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Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using SRF Antibody. The picture on the right is blocked with the synthesized peptide.)

Rabbit SRF Polyclonal Antibody | anti-SRF antibody

SRF Antibody

Gene Names
SRF; MCM1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Synonyms
SRF; Polyclonal Antibody; SRF Antibody; anti-SRF antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
SRF Antibody detects endogenous levels of total SRF protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
508
Applicable Applications for anti-SRF antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:5000
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human SRF.
Immunogen Range
71-120
Preparation and Storage
Stable at -20 degree C for at least 1 year.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using SRF Antibody. The picture on the right is blocked with the synthesized peptide.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using SRF Antibody. The picture on the right is blocked with the synthesized peptide.)

Western Blot (WB)

(Western blot analysis of lysates from NIH/3T3 cells, treated with PMA 125ng/ml 30', using SRF Antibody. The lane on the right is blocked with the synthesized peptide.)

Western Blot (WB) (Western blot analysis of lysates from NIH/3T3 cells, treated with PMA 125ng/ml 30', using SRF Antibody. The lane on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51 kDa
NCBI Official Full Name
serum response factor isoform 2
NCBI Official Synonym Full Names
serum response factor
NCBI Official Symbol
SRF
NCBI Official Synonym Symbols
MCM1
NCBI Protein Information
serum response factor
UniProt Protein Name
Serum response factor
Protein Family
UniProt Gene Name
SRF
UniProt Synonym Gene Names
SRF

NCBI Description

This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]

Uniprot Description

SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.

Research Articles on SRF

Similar Products

Product Notes

The SRF srf (Catalog #AAA9503508) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SRF Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SRF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000. Researchers should empirically determine the suitability of the SRF srf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SRF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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