist due to alternative splicing events. The gene encoding SSH2 maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1, 200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Malfunction or loss of p53 expression is associated with malignant cell growth and Li-Fraumeni syndrome.

NCBI and Uniprot Product Information

• NCBI GI #: 45829588
• NCBI GeneID: 85464
• UniProt Accession #: Q76I76
• Molecular Weight: ~ 158kDa
• NCBI Official Full Name: SSH2 protein
• NCBI Official Synonym Full Names: slingshot protein phosphatase 2
• NCBI Official Symbol: SSH2
• NCBI Official Synonym Symbols: SSH-2; SSH-2L
• NCBI Protein Information: protein phosphatase Slingshot homolog 2
• UniProt Protein Name: Protein phosphatase Slingshot homolog 2
• Protein Family: Protein phosphatase Slingshot
• UniProt Gene Name: SSH2
• UniProt Synonym Gene Names: KIAA1725; SSH2L; SSH-2L; hSSH-2L
• UniProt Entry Name: SSH2_HUMAN

NCBI Description

This gene encodes a protein tyrosine phosphatase that plays a key role in the regulation of actin filaments. The encoded protein dephosphorylates and activates cofilin, which promotes actin filament depolymerization. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

SSH2: Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein. Belongs to the protein-tyrosine phosphatase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Actin-binding; EC 3.1.3.16; EC 3.1.3.48; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: extracellular space; cytoskeleton; cytoplasm

Molecular Function: DNA binding; protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity; actin binding; phosphoprotein phosphatase activity

Biological Process: regulation of actin polymerization and/or depolymerization; protein amino acid dephosphorylation; regulation of axonogenesis; actin cytoskeleton organization and biogenesis