Rabbit anti-Human, Mouse SRPK2 Polyclonal Antibody | anti-SRPK2 antibody

Phospho-SRPK2 (Thr492) Antibody

Cat. Num. AAA9612700

• Gene Names: SRPK2; SFRSK2
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: SRPK2; Polyclonal Antibody; Phospho-SRPK2 (Thr492) Antibody; Human serine kinase SRPK2 mRNA; complete cds; Serine kinase SRPK2; Serine/arginine rich protein specific kinase 2; Serine/arginine-rich protein-specific kinase 2; Serine/threonine protein kinase SRPK2; SFRS protein kinase 2; SFRSK2; SR protein specific kinase 2; SR-protein-specific kinase 2; SRPK2_HUMAN; SRSF protein kinase 2 C-terminal; anti-SRPK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-SRPK2 (Thr492) Antibody detects endogenous levels of SRPK2 only when phosphorylated at Thr492.; Tissue Specificity: Highly expressed in brain, moderately expressed in heart and skeletal muscle and at low levels in lung, liver, and kidney.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-SRPK2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
• Application Notes: IHC: 1:50-1:200; IF/ICC: 1:100-1:500; WB: 1:500-1:2000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human SRPK2 around the phosphorylation site of Thr492.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylation at Thr-492 by PKB/AKT1 enhances its stimulatory activity in triggering cyclin-D1 (CCND1) expression and promoting apoptosis in neurons, which can be blocked by YWHAB. It also enhances its protein kinase activity toward ACIN1 and SRSF2, promotes its nuclear translocation and prevents its proteolytic cleavage.Proteolytically cleaved at Asp-139 and Asp-403 by caspase-3 during apoptotic cell death. Cleavage at Asp-139 which is the major site of cleavage, produces a small N-terminal fragment that translocates into nucleus and promotes VP16-induced apoptosis.
• Subunit Structure: Interacts with PKB/AKT1 in a phosphorylation-dependent manner. The phosphorylated form (by PKB/AKT1) interacts with YWHAB and YWHAE. Interaction with YWHAB suppresses its cleavage by caspases and inhibits the release of its N-terminal pro-apoptotic fragment. Interacts with SFN. Associates with U4/U6-U5 tri-small nuclear ribonucleoproteins (U4/U6-U5 tri-snRNPs).
• Similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.
• Subcellular Location: Cytoplasm. Nucleus.; Note: Shuttles between the nucleus and the cytoplasm. KAT5/TIP60 inhibits its nuclear translocation. Phosphorylation at Thr-492 by PKB/AKT1 promotes nuclear translocation.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Mouse brain, using Phospho-SRPK2 (Thr492) Antibody. The lane on the left was treated with blocking peptide.)

Immunohistochemistry (IHC)

(Staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)

(At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)

(At 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Testing Data

(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

Related Product Information for anti-SRPK2 antibody

Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Promotes neuronal apoptosis by up-regulating cyclin-D1 (CCND1) expression. This is done by the phosphorylation of SRSF2, leading to the suppression of p53/TP53 phosphorylation thereby relieving the repressive effect of p53/TP53 on cyclin-D1 (CCND1) expression. Phosphorylates ACIN1, and redistributes it from the nuclear speckles to the nucleoplasm, resulting in cyclin A1 but not cyclin A2 up-regulation. Plays an essential role in spliceosomal B complex formation via the phosphorylation of DDX23/PRP28. Can mediate hepatitis B virus (HBV) core protein phosphorylation. Plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles.

NCBI and Uniprot Product Information

• NCBI GI #: 33188447
• NCBI GeneID: 6733
• NCBI Accession #: NP_872633
• NCBI GenBank Nucleotide #: NM_182691
• UniProt Accession #: P78362; O75220; O75221; Q6NUL0; Q6V1X2; Q8IYQ3; A8MVX2
• Molecular Weight: 79,029 Da
• NCBI Official Full Name: SRSF protein kinase 2 isoform b
• NCBI Official Synonym Full Names: SRSF protein kinase 2
• NCBI Official Symbol: SRPK2
• NCBI Official Synonym Symbols: SFRSK2
• NCBI Protein Information: SRSF protein kinase 2; SFRS protein kinase 2; SR protein kinase 2; SR-protein-specific kinase 2; SRPK2; serine kinase SRPK2; serine/arginine-rich protein-specific kinase 2; serine/arginine-rich splicing factor kinase 2; serine/threonine-protein kinase SRP
• UniProt Protein Name: SRSF protein kinase 2
• Protein Family: SRSF protein kinase
• UniProt Gene Name: SRPK2
• UniProt Synonym Gene Names: SR-protein-specific kinase 2
• UniProt Entry Name: SRPK2_HUMAN