Rabbit anti-Human, Mouse SPEN Polyclonal Antibody | anti-SPEN antibody

SPEN Antibody

Cat. Num. AAA9631025

• Gene Names: SPEN; MINT; SHARP; RBM15C; HIAA0929
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: SPEN; Polyclonal Antibody; SPEN Antibody; MINT; Nuclear receptor transcription cofactor; RBM15C; SHARP; SPEN homolog; Spen homolog transcriptional regulator; anti-SPEN antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: SPEN Antibody detects endogenous levels of SPEN.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-SPEN antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human SPEN.
• Conjugation: Unconjugated
• Similarity: The RID domain mediates the interaction with nuclear receptors. The SPOC domain, which mediates the interaction with NCOR2, is essential for the repressive activity. Belongs to the RRM Spen family.
• Subunit Structure: Interacts with MSX2 and HIPK3 (By similarity). Interacts with NCOR2, HDAC1, HDAC2, RBBP4, MBD3 and MTA1L1. Interacts with RBPSUH; this interaction may prevent the interaction between RBPSUH and NOTCH1. Interacts with the nuclear receptors RAR and PPARD. Interacts with RAR in absence of ligand. Binds to the steroid receptor RNA coactivator SRA.(Microbial infection) Interacts with Epstein-Barr virus BSFL2/BMLF1.
• Tissue Specificity: Expressed at high level in brain, testis, spleen and thymus. Expressed at intermediate level in kidney, liver, mammary gland and skin.
• Function: May serve as a nuclear matrix platform that organizes and integrates transcriptional responses. In osteoblasts, supports transcription activation: synergizes with RUNX2 to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Has also been shown to be an essential corepressor protein, which probably regulates different key pathways such as the Notch pathway. Negative regulator of the Notch pathway via its interaction with RBPSUH, which prevents the association between NOTCH1 and RBPSUH, and therefore suppresses the transactivation activity of Notch signaling. Blocks the differentiation of precursor B-cells into marginal zone B-cells. Probably represses transcription via the recruitment of large complexes containing histone deacetylase proteins. May bind both to DNA and RNA.
• Note: Associates with chromatin.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-SPEN antibody

Nucleus; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 14790190
• NCBI GeneID: 23013
• NCBI Accession #: NP_055816.2
• NCBI GenBank Nucleotide #: NM_015001.2
• UniProt Accession #: Q96T58; Q9H9A8; Q9NWH5; Q9UQ01; Q9Y556
• Molecular Weight: 402,248 Da
• NCBI Official Full Name: msx2-interacting protein
• NCBI Official Synonym Full Names: spen family transcriptional repressor
• NCBI Official Symbol: SPEN
• NCBI Official Synonym Symbols: MINT; SHARP; RBM15C; HIAA0929
• NCBI Protein Information: msx2-interacting protein
• UniProt Protein Name: Msx2-interacting protein
• Protein Family: Protein split ends
• UniProt Gene Name: SPEN
• UniProt Synonym Gene Names: KIAA0929; MINT; SHARP
• UniProt Entry Name: MINT_HUMAN

NCBI Description

This gene encodes a hormone inducible transcriptional repressor. Repression of transcription by this gene product can occur through interactions with other repressors, by the recruitment of proteins involved in histone deacetylation, or through sequestration of transcriptional activators. The product of this gene contains a carboxy-terminal domain that permits binding to other corepressor proteins. This domain also permits interaction with members of the NuRD complex, a nucleosome remodeling protein complex that contains deacetylase activity. In addition, this repressor contains several RNA recognition motifs that confer binding to a steroid receptor RNA coactivator; this binding can modulate the activity of both liganded and nonliganded steroid receptors. [provided by RefSeq, Jul 2008]