Western Blot (WB)
(SPT19 Antibody (C-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the SPT19 antibody detected the SPT19 protein (arrow).)
Rabbit anti-Mouse SPT19 Polyclonal Antibody | anti-SPT19 antibody
SPT19, CT (SPATA19, SPERGEN1, Spermatogenesis-associated protein 19, mitochondrial, Spermatogenic cell-specific gene 1 protein) (PE)
Gene Names
SPATA19; CT132; SPAS1; spergen1
Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
SPT19; Polyclonal Antibody; CT (SPATA19; SPERGEN1; Spermatogenesis-associated protein 19; mitochondrial; Spermatogenic cell-specific gene 1 protein) (PE); SPATA19; Spermatogenic cell-specific gene 1 protein; anti-SPT19 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-SPT19 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
SPT19 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-150 amino acids from the C-terminal region of human SPT19.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(SPT19 Antibody (C-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the SPT19 antibody detected the SPT19 protein (arrow).)
Western Blot (WB)
(SPT19 Antibody (C-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the SPT19 antibody detected the SPT19 protein (arrow).)
Immunohistochemistry (IHC)
(SPT19 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SPT19 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(SPT19 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SPT19 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Product Categories/Family for anti-SPT19 antibody
References
Ghafouri-Fard, S., et al. Br. J. Dermatol. 162(4):772-779(2010). Stolk, L., et al. Nat. Genet. 41(6):645-647(2009). Lamesch, P., et al. Genomics 89(3):307-315(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,186 Da
NCBI Official Full Name
spermatogenesis-associated protein 19, mitochondrial
NCBI Official Synonym Full Names
spermatogenesis associated 19
NCBI Official Symbol
SPATA19
NCBI Official Synonym Symbols
CT132; SPAS1; spergen1
NCBI Protein Information
spermatogenesis-associated protein 19, mitochondrial; spergen-1; cancer/testis antigen 132; spermatogenic specific-gene1; spermatogenic cell-specific gene 1 protein
UniProt Protein Name
Spermatogenesis-associated protein 19, mitochondrial
UniProt Gene Name
SPATA19
UniProt Synonym Gene Names
SPERGEN1; Spergen-1
UniProt Entry Name
SPT19_HUMAN
Uniprot Description
spergen-1: May have a role in spermiogenesis.
Protein type: Cancer Testis Antigen (CTA)
Chromosomal Location of Human Ortholog: 11q25
Cellular Component: mitochondrial outer membrane
Biological Process: multicellular organismal development; spermatogenesis; cell differentiation
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Product Notes
The SPT19 spata19 (Catalog #AAA6350937) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPT19, CT (SPATA19, SPERGEN1, Spermatogenesis-associated protein 19, mitochondrial, Spermatogenic cell-specific gene 1 protein) (PE) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SPT19 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SPT19 spata19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPT19, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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