Rabbit anti-Human SPRY2 Polyclonal Antibody | anti-SPRY2 antibody

SPRY2 (Protein Sprouty Homolog 2, Spry-2, hSPRY2) (HRP)

Cat. Num. AAA6395019

• Gene Names: SPRY2; IGAN3; hSPRY2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SPRY2; Polyclonal Antibody; SPRY2 (Protein Sprouty Homolog 2; Spry-2; hSPRY2) (HRP); anti-SPRY2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SPRY2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 2286

• Applicable Applications for anti-SPRY2 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full-length human SPRY2, aa1-315 (NP_005833.1).
• Immunogen Sequence: MEARAQSGNGSQPLLQTPRDGGRQRGEPDPRDALTQQVHVLSLDQIRAIRNTNEYTEGPTVVPRPGLKPAPRPSTQHKHERLHGLPEHRQPPRLQHSQVHSSARAPLSRSISTVSSGSRSSTRTSTSSSSSEQRLLGSSFSSGPVADGIIRVQPKSELKPGELKPLSKEDLGLHAYRCEDCGKCKCKECTYPRPLPSDWICDKQCLCSAQNVIDYGTCVCCVKGLFYHCSNDDEDNCADNPCSCSQSHCCTRWSAMGVMSLFLPCLWCYLPAKGCLKLCQGCYDRVNRPGCRCKNSNTVCCKVPTVPPRNFEKPT
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SPRY2 rabbit polyclonal antibody. Western Blot analysis of SPRY2 expression in HeLa.)

Western Blot (WB)

(Western Blot analysis of SPRY2 expression in transfected 293T cell line by SPRY2 polyclonal antibody. Lane 1: SPRY2 transfected lysate (34.7kD). Lane 2: Non-transfected lysate.)

Product Categories/Family for anti-SPRY2 antibody

Antibodies; Sprouty

NCBI and Uniprot Product Information

• NCBI GI #: 1519243998
• NCBI GeneID: 10253
• NCBI Accession #: NM_005842.4
• NCBI GenBank Nucleotide #: NM_005842
• UniProt Accession #: O43597
• NCBI Official Full Name: Homo sapiens sprouty RTK signaling antagonist 2 (SPRY2), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: sprouty RTK signaling antagonist 2
• NCBI Official Symbol: SPRY2
• NCBI Official Synonym Symbols: IGAN3; hSPRY2
• NCBI Protein Information: protein sprouty homolog 2
• UniProt Protein Name: Protein sprouty homolog 2
• Protein Family: Protein sprouty
• UniProt Gene Name: SPRY2
• UniProt Synonym Gene Names: Spry-2
• UniProt Entry Name: SPY2_HUMAN

NCBI Description

This gene encodes a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. This protein is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. This protein may play a role in alveoli branching during lung development as shown by a similar mouse protein. [provided by RefSeq, Jul 2008]

Uniprot Description

SPRY2: a feedback inhibitor of growth factor receptor signaling. Belongs to the sprouty family. May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis. Associated with microtubules in unstimulated cells but is translocated to the membrane ruffles in cells stimulated with EGF (epidermal growth factor).

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis; Inhibitor

Chromosomal Location of Human Ortholog: 13q31.1

Cellular Component: microtubule cytoskeleton; microtubule; plasma membrane; cytosol

Molecular Function: protein serine/threonine kinase inhibitor activity; protein serine/threonine kinase activator activity; protein binding; protein kinase binding

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of MAP kinase activity; inner ear morphogenesis; cell fate commitment; fibroblast growth factor receptor signaling pathway; negative regulation of fibroblast growth factor receptor signaling pathway; negative regulation of nerve growth factor receptor signaling pathway; positive regulation of peptidyl-serine phosphorylation; negative regulation of cell proliferation; positive regulation of protein kinase B signaling cascade; sensory perception of sound; establishment of mitotic spindle orientation; negative regulation of Ras protein signal transduction; negative regulation of apoptosis