Western Blot (WB)
(Western blot analysis of K562 A375 and 231 cell using SPRY4 Polyclonal Antibody at dilution of 1:400)
Rabbit anti-Human, Mouse SPRY4 Polyclonal Antibody | anti-SPRY4 antibody
SPRY4 Polyclonal Antibody
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Antigen Affinity Purification
Synonyms
SPRY4; Polyclonal Antibody; SPRY4 Polyclonal Antibody; Protein sprouty homolog 4; Sprouty homolog 4; Sprouty homolog 4 (Drosophila); SPRY 4; Spry-4; Spry4; SPY4; anti-SPRY4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purification
Form/Format
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Concentration
0.8mg/mL (varies by lot)
Sequence Length
299
Applicable Applications for anti-SPRY4 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:25-1:100
ELISA: 1:5000-1:10000
IHC: 1:25-1:100
ELISA: 1:5000-1:10000
Immunogen
Full length fusion protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of K562 A375 and 231 cell using SPRY4 Polyclonal Antibody at dilution of 1:400)
Western Blot (WB)
(Western blot analysis of K562 A375 and 231 cell using SPRY4 Polyclonal Antibody at dilution of 1:400)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human lung cancer tissue using SPRY4 Polyclonal Antibody at dilution of 1:25(×200))
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human lung cancer tissue using SPRY4 Polyclonal Antibody at dilution of 1:25(×200))
Related Product Information for anti-SPRY4 antibody
This gene encodes a member of a family of cysteine- and proline-rich proteins. The encoded protein is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. Activity of this protein impairs the formation of active GTP-RAS. Nucleotide variation in this gene has been associated with hypogonadotropic hypogonadism 17 with or without anosmia. Alternative splicing results in a multiple transcript variants.
Product Categories/Family for anti-SPRY4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33 kDa
NCBI Official Full Name
protein sprouty homolog 4 isoform 2
UniProt Protein Name
Protein sprouty homolog 4
Protein Family
UniProt Gene Name
SPRY4
UniProt Synonym Gene Names
Spry-4
UniProt Entry Name
SPY4_HUMAN
Uniprot Description
SPRY4: Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. Inhibits Ras-independent, but not Ras-dependent, activation of RAF1. Belongs to the sprouty family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold; Inhibitor
Chromosomal Location of Human Ortholog: 5q31.3
Cellular Component: Golgi apparatus; focal adhesion; cytoplasm
Molecular Function: protein binding
Biological Process: negative regulation of MAP kinase activity; multicellular organismal development
Disease: Hypogonadotropic Hypogonadism 17 With Or Without Anosmia
Similar Products
Product Notes
The SPRY4 spry4 (Catalog #AAA2562122) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPRY4 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SPRY4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:25-1:100 ELISA: 1:5000-1:10000. Researchers should empirically determine the suitability of the SPRY4 spry4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPRY4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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