Immunofluorescence (IF)/Immunocytochemistry (ICC)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Rabbit SSFA2 Polyclonal Antibody | anti-SSFA2 antibody
SSFA2 Antibody
Gene Names
ITPRID2; CS1; CS-1; KRAP; SSFA2; SPAG13
Reactivity
Human, Mouse, RatPredicted: Pig
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
SSFA2; Polyclonal Antibody; SSFA2 Antibody; Cleavage signal-1 protein; CS-1; CS1; Ki-ras-induced actin-interacting protein; KIAA1927; KRAP; KRAS induced actin interacting protein; SPAG13; Sperm associated antigen 13; Sperm-specific antigen 2; SSFA2_HUMAN; anti-SSFA2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig
Predicted: Pig
Clonality
Polyclonal
Isotype
IgG
Specificity
SSFA2 Antibody detects endogenous levels of SSFA2.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1259
Applicable Applications for anti-SSFA2 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human SSFA2.
Tissue Specificity
Strongly expressed in pancreas and testis. Present in colon cancer cells (at protein level).
Fragment
Fab Fragment
Conjugate
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Western Blot (WB)
(Western blot analysis of extracts from Mouse testis tissue, using SSFA2 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Mouse testis tissue, using SSFA2 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-SSFA2 antibody
A 33 kDa peptide corresponding to the C-terminus of this protein is found in the testis and seems to be cleaved into 2 peptides of 14 kDa and 18 kDa found on the surface of mature sperm cells. This sperm surface antigen may be involved in some step of early cleavage of the fertilized oocyte.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed: 180 kDa
Predicted: 139 kDa
Predicted: 139 kDa
NCBI Official Full Name
SSFA2 protein
NCBI Official Synonym Full Names
ITPR interacting domain containing 2
NCBI Official Symbol
ITPRID2
NCBI Official Synonym Symbols
CS1; CS-1; KRAP; SSFA2; SPAG13
NCBI Protein Information
protein ITPRID2
UniProt Protein Name
Sperm-specific antigen 2
Protein Family
UniProt Gene Name
SSFA2
UniProt Synonym Gene Names
CS1; KIAA1927; KRAP; CS-1
UniProt Entry Name
SSFA2_HUMAN
Uniprot Description
SSFA2: 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 2q31.3
Cellular Component: nucleoplasm; cytoplasm; plasma membrane; nucleus
Molecular Function: actin binding; receptor binding
Research Articles on SSFA2
Similar Products
Product Notes
The SSFA2 ssfa2 (Catalog #AAA9611402) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SSFA2 Antibody reacts with Human, Mouse, Rat Predicted: Pig and may cross-react with other species as described in the data sheet. AAA Biotech's SSFA2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the SSFA2 ssfa2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SSFA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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