Western Blot (WB)
(Anti-splicing factor 1 antibody, MBS176161, Western blottingAll lanes: Anti splicing factor 1 (MBS176161) at 0.5ug/mlLane 1: Rat Spleen Tissue Lysate at 50ugLane 2: Rat Liver Tissue Lysate at 50ugLane 3: PANC Whole Cell Lysate at 40ugLane 4: COLO320 Whole Cell Lysate at 40ugLane 5: SW620 Whole Cell Lysate at 40ugLane 6: SKOV Whole Cell Lysate at 40ugPredicted bind size: 52KDObserved bind size: 70KD)
Rabbit splicing factor 1 Polyclonal Antibody | anti-SF1 antibody
Anti-splicing factor 1 antibody
Western Blot (WB)
(Anti-splicing factor 1 antibody, MBS176161, Western blottingAll lanes: Anti splicing factor 1 (MBS176161) at 0.5ug/mlLane 1: Rat Spleen Tissue Lysate at 50ugLane 2: Rat Liver Tissue Lysate at 50ugLane 3: PANC Whole Cell Lysate at 40ugLane 4: COLO320 Whole Cell Lysate at 40ugLane 5: SW620 Whole Cell Lysate at 40ugLane 6: SKOV Whole Cell Lysate at 40ugPredicted bind size: 52KDObserved bind size: 70KD)
Western Blot (WB)
(Anti-splicing factor 1 antibody, MBS176161, Western blottingAll lanes: Anti splicing factor 1 (MBS176161) at 0.5ug/mlLane 1: Rat Spleen Tissue Lysate at 50ugLane 2: Rat Liver Tissue Lysate at 50ugLane 3: PANC Whole Cell Lysate at 40ugLane 4: COLO320 Whole Cell Lysate at 40ugLane 5: SW620 Whole Cell Lysate at 40ugLane 6: SKOV Whole Cell Lysate at 40ugPredicted bind size: 52KDObserved bind size: 70KD)
Immunohistochemistry (IHC)
(Anti-splicing factor 1 antibody, MBS176161, IHC(P)IHC(P): Human Lung Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-splicing factor 1 antibody, MBS176161, IHC(P)IHC(P): Human Lung Cancer Tissue )
Immunocytochemistry (ICC)
(Anti-splicing factor 1 antibody, MBS176161, ICCICC: HELA Cell )
Immunocytochemistry (ICC)
(Anti-splicing factor 1 antibody, MBS176161, ICCICC: HELA Cell )
Immunohistochemistry (IHC)
(Anti-splicing factor 1 antibody, MBS176161, IHC(F)IHC(F): Rat Spleen Tissue )
Immunohistochemistry (IHC)
(Anti-splicing factor 1 antibody, MBS176161, IHC(F)IHC(F): Rat Spleen Tissue )
Background: SF1(splicing factor 1), also known as zinc finger protein 162(ZFM162), ZNF162, Transcription factor ZFM1, ZFM1, D11S636, Mammalian branch point-binding protein(BBP, mBBP), Zinc finger gene in MEN1 locus, is a protein that in humans is encoded by the SF1 gene. It is predicted 623-amino acid protein. RT-PCR was used to show expression in the thyroid gland, pancreas, adrenal gland, and ovary. All of the ZNF162 isoforms contain a KH domain, a sequence motif present in proteins playinig a major role in regulating cellular RNA metabolism.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence and is required for the early stages of spliceosome assembly. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]
Uniprot Description
SF1: a splicing factor necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor. Phosphorylation of SF1 by cGMP-dependent protein kinase regulates spliceosome assembly. Binds U2AF2. Interacts with U1 snRNA. Binds EWSR1, FUS and TAF15. Six alternatively spliced isoforms have been described.
Protein type: Transcription, coactivator/corepressor; Spliceosome; RNA splicing; RNA-binding
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: nucleoplasm; spliceosome; ribosome; nucleus
Molecular Function: zinc ion binding; RNA binding; transcription corepressor activity
Biological Process: nuclear mRNA 3'-splice site recognition; nuclear mRNA splicing, via spliceosome; Leydig cell differentiation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; negative regulation of smooth muscle cell proliferation; male sex determination; spliceosome assembly; regulation of steroid biosynthetic process
Research Articles on SF1
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Product Notes
The SF1 sf1 (Catalog #AAA176161) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-splicing factor 1 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's splicing factor 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry ICC. Researchers should empirically determine the suitability of the SF1 sf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "splicing factor 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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