Rabbit anti-Human sRANKL Polyclonal Antibody
sRANKL (soluble RANK Ligand, Receptor Activator of NFkB Ligand) (Biotin)
Reactivity
Human
Applications
ELISA, Western Blot, Neutralization Assay
Purity
Affinity PurifiedPurified by antigen affinity chromatography.
Synonyms
sRANKL; Polyclonal Antibody; sRANKL (soluble RANK Ligand; Receptor Activator of NFkB Ligand) (Biotin); Anti -sRANKL (soluble RANK Ligand; anti-sRANKL antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes sRANKL
Purity/Purification
Affinity Purified
Purified by antigen affinity chromatography.
Purified by antigen affinity chromatography.
Form/Format
Supplied as a lyophilized powder from a sterile-filtered solution in PBS, pH 7.4, conjugated to biotin.
Applicable Applications for anti-sRANKL antibody
ELISA (EL/EIA), Western Blot (WB), Neutralization
Application Notes
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hsRANKL (30ng/ml), a concentration of 0.42-0.9ug/ml of this antibody is required.
ELISA: To detect hsRANKL by direct ELISA (using 100ul/well antibody solution) a concentration of at least 0.5ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4ng/well of recombinant hsRANKL.
Western Blot: To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
ELISA: To detect hsRANKL by direct ELISA (using 100ul/well antibody solution) a concentration of at least 0.5ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4ng/well of recombinant hsRANKL.
Western Blot: To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Immunogen
Highly purified, recombinant sRANKL
Country of Origin
USA
Reconstitution
The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at- 20 degree C. When reconstituted in sterile water to a concentration of 0.2mg/ml the antibody is stable for at least six weeks at 2-4 degree C.
Preparation and Storage
-20 degree C
Product Categories/Family for anti-sRANKL antibody
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Product Notes
The sRANKL (Catalog #AAA610170) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The sRANKL (soluble RANK Ligand, Receptor Activator of NFkB Ligand) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's sRANKL can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Neutralization. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hsRANKL (30ng/ml), a concentration of 0.42-0.9ug/ml of this antibody is required. ELISA: To detect hsRANKL by direct ELISA (using 100ul/well antibody solution) a concentration of at least 0.5ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4ng/well of recombinant hsRANKL. Western Blot: To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Researchers should empirically determine the suitability of the sRANKL for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "sRANKL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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