Rabbit anti-Human SSB Polyclonal Antibody | anti-SSB antibody

SSB (Phospho-S366) Polyclonal Antibody

Cat. Num. AAA3000111

• Gene Names: SSB; La; LARP3; La/SSB
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >110% (by SDS-PAGE).
• Synonyms: SSB; Polyclonal Antibody; SSB (Phospho-S366) Polyclonal Antibody; Autoantigen La; La; La autoantigen; La autoantigen homolog; La protein; La ribonucleoprotein; La ribonucleoprotein domain family member 3; LA; LARP3; Lupus La antigen; Lupus La protein; Lupus La protein homolog; mRNA for autoantigen; Sjoegren syndrome type B antigen; Sjogren syndrome antigen B; SS B; SS-B; SS-B/La protein; anti-SSB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-SSB (S366) polyclonal antibody detects endogenous levels of SSB protein only when phosphorylated at Serine 366.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >110% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 408

• Applicable Applications for anti-SSB antibody: Western Blot (WB)
• Application Notes: WB: 1:1000-1:2000
• Immunogen: Synthetic phosphopeptide derived from human SSB around the phosphorylation site of Serine 366.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of p-SSB (S366) polyclonal antibody at 1:1000 dilutionLane1:HEK293T whole cell lysateLane2:HEK293T treated with EGF (0.1ng/ml,15min) whole cell lysateLane3:HEK293T treated with EGF (0.1ng/ml,30min) whole cell lysateLane4:HEK293T treated with EGF (0.1ng/ml,60min) whole cell lysate)

Western Blot (WB)

(Western Blot (WB) analysis of P-SSB (S366) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:K562 whole cell lysate (40ug) Lane4:U-87MG whole cell lysate (40ug) Lane5:MCF-7 whole cell lysate (40ug))

Related Product Information for anti-SSB antibody

Ro/SSA is a ribonucleoprotein that binds to autoantibodies in 35 to 50% of patients with SLE and in up to 97% of patients with Sjogren syndrome. The Ro/SSA particle consists of a single immunoreactive protein noncovalently bound with one of four small RNA molecules. Most anti-Ro/SSA-positive sera have antibodies not only against the immunoreactive protein, but also against an Ro/SSA protein. The genes which encode the two proteins map to human chromosomes 11p15.5 and 1q31, respectively. La/SSB is an autoimmune RNA-binding protein that plays a role in the transcription of RNA polymerase III was originally defined by its reactivity with autoantibodies from patients with Sjogren syndrome and SLE.

NCBI and Uniprot Product Information

• NCBI GI #: 10835067
• NCBI GeneID: 6741
• NCBI Accession #: NP_003133.1
• NCBI GenBank Nucleotide #: NP_003133.1
• UniProt Accession #: P05455
• Molecular Weight: ~ 52kDa
• NCBI Official Full Name: lupus La protein
• NCBI Official Synonym Full Names: small RNA binding exonuclease protection factor La
• NCBI Official Symbol: SSB
• NCBI Official Synonym Symbols: La; LARP3; La/SSB
• NCBI Protein Information: lupus La protein
• UniProt Protein Name: Lupus La protein
• Protein Family: SSB protein
• UniProt Gene Name: SSB
• UniProt Synonym Gene Names: SS-B
• UniProt Entry Name: LA_HUMAN

NCBI Description

The protein encoded by this gene is involved in diverse aspects of RNA metabolism, including binding and protecting poly(U) termini of nascent RNA polymerase III transcripts from exonuclease digestion, processing 5' and 3' ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. Autoantibodies reacting with this protein are found in the sera of patients with Sjogren syndrome and systemic lupus erythematosus. Alternative promoter usage results in two different transcript variants which encode the same protein. [provided by RefSeq, Jun 2014]

Uniprot Description

SSB: a nuclear protein that plays a role in the transcription of RNA polymerase III. It is most probably a transcription termination factor. Binds to the 3' termini of virtually all nascent polymerase III transcripts. It is associated with precursor forms of RNA polymerase III transcripts including tRNA and 4.5S, 5s, 7s, and 7-2 RNAs.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: ribonucleoprotein complex; nucleus

Molecular Function: mRNA binding; protein binding; nucleotide binding; tRNA binding

Biological Process: RNA processing; histone mRNA metabolic process; tRNA modification