Rabbit SPG20 Polyclonal Antibody | anti-SPG20 antibody

SPG20 Antibody (N-term)

Cat. Num. AAA9201810

• Gene Names: SPG20; SPARTIN; TAHCCP1
• Reactivity: Human (Predicted Reactivity: Bovine, Mouse)
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: SPG20; Polyclonal Antibody; SPG20 Antibody (N-term); Spartin; Spastic paraplegia 20 protein; Trans-activated by hepatitis C virus core protein 1; KIAA0610; TAHCCP1; anti-SPG20 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Bovine, Mouse)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This SPG20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-45 amino acids from the N-terminal region of human SPG20.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.3 (varies by lot)
• Sequence Positions: 18-45
• Sequence Length: 666

• Applicable Applications for anti-SPG20 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of SPG20 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). SPG20 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with SPG20 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(SPG20 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-SPG20 antibody

SPG20 is a protein containing a MIT (Microtubule Interacting and Trafficking molecule) domain, and is implicated in regulating endosomal trafficking and mitochondria function. The protein localizes to mitochondria and partially co-localizes with microtubules. Stimulation with epidermal growth factor (EGF) results in protein translocation to the plasma membrane, and the protein functions in the degradation and intracellular trafficking of EGF receptor.

References

Milewska,M., et.al., J. Neurochem. 111 (4), 1022-1030 (2009);
Edwards,T.L., et.al., Biochem. J. 423 (1), 31-39 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 214830079
• NCBI GeneID: 23111
• NCBI Accession #: NP_001135766.1; NP_001135767.1; NP_001135768.1; NP_055902.1
• NCBI GenBank Nucleotide #: NM_001142294.1
• UniProt Accession #: Q8N0X7; O60349; Q86Y67; Q9H1T2; Q9H1T3
• Molecular Weight: 72833
• NCBI Official Full Name: spartin
• NCBI Official Synonym Full Names: spastic paraplegia 20 (Troyer syndrome)
• NCBI Official Symbol: SPG20
• NCBI Official Synonym Symbols: SPARTIN; TAHCCP1
• NCBI Protein Information: spartin
• UniProt Protein Name: Spartin
• Protein Family: Spartin
• UniProt Gene Name: SPG20
• UniProt Synonym Gene Names: KIAA0610; TAHCCP1
• UniProt Entry Name: SPG20_HUMAN

NCBI Description

This gene encodes a protein containing a MIT (Microtubule Interacting and Trafficking molecule) domain, and is implicated in regulating endosomal trafficking and mitochondria function. The protein localizes to mitochondria and partially co-localizes with microtubules. Stimulation with epidermal growth factor (EGF) results in protein translocation to the plasma membrane, and the protein functions in the degradation and intracellular trafficking of EGF receptor. Multiple alternatively spliced variants, encoding the same protein, have been identified. Mutations associated with this gene cause autosomal recessive spastic paraplegia 20 (Troyer syndrome). [provided by RefSeq, Nov 2008]

Uniprot Description

SPG20: May be implicated in endosomal trafficking, or microtubule dynamics, or both. Defects in SPG20 are the cause of spastic paraplegia autosomal recessive type 20 (SPG20); also known as Troyer syndrome (TRS). Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. SPG20 is characterized by dysarthria, distal amyotrophy, mild developmental delay and short stature.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 13q13.3

Cellular Component: mitochondrial outer membrane; cytoplasm; plasma membrane; lipid particle; synapse; midbody

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: negative regulation of collateral sprouting in the absence of injury; regulation of mitochondrial membrane potential; cell division; neuromuscular process; abscission; negative regulation of BMP signaling pathway

Disease: Spastic Paraplegia 20, Autosomal Recessive