SP3 Polyclonal Antibody | anti-SP3 antibody

Anti-SP3 Antibody

Cat. Num. AAA178186

• Gene Names: SP3; SPR2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: SP3; Polyclonal Antibody; Anti-SP3 Antibody; Transcription factor Sp3; D130027J01Rik; DKFZp686O1631; GC binding transcription factor Sp 3; GC binding transcription factor Sp3; MGC105187; OTTMUSP00000014207; SP 3; Sp 3 transcription factor; Sp3 transcription factor; SP3_HUMAN; Specificity protein 3; SPR 2; SPR-2; SPR2; Transcription factor SP 3; anti-SP3 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 713

• Applicable Applications for anti-SP3 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human SP3 (569-599aa NTNDLTHLRVQVVDEEGDQQHQEGKRLRRVA), identical to the related mouse sequence.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- SP3 Picoband antibody, MBS178186, Western blottingAll lanes: Anti SP3 (MBS178186) at 0.5ug/mlLane 1: PC-12 Whole Cell Lysate at 40ugLane 2: HEPA Whole Cell Lysate at 40ugLane 3: HELA Whole Cell Lysate at 40ugPredicted bind size: 82KDObserved bind size: 82KD )

Immunohistochemistry (IHC)

(Anti- SP3 Picoband antibody, MBS178186, IHC(P)IHC(P): Mouse Lung Tissue)

Immunohistochemistry (IHC)

(Anti- SP3 Picoband antibody, MBS178186, IHC(P)IHC(P): Rat Lung Tissue)

Immunohistochemistry (IHC)

(Anti- SP3 Picoband antibody, MBS178186, IHC(P)IHC(P): Human Intestinal Cancer Tissue )

Related Product Information for anti-SP3 antibody

Description: Rabbit IgG polyclonal antibody for Transcription factor Sp3(SP3) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Background: Sp3 transcription factor, also known as SP3, refers to both a protein and the gene it is encoded by. This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. And this protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13.

References

1. "Entrez Gene: SP3 Sp3 transcription factor". 2. Sun, Jian-Min; Chen Hou Yu; Moniwa Mariko; Litchfield David W; Seto Edward; Davie James R (September 2002). "The transcriptional repressor Sp3 is associated with CK2-phosphorylated histone deacetylase 2". J. Biol. Chem.(United States) 277 (39): 35783-6.

NCBI and Uniprot Product Information

• NCBI GI #: 289577125
• NCBI GeneID: 6670
• NCBI Accession #: NP_001017371.3
• NCBI GenBank Nucleotide #: NM_001017371.4
• UniProt Accession #: Q02447; Q69B26; Q69B27; Q8TD56; Q8WWU4; Q9BQR1; A0AVL9; B4E2B7
• Molecular Weight: 76,814 Da
• NCBI Official Full Name: transcription factor Sp3 isoform 2
• NCBI Official Synonym Full Names: Sp3 transcription factor
• NCBI Official Symbol: SP3
• NCBI Official Synonym Symbols: SPR2
• NCBI Protein Information: transcription factor Sp3
• UniProt Protein Name: Transcription factor Sp3
• Protein Family: Transcription factor
• UniProt Gene Name: SP3
• UniProt Entry Name: SP3_HUMAN

NCBI Description

This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13. [provided by RefSeq, Feb 2010]

Uniprot Description

Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone-induction and house-keeping.