Testing Data
(Gel: 12%SDS-PAGE Lysate: 40ug SP20 cell Primary antibody: 1/200 dilution Secondary antibody dilution: 1/8000 Exposure time: 1 minute)
Rabbit anti-Human, Mouse SPIN2B Polyclonal Antibody | anti-SPIN2B antibody
SPIN2B Antibody
Gene Names
SPIN2B; SPIN-2; SPIN-2B; dJ323P24.2; SPIN2_duplicate
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification.
Synonyms
SPIN2B; Polyclonal Antibody; SPIN2B Antibody; SPIN-2; dJ323P24.2; SPIN2_duplicate; anti-SPIN2B antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total SPIN2B protein.
Purity/Purification
Antigen affinity purification.
Form/Format
Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
Concentration
1.1 mg/ml (varies by lot)
Sequence Length
258
Applicable Applications for anti-SPIN2B antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western blotting: 1:200-1:1000
Immunohistochemistry: 1:25-1:100
Immunohistochemistry: 1:25-1:100
Immunogen Type
Recombinant Protein
Immunogen Description
Full length fusion protein
Target Name
SPIN2B
Preparation and Storage
Store at -20 degree C
Testing Data
(Gel: 12%SDS-PAGE Lysate: 40ug SP20 cell Primary antibody: 1/200 dilution Secondary antibody dilution: 1/8000 Exposure time: 1 minute)
Testing Data
(Gel: 12%SDS-PAGE Lysate: 40ug SP20 cell Primary antibody: 1/200 dilution Secondary antibody dilution: 1/8000 Exposure time: 1 minute)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue using at dilution 1/25.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue using at dilution 1/25.)
Related Product Information for anti-SPIN2B antibody
Human SPIN2 expressed by 2 IL3-dependent murine myeloid cell lines was moderately antiapoptotic following IL3 removal, but it was unable to block apoptosis induced by the chemotherapy agent doxorubicin. On the contrary, SPIN2-expressing cells were more sensitive to doxorubicin-mediated cell death. Also, SPIN2 did not combat the proapoptotic effects of Fas ligand stimulation in the Jurkat human T-cell line. Deletion of the C-terminal amino acids of SPIN2 diminished its antiapoptotic activity. In the murine myeloid cells, SPIN2 overexpression increased the cell number doubling times and slowed their rate of growth. There was also an increased percentage of cells in G2/M, which was more pronounced following IL3 withdrawal.
Product Categories/Family for anti-SPIN2B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,158 Da
NCBI Official Full Name
spindlin-2B isoform 1
NCBI Official Synonym Full Names
spindlin family, member 2B
NCBI Official Symbol
SPIN2B
NCBI Official Synonym Symbols
SPIN-2; SPIN-2B; dJ323P24.2; SPIN2_duplicate
NCBI Protein Information
spindlin-2B
UniProt Protein Name
Spindlin-2B
Protein Family
UniProt Gene Name
SPIN2B
UniProt Synonym Gene Names
SPIN2; SPIN-2; SPIN-2B
UniProt Entry Name
SPI2B_HUMAN
Uniprot Description
SPIN2B: Involved in the regulation of cell cycle progression, this activity is related to the inhibition of apoptosis following the removal of essential growth factors. Belongs to the SPIN/STSY family.
Chromosomal Location of Human Ortholog: Xp11.1
Cellular Component: nucleus
Biological Process: regulation of cell cycle; apoptosis; gamete generation; cell cycle
Research Articles on SPIN2B
Similar Products
Product Notes
The SPIN2B spin2b (Catalog #AAA9402118) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPIN2B Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SPIN2B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western blotting: 1:200-1:1000 Immunohistochemistry: 1:25-1:100. Researchers should empirically determine the suitability of the SPIN2B spin2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPIN2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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