Western Blot (WB)
(Western blot analysis of lysates from HeLa,Daudi cell line (from left to right),using SP3/4 Antibody(MBS9213842). MBS9213842 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.)
Rabbit SP3/4 Polyclonal Antibody | anti-SP3 antibody
SP3/4 Antibody
Western Blot (WB)
(Western blot analysis of lysates from HeLa,Daudi cell line (from left to right),using SP3/4 Antibody(MBS9213842). MBS9213842 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from HeLa,Daudi cell line (from left to right),using SP3/4 Antibody(MBS9213842). MBS9213842 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.)
Moran K.M.,et al.Gene 341:235-247(2004).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Oleksiak M.F.,et al.Mol. Biol. Evol. 19:2026-2029(2002).
Meyer-Grahle U.,et al.Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13. [provided by RefSeq, Feb 2010]
Uniprot Description
SP3: Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone- induction and house-keeping. Belongs to the Sp1 C2H2-type zinc-finger protein family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: C2H2-type zinc finger protein; DNA-binding
Chromosomal Location of Human Ortholog: 2q31
Cellular Component: nucleoplasm; Golgi apparatus; PML body; transcriptional repressor complex; cytoplasm; plasma membrane; nucleus
Molecular Function: protein binding; metal ion binding; double-stranded DNA binding; chromatin binding
Biological Process: transcription from RNA polymerase II promoter; embryonic placenta development; ossification; monocyte differentiation; granulocyte differentiation; megakaryocyte differentiation; embryonic skeletal development; positive regulation of transcription, DNA-dependent; embryonic process involved in female pregnancy; negative regulation of transcription from RNA polymerase II promoter; enucleate erythrocyte differentiation; liver development; trophectodermal cell differentiation; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; natural killer cell differentiation; B cell differentiation; embryonic camera-type eye morphogenesis; negative regulation of transcription, DNA-dependent; T cell differentiation; lung development
Research Articles on SP3
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Product Notes
The SP3 sp3 (Catalog #AAA9213842) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 655-683. The SP3/4 Antibody reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's SP3/4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the SP3 sp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SP3/4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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