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Western Blot (WB) (Western blot analysis of lysates from mouse NIH/3T3;rat C6 cell line (from left to right), using Srf Antibody (C-term). MBS9215248 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

Rabbit anti-Mouse, rat Srf Polyclonal Antibody | anti-Srf antibody

(Mouse) Srf Antibody (C-term)

Gene Names
Srf; AW049942; AW240594
Reactivity
Mouse, rat
Applications
Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Srf; Polyclonal Antibody; (Mouse) Srf Antibody (C-term); Serum response factor; SRF; anti-Srf antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse, rat
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This (Mouse) Srf antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 489-524 amino acids from the C-terminal region of human (Mouse) Srf.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
504
Applicable Applications for anti-Srf antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of lysates from mouse NIH/3T3;rat C6 cell line (from left to right), using Srf Antibody (C-term). MBS9215248 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

Western Blot (WB) (Western blot analysis of lysates from mouse NIH/3T3;rat C6 cell line (from left to right), using Srf Antibody (C-term). MBS9215248 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Related Product Information for anti-Srf antibody
SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS) (By similarity). Required for cardiac differentiation and maturation.
Product Categories/Family for anti-Srf antibody
References
Miwa T.,et al.Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases.
Zhang X.,et al.J. Biol. Chem. 279:55626-55632(2004).
Parlakian A.,et al.Mol. Cell. Biol. 24:5281-5289(2004).
Zhang X.,et al.Biochem. Biophys. Res. Commun. 346:794-801(2006).
Villen J.,et al.Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51247
NCBI Official Full Name
serum response factor
NCBI Official Synonym Full Names
serum response factor
NCBI Official Symbol
Srf
NCBI Official Synonym Symbols
AW049942; AW240594
NCBI Protein Information
serum response factor
UniProt Protein Name
Serum response factor
Protein Family
UniProt Gene Name
Srf
UniProt Synonym Gene Names
SRF
UniProt Entry Name
SRF_MOUSE

Uniprot Description

SRF: a transcription factor of the MADS domain family that binds to the serum response element (SRE). Regulates the transcription of immediate early genes including c-fos. Binds DNA as a multimer, probably a dimer.

Protein type: Transcription factor; Motility/polarity/chemotaxis

Cellular Component: cell; cytoplasm; nuclear chromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein dimerization activity; protein binding; protein homodimerization activity; DNA binding; chromatin DNA binding; sequence-specific DNA binding; histone deacetylase binding; chromatin binding; transcription factor binding; transcription factor activity

Biological Process: developmental growth; positive regulation of transcription, DNA-dependent; positive regulation of axon extension; heart development; multicellular organismal development; gastrulation; muscle maintenance; cell-cell adhesion; tangential migration from the subventricular zone to the olfactory bulb; morphogenesis of an epithelial sheet; neurite development; platelet activation; skin morphogenesis; transcription, DNA-dependent; leukocyte differentiation; actin filament organization; trophectodermal cell differentiation; regulation of water loss via skin; patterning of blood vessels; cell migration during sprouting angiogenesis; mesoderm formation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; positive regulation of cell differentiation; actin cytoskeleton organization and biogenesis; transcription from RNA polymerase II promoter; erythrocyte development; positive regulation of transcription by glucose; cell-matrix adhesion; response to hormone stimulus; neuron migration; cardiac myofibril assembly; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; heart looping; positive regulation of smooth muscle contraction; negative regulation of cell migration; associative learning; positive thymic T cell selection; positive regulation of filopodium formation; regulation of cell adhesion; in utero embryonic development; hippocampus development; sarcomere organization; mRNA transcription from RNA polymerase II promoter; contractile actin filament bundle formation; long-term memory; response to cytokine stimulus; stress fiber formation; platelet formation

Research Articles on Srf

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Product Notes

The Srf srf (Catalog #AAA9215248) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The (Mouse) Srf Antibody (C-term) reacts with Mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's Srf can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the Srf srf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Srf, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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