Western Blot (WB)
(Western Blot analysis of SPESP1 expression in transfected 293T cell line by SPESP1 polyclonal antibody. Lane 1: SPESP1 transfected lysate (38.5kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human SPESP1 Polyclonal Antibody | anti-Spesp1 antibody
SPESP1 (Sperm Equatorial Segment Protein 1, SP-ESP, Equatorial Segment Protein, ESP, Glycosylated 38 kDa Sperm Protein C-7/8, UNQ732/PRO1418)
Gene Names
Spesp1; AI428149; 4921508E09Rik
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
SPESP1; Polyclonal Antibody; SPESP1 (Sperm Equatorial Segment Protein 1; SP-ESP; Equatorial Segment Protein; ESP; Glycosylated 38 kDa Sperm Protein C-7/8; UNQ732/PRO1418); Anti -SPESP1 (Sperm Equatorial Segment Protein 1; anti-Spesp1 antibody
Host
Mouse
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human SPESP1.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
MKPLVLLVALLLWPSSVPAYPSITVTPDEEQNLNHYIQVLENLVRSVPSGEPGREKKSNSPKHVYSIASKGSKFKELVTHGDASTENDVLTNPISEETTTFPTGGFTPEIGKKKHTESTPFWSIKPNNVSIVLHAEEPYIENEEPEPEPEPAAKQTEAPRMLPVVTESSTSPYVTSYKSPVTTLDKSTGIGISTESEDVPQLSGETAIEKPEEFGKHPESWNNDDILKKILDINSQVQQALLSDTSNPAYREDIEASKDHLKRSLALAAAAEHKLKTMYKSQLLPVGRTSNKIDDIETVINMLCNSRSKLYEYLDIKCVPPEMREKAATVFNTLKNMCRSRRVTALLKVY
Applicable Applications for anti-Spesp1 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Immunogen
Full length human SPESP1, aa1-350 (NP_663633.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of SPESP1 expression in transfected 293T cell line by SPESP1 polyclonal antibody. Lane 1: SPESP1 transfected lysate (38.5kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of SPESP1 expression in transfected 293T cell line by SPESP1 polyclonal antibody. Lane 1: SPESP1 transfected lysate (38.5kD). Lane 2: Non-transfected lysate.)
Product Categories/Family for anti-Spesp1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,702 Da
NCBI Official Full Name
sperm equatorial segment protein 1
NCBI Official Synonym Full Names
sperm equatorial segment protein 1
NCBI Official Symbol
Spesp1
NCBI Official Synonym Symbols
AI428149; 4921508E09Rik
NCBI Protein Information
sperm equatorial segment protein 1
UniProt Protein Name
Sperm equatorial segment protein 1
Protein Family
UniProt Gene Name
Spesp1
UniProt Entry Name
SPESP_MOUSE
Uniprot Description
SPESP1: The encoded protein is a human alloantigen involved in sperm-egg binding and fusion. [provided by RefSeq, Apr 2010]
Protein type: Secreted, signal peptide; Cytoskeletal; Secreted
Cellular Component: cell; acrosome; cytoplasmic vesicle
Molecular Function: protein binding
Biological Process: fusion of sperm to egg plasma membrane; multicellular organismal development; acrosome reaction
Research Articles on Spesp1
Similar Products
Product Notes
The Spesp1 spesp1 (Catalog #AAA642986) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SPESP1 (Sperm Equatorial Segment Protein 1, SP-ESP, Equatorial Segment Protein, ESP, Glycosylated 38 kDa Sperm Protein C-7/8, UNQ732/PRO1418) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SPESP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the Spesp1 spesp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MKPLVLLVAL LLWPSSVPAY PSITVTPDEE QNLNHYIQVL ENLVRSVPSG EPGREKKSNS PKHVYSIASK GSKFKELVTH GDASTENDVL TNPISEETTT FPTGGFTPEI GKKKHTESTP FWSIKPNNVS IVLHAEEPYI ENEEPEPEPE PAAKQTEAPR MLPVVTESST SPYVTSYKSP VTTLDKSTGI GISTESEDVP QLSGETAIEK PEEFGKHPES WNNDDILKKI LDINSQVQQA LLSDTSNPAY REDIEASKDH LKRSLALAAA AEHKLKTMYK SQLLPVGRTS NKIDDIETVI NMLCNSRSKL YEYLDIKCVP PEMREKAATV FNTLKNMCRS RRVTALLKVY. It is sometimes possible for the material contained within the vial of "SPESP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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