Highly validated and characterized monoclonal/polyclonal
antibodies and recombinant
proteins
The majority of AAA Biotech’s antibodies are highly validated and can be use in multiple
applications such as ELISA, FC,
ICC, IF, IHC, IP, WB, etc. We have antibodies available for rare species, in multiple conjugated
forms or recombinant
antibodies.
As for our high quality proteins, the majority have 90% purity, detected by SDS-PAGE while some are
available in
different tags such as Flag, GST, His, MBP, etc. We also carry high quality native and biologically
active proteins.
AAA Biotech is constantly working to expand our capacity to provide recombinant proteins and
antibodies to most
target proteins.
SELECT `p`.*, `pd`.*, IFNULL(pdns.ncbi_summary, "N/A") as ncbi_summary_pdns, IFNULL(pdns.sp_comments, "N/A") as sp_comments_pdns, IFNULL(pdns.ncbi_research_articles, "N/A") as ncbi_research_articles_pdns, IFNULL(pe.products_description_extra, "N/A") as products_description_extra
FROM (`products`, `products` as `p`)
LEFT OUTER JOIN `products_description` as `pd` ON `p`.`products_id` = `pd`.`products_id`
LEFT OUTER JOIN `products_description_ncbi_sp` as `pdns` ON `p`.`products_id` = `pdns`.`products_id`
LEFT OUTER JOIN `products_extra` as `pe` ON `p`.`products_id` = `pe`.`products_id`
WHERE `p`.`products_id` = '30144'
AND `pd`.`language_id` = 1
LIMIT 1
Query
Database
2.36 ms
select p.*, pd.*,
ifnull(pdns.ncbi_summary, 'N/A') as ncbi_summary_pdns,
ifnull(pdns.sp_comments, 'N/A') as sp_comments_pdns,
ifnull(pdns.ncbi_research_articles, 'N/A') as ncbi_research_articles_pdns,
ifnull(pe.products_description_extra, 'N/A') as products_description_extra
from products p
LEFT OUTER JOIN products_description pd on p.products_id = pd.products_id
LEFT OUTER JOIN products_description_ncbi_sp pdns on p.products_id = pdns.products_id
LEFT OUTER JOIN products_extra pe on p.products_id = pe.products_id
where p.products_id = '30144' and pd.language_id = 1
Query
Database
2.34 ms
SELECT `options_values_price` as `price`, `products_options_values_name` as `package`
FROM `products_attributes`
JOIN `products_options_values` ON `products_options_values`.`products_options_values_id` = `products_attributes`.`options_values_id`
WHERE `products_attributes`.`products_id` = '30144'
Database (4 total Queries, 4 of them unique across 2 Connections)
Time
Query String
2.43 ms
SELECT `p`.*, `pd`.*, IFNULL(pdns.ncbi_summary, "N/A") as ncbi_summary_pdns, IFNULL(pdns.sp_comments, "N/A") as sp_comments_pdns, IFNULL(pdns.ncbi_research_articles, "N/A") as ncbi_research_articles_pdns, IFNULL(pe.products_description_extra, "N/A") as products_description_extra
FROM (`products`, `products` as `p`)
LEFT OUTER JOIN `products_description` as `pd` ON `p`.`products_id` = `pd`.`products_id`
LEFT OUTER JOIN `products_description_ncbi_sp` as `pdns` ON `p`.`products_id` = `pdns`.`products_id`
LEFT OUTER JOIN `products_extra` as `pe` ON `p`.`products_id` = `pe`.`products_id`
WHERE `p`.`products_id` = '30144'
AND `pd`.`language_id` = 1
LIMIT 1
select p.*, pd.*,
ifnull(pdns.ncbi_summary, 'N/A') as ncbi_summary_pdns,
ifnull(pdns.sp_comments, 'N/A') as sp_comments_pdns,
ifnull(pdns.ncbi_research_articles, 'N/A') as ncbi_research_articles_pdns,
ifnull(pe.products_description_extra, 'N/A') as products_description_extra
from products p
LEFT OUTER JOIN products_description pd on p.products_id = pd.products_id
LEFT OUTER JOIN products_description_ncbi_sp pdns on p.products_id = pdns.products_id
LEFT OUTER JOIN products_extra pe on p.products_id = pe.products_id
where p.products_id = '30144' and pd.language_id = 1
SELECT `options_values_price` as `price`, `products_options_values_name` as `package`
FROM `products_attributes`
JOIN `products_options_values` ON `products_options_values`.`products_options_values_id` = `products_attributes`.`options_values_id`
WHERE `products_attributes`.`products_id` = '30144'
⇄⧉testing_protocols => string (1290) "FCM (Flow Cytometry)||Flow cytometric analysis of Hela cells with MyD88 anti...
$value['testing_protocols']
FCM (Flow Cytometry)||Flow cytometric analysis of Hela cells with MyD88 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody||AAA30144_FCM6.jpg!!ICC (Immunocytochemistry)||ICC staining MyD88 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30144_ICC5.jpg!!ICC (Immunocytochemistry)||ICC staining MyD88 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30144_ICC4.jpg!!ICC (Immunocytochemistry)||ICC staining MyD88 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30144_ICC3.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MyD88 antibody. Counter stained with hematoxylin.||AAA30144_IHC2.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MyD88 antibody. Counter stained with hematoxylin.||AAA30144_IHC.jpg
⇄⧉products_description => string (911) "Interleukin-1 (IL-1)-induced activation of the NFkappaB pathway is mediated ...
$value['products_description']
Interleukin-1 (IL-1)-induced activation of the NFkappaB pathway is mediated through the IL-1 receptor and the subsequent phosphorylation of IL-1 receptor-associated kinase (IRAK). The myeloid differentiation protein MyD88 was originally characterized as a protein upregulated in myeloleukemic cells following IL-6-induced growth arrest and terminal differentiation. MyD88 is now known to function as an adaptor protein for the association of IRAK with the IL-1 receptor. MyD88 is functionally homologous to the adaptor protein tube in the Toll signaling pathway of Drosophilia, and both proteins are members of the Toll/IL-1R superfamily. MyD88 contains a characteristic N-terminal death domain that is essential for NFkappaB activation and an adjacent Toll/IL-1R homology domain (TIR domain). Collectively, these domains enable the protein-protein interactions of MyD88 with IRAK and the IL-1 receptor complex.
⇄products_references => string (3) "N/A"
$value['products_references']
⇄⧉products_related_diseases => string (212) "Inflammation||1034!!Necrosis||675!!Immune System Diseases||354!!Disease Mode...
$value['products_related_diseases']
Inflammation||1034!!Necrosis||675!!Immune System Diseases||354!!Disease Models, Animal||296!!Neoplasms||213!!Lung Diseases||179!!Cardiovascular Diseases||144!!Liver Diseases||108!!Pneumonia||92!!Drug Toxicity||81
⇄products_categories => string (16) "Total protein Ab"
⇄⧉testing_protocols => string (1290) "FCM (Flow Cytometry)||Flow cytometric analysis of Hela cells with MyD88 anti...
$value->a['testing_protocols']
FCM (Flow Cytometry)||Flow cytometric analysis of Hela cells with MyD88 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody||AAA30144_FCM6.jpg!!ICC (Immunocytochemistry)||ICC staining MyD88 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30144_ICC5.jpg!!ICC (Immunocytochemistry)||ICC staining MyD88 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30144_ICC4.jpg!!ICC (Immunocytochemistry)||ICC staining MyD88 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30144_ICC3.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MyD88 antibody. Counter stained with hematoxylin.||AAA30144_IHC2.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MyD88 antibody. Counter stained with hematoxylin.||AAA30144_IHC.jpg
⇄⧉products_description => string (911) "Interleukin-1 (IL-1)-induced activation of the NFkappaB pathway is mediated ...
$value->a['products_description']
Interleukin-1 (IL-1)-induced activation of the NFkappaB pathway is mediated through the IL-1 receptor and the subsequent phosphorylation of IL-1 receptor-associated kinase (IRAK). The myeloid differentiation protein MyD88 was originally characterized as a protein upregulated in myeloleukemic cells following IL-6-induced growth arrest and terminal differentiation. MyD88 is now known to function as an adaptor protein for the association of IRAK with the IL-1 receptor. MyD88 is functionally homologous to the adaptor protein tube in the Toll signaling pathway of Drosophilia, and both proteins are members of the Toll/IL-1R superfamily. MyD88 contains a characteristic N-terminal death domain that is essential for NFkappaB activation and an adjacent Toll/IL-1R homology domain (TIR domain). Collectively, these domains enable the protein-protein interactions of MyD88 with IRAK and the IL-1 receptor complex.
⇄products_references => string (3) "N/A"
$value->a['products_references']
⇄⧉products_related_diseases => string (212) "Inflammation||1034!!Necrosis||675!!Immune System Diseases||354!!Disease Mode...
$value->a['products_related_diseases']
Inflammation||1034!!Necrosis||675!!Immune System Diseases||354!!Disease Models, Animal||296!!Neoplasms||213!!Lung Diseases||179!!Cardiovascular Diseases||144!!Liver Diseases||108!!Pneumonia||92!!Drug Toxicity||81
⇄products_categories => string (16) "Total protein Ab"
⇄⧉testing_protocols => string (1290) "FCM (Flow Cytometry)||Flow cytometric analysis of Hela cells with MyD88 anti...
$value->d['testing_protocols']
FCM (Flow Cytometry)||Flow cytometric analysis of Hela cells with MyD88 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody||AAA30144_FCM6.jpg!!ICC (Immunocytochemistry)||ICC staining MyD88 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30144_ICC5.jpg!!ICC (Immunocytochemistry)||ICC staining MyD88 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30144_ICC4.jpg!!ICC (Immunocytochemistry)||ICC staining MyD88 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30144_ICC3.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MyD88 antibody. Counter stained with hematoxylin.||AAA30144_IHC2.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MyD88 antibody. Counter stained with hematoxylin.||AAA30144_IHC.jpg
⇄⧉products_description => string (911) "Interleukin-1 (IL-1)-induced activation of the NFkappaB pathway is mediated ...
$value->d['products_description']
Interleukin-1 (IL-1)-induced activation of the NFkappaB pathway is mediated through the IL-1 receptor and the subsequent phosphorylation of IL-1 receptor-associated kinase (IRAK). The myeloid differentiation protein MyD88 was originally characterized as a protein upregulated in myeloleukemic cells following IL-6-induced growth arrest and terminal differentiation. MyD88 is now known to function as an adaptor protein for the association of IRAK with the IL-1 receptor. MyD88 is functionally homologous to the adaptor protein tube in the Toll signaling pathway of Drosophilia, and both proteins are members of the Toll/IL-1R superfamily. MyD88 contains a characteristic N-terminal death domain that is essential for NFkappaB activation and an adjacent Toll/IL-1R homology domain (TIR domain). Collectively, these domains enable the protein-protein interactions of MyD88 with IRAK and the IL-1 receptor complex.
⇄products_references => string (3) "N/A"
$value->d['products_references']
⇄⧉products_related_diseases => string (212) "Inflammation||1034!!Necrosis||675!!Immune System Diseases||354!!Disease Mode...
$value->d['products_related_diseases']
Inflammation||1034!!Necrosis||675!!Immune System Diseases||354!!Disease Models, Animal||296!!Neoplasms||213!!Lung Diseases||179!!Cardiovascular Diseases||144!!Liver Diseases||108!!Pneumonia||92!!Drug Toxicity||81
⇄products_categories => string (16) "Total protein Ab"
⇄⧉specificity => string (376) "This assay has high sensitivity and excellent specificity for detection of N...
$value[0]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of NOX4. No significant cross-reactivity or interference between NOX4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NOX4 and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value[0]['_source']['purity']
⇄form => string (3) "N/A"
$value[0]['_source']['form']
⇄concentration => string (3) "N/A"
$value[0]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄⧉products_description => string (1390) "Principle of the Assay: NOX4 ELISA kit applies the competitive enzyme immuno...
$value[0]['_source']['products_description']
Principle of the Assay: NOX4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-NOX4 antibody and an NOX4-HRP conjugate. The assay sample and buffer are incubated together with NOX4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NOX4 concentration since NOX4 from samples and NOX4-HRP conjugate compete for the anti-NOX4 antibody binding site. Since the number of sites is limited, as more sites are occupied by NOX4 from the sample, fewer sites are left to bind NOX4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NOX4 concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This NOX4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine NOX4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄⧉specificity => string (169) "This assay has high sensitivity and excellent specificity for detection of N...
$value[1]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of NOX4. No significant cross-reactivity or interference between NOX4 and analogues was observed.
⇄purity => string (3) "N/A"
$value[1]['_source']['purity']
⇄form => string (3) "N/A"
$value[1]['_source']['form']
⇄concentration => string (3) "N/A"
$value[1]['_source']['concentration']
⇄⧉storage_stability => string (156) "Store entire kit at 2-8C for short-term. For longer-term, please store the m...
$value[1]['_source']['storage_stability']
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
NOX4/NADPH oxidase 4/Renal NAD (P)H-oxidase/Kidney oxidase-1/KOX-1/Kidney superoxide-producing NADPH oxidase/RENOX
⇄products_gene_name => string (4) "NOX4"
$value[1]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[1]['_source']['products_gene_name_syn']
⇄⧉products_description => string (825) "Principle of the Assay: This kit was based on sandwich enzyme-linked immune-...
$value[1]['_source']['products_description']
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.
⇄⧉search_terms => string (588) "aaa17633 human this assay has high sensitivity and excellent specificity for...
$value[1]['_source']['search_terms']
aaa17633 human this assay has high sensitivity and excellent specificity for detection of nox4 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa17633_sc elisa kit nadph oxidase 4 renal nad p h kidney 1 kox superoxide producing renox 59,915 da nox4_human 7739704 aaf68973.1 q9nph5 q5k3r4 q5k3r5 q5k3r6 q5k3r8 q7z7g3 q86v92 a8k715 b7z520 e7emd7 605261 samples serum plasma tissue homogenates other biological fluids type quantitative sandwich range 0.156 10ng ml 0.094ng intra precision cv oxidase4 kidney1
⇄⧉testing_protocols => string (1471) "ICC (Immunocytochemistry)||ICC staining NOX4 in A549 cells (green). The nucl...
$value[2]['_source']['testing_protocols']
ICC (Immunocytochemistry)||ICC staining NOX4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30056_ICC8.jpg!!ICC (Immunocytochemistry)||ICC staining NOX4 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30056_ICC7.jpg!!ICC (Immunocytochemistry)||ICC staining NOX4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30056_ICC6.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-NOX4 antibody. Counter stained with hematoxylin.||AAA30056_IHC5.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-NOX4 antibody. Counter stained with hematoxylin.||AAA30056_IHC4.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NOX4 antibody. Counter stained with hematoxylin.||AAA30056_IHC3.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti-NOX4 antibody. Counter stained with hematoxylin.||AAA30056_IHC2.jpg!!WB (Western Blot)||Western blot analysis of NOX4 on PC-12 cell lysates using anti-NOX4 antibody at 1/1, 000 dilution.||AAA30056_WB.jpg
⇄⧉products_description => string (1060) "The superoxide-generating NADPH oxidase includes a membrane-bound flavocytoc...
$value[2]['_source']['products_description']
The superoxide-generating NADPH oxidase includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with the flavocytochrome, cytochrome b558, to form the active enzyme complex. The p22 and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. Nox4 (also known as Renox) is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. Nox4 is also expressed in fetal tissues, placenta, glioblastoma and vascular cells. Like gp91-phox, the enzymatic activity of Nox4 produces superoxide anions. In vascular cells, the addition of Angiotensin II increases Nox4 expression, which suggests a role for Nox4 in vascular oxidative stress response. The gene encoding human Nox4 maps to chromosome 11q14.2-q21.
⇄⧉search_terms => string (958) "aaa30056 rabbit human mouse rat monoclonal sy0214 proa affinity purified 1*t...
$value[2]['_source']['search_terms']
aaa30056 rabbit human mouse rat monoclonal sy0214 proa affinity purified 1*tbs ph7.4 1 bsa 40 glycerol preservative 0.05 sodium azide western blot wb immunocytochemistry icc immunohistochemistry ihc immunoprecipitation ip 1:1000 1:50 1:200 1:100 analysis of nox4 on pc 12 cell lysates using anti antibody at 000 dilution aaa30056_wb immunohistochemical paraffin embedded stomach tissue counter stained with hematoxylin aaa30056_ihc2 kidney aaa30056_ihc3 aaa30056_ihc4 lung aaa30056_ihc5 staining in hela cells green the nuclear stain is dapi blue were fixed paraformaldehyde permeabilised 0.25 triton x100 pbs aaa30056_icc6 a431 aaa30056_icc7 a549 aaa30056_icc8 nadph oxidase 4 superoxide producing kox nox4_human renal nad p h renox isoform c 66,932 da 219842348 np_001137309.1 q9nph5 nm_001143837.1 q5k3r4 q5k3r5 q5k3r6 q5k3r8 q7z7g3 q86v92 a8k715 605261 total protein ab type recombinant immunogen conjugation unconjugated ph7.41 bsa40 pc12 at000 oxidase4
⇄⧉specificity => string (181) "This assay has high sensitivity and excellent specificity for detection of h...
$value[3]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of human NOX4. No significant cross-reactivity or interference between human NOX4 and analogues was observed.
⇄purity => string (3) "N/A"
$value[3]['_source']['purity']
⇄form => string (3) "N/A"
$value[3]['_source']['form']
⇄concentration => string (3) "N/A"
$value[3]['_source']['concentration']
⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
$value[3]['_source']['storage_stability']
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (326) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
$value[3]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%.Three samples of known concentration were tested in twenty assays to assess.
⇄⧉products_description => string (731) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
$value[3]['_source']['products_description']
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NOX4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NOX4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NOX4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NOX4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄⧉search_terms => string (621) "aaa18015 human this assay has high sensitivity and excellent specificity for...
$value[3]['_source']['search_terms']
aaa18015 human this assay has high sensitivity and excellent specificity for detection of nox4 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa18015_td elisa kit nadph oxidase 4 kox 1 renox kidney superoxide producing renal nad p h isoform b 66,932 da nox4_human 219842346 np_001137308.1 q9nph5 nm_001143836.2 q5k3r4 q5k3r5 q5k3r6 q5k3r8 q7z7g3 q86v92 a8k715 b7z520 e7emd7 605261 samples serum plasma tissue homogenates cell lysates type quantitative sandwich range 9.38 pg ml 600 < 2.34 intra precision within an cv oxidase4 kox1 ml600
⇄⧉storage_stability => string (490) "Storage: Avoid repeated freeze/thaw cycles.<br>Store at 2-8 degree C for one...
$value[4]['_source']['storage_stability']
Storage: Avoid repeated freeze/thaw cycles.<br>Store at 2-8 degree C for one month.<br>Aliquot and store at -80 degree C for 12 months.<br><br>Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
⇄app_tested => string (56) "Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)"
$value[4]['_source']['app_tested']
⇄⧉app_notes => string (76) "(May be suitable for use in other assays to be determined by the end user.) "
$value[4]['_source']['app_notes']
(May be suitable for use in other assays to be determined by the end user.)
Organism Species||Homo sapiens (Human)!!Source||Prokaryotic expression!!Residues||Met235~Asn488!!Tags||N-terminal His-Tag!!Subcellular Location||Membrane!!Traits||Freeze-dried powder!!Predicted isoelectric point||6.3!!Usage||Reconstitute in 20mM Tris, 150mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
⇄⧉search_terms => string (951) "aaa20230 e coli > 97 20mm tris 150mm nacl ph8.0 containing 1mm edta dtt 0.01...
$value[4]['_source']['search_terms']
aaa20230 e coli > 97 20mm tris 150mm nacl ph8.0 containing 1mm edta dtt 0.01 skl 5 trehalose and proclin 300 positive control immunogen sds page western blot wb may be suitable for use in other assays to determined by the end user
figure aaa20230_sds sequence aaa20230_seq recombinant protein nicotinamide adenine dinucleotide phosphate oxidase 1 nox1 nadph isoform 3 mox1 noh1 noh gp91 2 mitogenic homolog nadh subunit p65 mox pyridine nucleotide dependent superoxide generating predicted 30.8kda accurate 32kda as reducing conditions nox nox1_human 425854820 np_001258744.1 q9y5s8 nm_001271815.1 o95691 q2pp02 a8k836 300225 organism species homo sapiens human source prokaryotic expression residues met235~asn488 tags n terminal his tag subcellular location membrane traits freeze dried powder isoelectric point 6.3 usage reconstitute ph 8.0 a concentration of 0.1 1.0 mg ml do not vortex >97 skl5 proclin300 oxidase1 isoform3 gp912 point6.3 of0.1
⇄⧉specificity => string (376) "This assay has high sensitivity and excellent specificity for detection of N...
$value[5]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of NOX2. No significant cross-reactivity or interference between NOX2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NOX2 and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value[5]['_source']['purity']
⇄form => string (3) "N/A"
$value[5]['_source']['form']
⇄concentration => string (3) "N/A"
$value[5]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄⧉products_description => string (1389) "Principle of the Assay: NOX2 ELISA kit applies the competitive enzyme immuno...
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Principle of the Assay: NOX2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-NOX2 antibody and an NOX2-HRP conjugate. The assay sample and buffer are incubated together with NOX2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NOX2 concentration since NOX2 from samples and NOX2-HRP conjugate compete for the anti-NOX2 antibody binding site. Since the number of sites is limited, as more sites are occupied by NOX2 from the sample, fewer sites are left to bind NOX2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NOX2 concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This NOX2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human NOX2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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⇄⧉search_terms => string (570) "aaa27075 human this assay has high sensitivity and excellent specificity for...
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aaa27075 human this assay has high sensitivity and excellent specificity for detection of nox2 no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa27075_sc elisa kit nicotinamide adenine dinucleotide phosphate oxidase 2 samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 0.1 ng ml oxidase2 competitive0.1
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FCM (Flow Cytometry)||Flow cytometric analysis of Hela cells with NADPH oxidase 4 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.||AAA29878_FCM6.jpg!!ICC (Immunocytochemistry)||Immunocytochemical staining of Hela cells using anti-NADPH oxidase 4 rabbit polyclonal antibody.||AAA29878_ICC5.jpg!!ICC (Immunocytochemistry)||Immunocytochemical staining of 293T cells using anti-NADPH oxidase 4 rabbit polyclonal antibody.||AAA29878_ICC4.jpg!!ICC (Immunocytochemistry)||Immunocytochemical staining of HepG2 cells using anti-NADPH oxidase 4 rabbit polyclonal antibody.||AAA29878_ICC3.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin- embedded mouse kidney tissue using anti-NADPH oxidase 4 rabbit polyclonal antibody.||AAA29878_IHC2.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-NADPH oxidase 4 rabbit polyclonal antibody.||AAA29878_IHC.jpg
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The superoxide-generating NADPH oxidase includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with the flavocytochrome, cytochrome b558, to form the active enzyme complex. The p22 and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. Nox4 (also known as Renox) is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. Nox4 is also expressed in fetal tissues, placenta, glioblastoma and vascular cells. Like gp91-phox, the enzymatic activity of Nox4 produces superoxide anions. In vascular cells, the addition of Angiotensin II increases Nox4 expression, which suggests a role for Nox4 in vascular oxidative stress response. The gene encoding human Nox4 maps to chromosome 11q14.2-q21
⇄⧉search_terms => string (892) "aaa29878 rabbit human mouse rat polyclonal peptide affinity purified 1*tbs p...
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aaa29878 rabbit human mouse rat polyclonal peptide affinity purified 1*tbs ph7.4 1 bsa 40 glycerol preservative 0.05 sodium azide immunocytochemistry icc immunohistochemistry ihc flow cytometry fc facs 1:50 1:200 1:10 1:100 immunohistochemical analysis of paraffin embedded kidney tissue using anti nadph oxidase 4 antibody aaa29878_ihc aaa29878_ihc2 immunocytochemical staining hepg2 cells aaa29878_icc3 293t aaa29878_icc4 hela aaa29878_icc5 cytometric with at 50 dilution blue compared an unlabelled control without incubation primary red alexa fluor 488 conjugated goat igg was used as the secondary aaa29878_fc6 superoxide producing kox kox1 nox4 nox4_human renal nad p h renox isoform c 66,932 da 219842348 np_001137309.1 q9nph5 nm_001143837.1 q5k3r4 q5k3r5 q5k3r6 q5k3r8 q7z7g3 q86v92 a8k715 605261 total protein ab immunogen conjugation unconjugated ph7.41 bsa40 oxidase4 at50 fluor488
⇄⧉products_description => string (824) "Principle of the Assay: As mentioned above, this kit utilizes the Double Ant...
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Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Rat NOX2 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
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Adaptive Immune System Pathway||366160!!Antigen Processing-Cross Presentation Pathway||477122!!BDNF Signaling Pathway||712093!!Class I MHC Mediated Antigen Processing & Presentation Pathway||366161!!Cross-presentation Of Particulate Exogenous Antigens (phagosomes) Pathway||477123!!Disease Pathway||530764!!Immune System Pathway||106386!!Latent Infection Of Homo Sapiens With Mycobacterium Tuberculosis Pathway||645267!!Leishmaniasis Pathway||144181!!Leishmaniasis Pathway||144028
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This assay has high sensitivity and excellent specificity for detection of NAD. No significant cross-reactivity or interference between NAD and analogues was observed.
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.<br>To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay Type||Quantitative Competitive!!Samples||Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids!!Detection Range||123.5-10,000ng/mL!!Sensitivity||< 49.3ng/mL
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NAD were tested 20 times on one plate, respectively. Intra-Assay: CV<10%!!Inter-assay Precision||Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NAD were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
⇄⧉products_description => string (978) "Principle of the Assay: This assay employs the competitive inhibition enzyme...
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Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to NAD has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled NAD and unlabeled NAD (Standards or samples) with the pre-coated antibody specific to NAD. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of NAD in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of NAD in the sample.<br><br>Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of NAD in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
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aaa22856 general this assay has high sensitivity and excellent specificity for detection of nad no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa22856_sc elisa kit nicotinamide adenine dinucleotide partial mitochondrion 32,576 da nadh ubiquinone oxidoreductase chain 2 nadh2 555289422 cdh35302.1 samples serum plasma tissue homogenates cell lysates culture supernates other biological fluids type quantitative competitive range 123.5 10,000ng ml < 49.3ng intra precision within an 3 with low middle level were tested 20 times on one plate respectively cv<10 inter assays different plates 8 replicates in each cv = sd meanx100 cv<12 chain2 an3 tested20 plates8
⇄⧉products_description => string (826) "Principle of the Assay: As mentioned above, this kit utilizes the Double Ant...
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Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Mouse NOX2 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
⇄⧉search_terms => string (382) "aaa13182 mouse no cross reaction with other factors typical testing data sta...
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aaa13182 mouse no cross reaction with other factors typical testing data standard curve for reference only aaa13182_sc elisa kit nicotinamide adenine dinucleotide phosphate oxidase 2 nox2 samples serum plasma or cell culture supernatant assay type quantitative sandwich detection range 10 ng ml 0.156 sensitivity up to 0.05 intra precision <= 8 inter 12 oxidase2 range10 <=8 inter12
⇄⧉specificity => string (171) "This assay has high sensitivity and excellent specificity for detection of N...
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This assay has high sensitivity and excellent specificity for detection of NAADP. No significant cross-reactivity or interference between NAADP and analogues was observed.
⇄purity => string (3) "N/A"
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⇄⧉storage_stability => string (475) "The stability of kit is determined by the loss rate of activity. The loss ra...
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. <br>To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay Type||Quantitative Competitive!!Samples||Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids!!Detection Range||123.5-10,000ng/mL!!Sensitivity||< 48.7ng/mL
⇄⧉etc_term2 => string (416) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): 3 ...
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NAADP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%!!Inter-assay Precision||Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NAADP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
⇄⧉products_description => string (986) "Principle of the Assay: This assay employs the competitive inhibition enzyme...
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Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to NAADP has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled NAADP analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of NAADP in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of NAADP in the sample.<br><br>Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of NAADP in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
⇄⧉search_terms => string (651) "aaa20048 general this assay has high sensitivity and excellent specificity f...
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aaa20048 general this assay has high sensitivity and excellent specificity for detection of naadp no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa20048_td elisa kit nicotinic acid adenine dinucleotide phosphate samples serum plasma tissue homogenates cell lysates culture supernates other biological fluids type quantitative competitive range 123.5 10,000ng ml <48.7ng intra precision within an 3 with low middle level were tested 20 times on one plate respectively cv<10 inter assays different plates 8 replicates in each cv = sd meanx100 cv<12 an3 tested20 plates8
⇄⧉testing_protocols => string (1121) "FCM (Flow Cytometry)||Flow cytometric analysis of HepG2 cells with SIRT3 ant...
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FCM (Flow Cytometry)||Flow cytometric analysis of HepG2 cells with SIRT3 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.||AAA29877_FCM6.jpg!!ICC (Immunocytochemistry)||Immunocytochemical staining of HepG2 cells using anti-SIRT3 rabbit polyclonal antibody.||AAA29877_ICC5.jpg!!ICC (Immunocytochemistry)||Immunocytochemical staining of MCF-7 cells using anti-SIRT3 rabbit polyclonal antibody.||AAA29877_ICC4.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin- embedded mouse kidney tissue using anti-SIRT3 rabbit polyclonal antibody.||AAA29877_IHC3.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-SIRT3 rabbit polyclonal antibody.||AAA29877_IHC2.jpg!!WB (Western Blot)||Western blot analysis on different lysates using anti-SIRT3 rabbit polyclonal antibody. Positive control: Lane 1: A172 Lane 2: Mouse liver Lane 3: NIH/3T3 Lane 4: Mouse kidney Lane 5: F9||AAA29877_WB.jpg
hSIRT 3 antibody; hSIRT3 antibody; Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody; NAD dependent deacetylase sirtuin 3 mitochondrial antibody; NAD-dependent protein deacetylase sirtuin-3; mitochondrial antibody; Regulatory protein SIR2 homolog 3 antibody; Silent mating type information regulation 2 S.cerevisiae homolog 3 antibody; Sir 2 like 3 antibody; SIR 2 like protein 3 antibody; SIR 3 antibody; SIR2 L3 antibody; Sir2 like 3 antibody; SIR2 like protein 3 antibody; SIR2-like protein 3 antibody; SIR2L3 antibody; SIR3_HUMAN antibody; SIRT 3 antibody; SIRT3 antibody; Sirtuin 3 antibody; Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae) antibody; Sirtuin type 3 antibody; Sirtuin3 antibody
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⇄⧉products_description => string (990) "The Silent Information Regulator (SIR2) family of genes are highly conserved...
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The Silent Information Regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. In S. cerevisiae, Sir2p deacetylates histones in an NAD-dependent manner, which regulates silencing at the telomeric, rDNA and silent mating-type loci. Sir2p is the founding member of a large family, designated sirtuins, which contain a conserved catalytic domain. The human homologues, which include SIRT1-7, are divided into four main branches: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. SIRT3 is a NAD-dependent deacetylase that contains one deacetylase sirtuin-type domain. The SIRT3 protein is widely expressed and localizes to the mitochondira where it is processed by mitochondrial processing peptidase (MPP) to yield a final product. This processing is most-likely necessary for its enzymatic activity.
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⇄⧉products_related_diseases => string (218) "Cardiovascular Diseases||21!!Nervous System Diseases||15!!Fatty Liver||15!!L...
NAD-dependent protein deacetylase sirtuin-3, mitochondrial; sir2-like 3; sirtuin type 3; SIR2-like protein 3; regulatory protein SIR2 homolog 3; NAD-dependent deacetylase sirtuin-3, mitochondrial; silent mating type information regulation 2, S.cerevisiae,
⇄⧉ncbi_pathways => string (153) "Energy Metabolism Pathway||198907!!Signaling Events Mediated By HDAC Class I...
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Energy Metabolism Pathway||198907!!Signaling Events Mediated By HDAC Class I Pathway||137997!!Signaling Events Mediated By HDAC Class III Pathway||137972
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Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human NADPH Oxidase monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
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aaa22670 human typical testing data standard curve for reference only aaa22670_sc elisa kit nadph oxidase isoform c nox cg15924 cg34399 cg3896 dmelcg34399 dnox 1 nox1 gene product from transcript rd 161077140 np_001097336.1 nm_001103866.2 samples serum plasma or cell culture supernatant assay type quantitative sandwich detection range 20 ng ml 0.312 sensitivity up to 0.06 intra precision <= 8 inter 12 dnox1 range20 <=8 inter12
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Samples||Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate!!Assay Type||Quantitative Competitive or Sandwich!!Sensitivity||0.1 ng/mL.
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Intended Uses: This CYFRA 21.1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CYFRA 21.1. This ELISA kit for research use only!<br><br>Principle of the Assay: CYFRA 21.1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CYFRA 21.1. Standards or samples are then added to the microtiter plate wells and CYFRA 21.1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CYFRA 21.1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CYFRA 21.1 are added to each well to "sandwich" the CYFRA 21.1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CYFRA 21.1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYFRA 21.1 concentration in each sample is interpolated from this standard curve.
⇄sp_protein_name => string (45) "Cytochrome c oxidase subunit 4, mitochondrial"
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⇄sp_protein_name_syn => string (35) "Cytochrome c oxidase polypeptide IV"
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⇄sp_gene_name_syn => string (8) "NCU05689"
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⇄sp_entry_name => string (10) "COX4_NEUCR"
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aaa16379 mouse typical testing data standard curve for reference only aaa16379_sc elisa kit cytochrome oxidase 4 cox c subunit mitochondrial ncu05689 polypeptide iv precursor 20,613 da cox4_neucr 62297096 p06809.2 p06809 q7sbp9 samples serum plasma cell culture supernatants body fluid and tissue homogenate assay type quantitative competitive or sandwich sensitivity 0.1 ng ml oxidase4 sensitivity0.1
⇄⧉products_description => string (1134) "Principle of the Assay: Human Cytochrome b-245 Heavy Chain (CYBB) ELISA Kit ...
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Principle of the Assay: Human Cytochrome b-245 Heavy Chain (CYBB) ELISA Kit employs a two-site sandwich ELISA to quantitate CYBB in samples. An antibody specific for CYBB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYBB present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human Cytochrome b-245 heavy chain (CYBB) ELISA Kit CYBB detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of CYBB bound in the initial step. The color development is stopped and the intensity of the color is measured.<br><br>Background/Introduction: Cytochrome b-245 heavy chain (CYBB) encodes the heavy chain component of a heterodimeric transmembrane ion transporter composed of both a heavy and a light chain. This transporter mediates the transfer of electrons from nicotinamide adenine dinucleotide phosphate (NADPH) to oxygen to generate superoxide. This reaction is important in the innate immune response to pathogens.
AGE-RAGE Signaling Pathway In Diabetic Complications||1319988!!AGE-RAGE Signaling Pathway In Diabetic Complications||1319775!!Adaptive Immune System Pathway||1269171!!Antigen Processing-Cross Presentation Pathway||1269195!!Cellular Responses To Stress Pathway||1270414!!Class I MHC Mediated Antigen Processing & Presentation Pathway||1269192!!Cross-presentation Of Particulate Exogenous Antigens (phagosomes) Pathway||1269196!!Detoxification Of Reactive Oxygen Species Pathway||1270420!!Ferroptosis Pathway||1510435!!Ferroptosis Pathway||1510200
⇄sp_protein_name => string (28) "Cytochrome b-245 heavy chain"
⇄⧉specificity => string (181) "This assay has high sensitivity and excellent specificity for detection of m...
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This assay has high sensitivity and excellent specificity for detection of mouse NQO1. No significant cross-reactivity or interference between mouse NQO1 and analogues was observed.
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⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (328) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. !!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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⇄products_name => string (34) "NAD (P) H dehydrogenase, quinone 1"
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⇄products_name_oem => string (57) "Mouse NAD (P) H dehydrogenase [quinone] 1, NQO1 ELISA Kit"
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Mouse NAD (P) H dehydrogenase [quinone] 1 (NQO1) ELISA kit; DHQU; DIA4; DTD; NMOR1; NMORI; QR1; DT-diaphorase; NAD (P) H:Quinone acceptor oxidoreductase type 1; NAD (P) H:menadione oxidoreductase 1; NAD (P) H:quinone oxireductase; azoreductase; diaphorase (NADH/NADPH) ; NAD (P) H dehydrogenase; quinone 1
⇄products_gene_name => string (4) "NQO1"
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⇄⧉products_description => string (731) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NQO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NQO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NQO1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NQO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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aaa18122 mouse this assay has high sensitivity and excellent specificity for detection of nqo1 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa18122_td elisa kit nad p h dehydrogenase quinone 1 dhqu dia4 dtd nmor1 nmori qr1 dt diaphorase h:quinone acceptor oxidoreductase type h:menadione oxireductase azoreductase nadh nadph ox1 nmo1 ox nmo av001255 menadione reductase phylloquinone 4 dioxin inducible 30,960 da nqo1_mouse 94400773 np_032732.3 q64669 nm_008706.5 samples serum plasma tissue homogenates cell lysates quantitative sandwich range 31.25 pg ml 2000 < 7.81 intra precision within an cv <8 three known concentration were tested twenty times on one plate to assess inter assays <10 in quinone1 phylloquinone4
⇄⧉specificity => string (181) "This assay has high sensitivity and excellent specificity for detection of h...
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This assay has high sensitivity and excellent specificity for detection of human NQO1. No significant cross-reactivity or interference between human NQO1 and analogues was observed.
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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⇄products_name => string (34) "NAD (P) H dehydrogenase, quinone 1"
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⇄products_name_oem => string (56) "Human NAD (P) H Dehydrogenase, Quinone 1, NQO1 ELISA Kit"
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⇄⧉products_name_syn => string (304) "Human NAD (P) H Dehydrogenase; Quinone 1 (NQO1) ELISA Kit; DHQU; DIA4; DTD; ...
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Human NAD (P) H Dehydrogenase; Quinone 1 (NQO1) ELISA Kit; DHQU; DIA4; DTD; NMOR1; NMORI; QR1; DT-diaphorase; NAD (P) H:Quinone acceptor oxidoreductase type 1; NAD (P) H:menadione oxidoreductase 1; NAD (P) H:quinone oxireductase; azoreductase; diaphorase (NADH/NADPH) ; NAD (P) H dehydrogenase; quinone 1
⇄products_gene_name => string (4) "NQO1"
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⇄⧉products_description => string (731) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NQO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NQO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NQO1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NQO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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⇄⧉products_related_diseases => string (223) "Inflammation||128!!Liver Diseases||118!!Lung Neoplasms||100!!Nervous System ...
AhR Pathway||755436!!Dopamine Metabolism Pathway||920995!!Estrogen Metabolism Pathway||198898!!Keap1-Nrf2 Pathway||198777!!Metabolism Pathway||477135!!Metabolism Of Amino Acids And Derivatives Pathway||106169!!Oxidative Stress Pathway||198916!!Regulation Of Ornithine Decarboxylase (ODC) Pathway||106176!!Ubiquinone And Other Terpenoid-quinone Biosynthesis Pathway||82939!!Ubiquinone And Other Terpenoid-quinone Biosynthesis Pathway||300
⇄⧉search_terms => string (731) "aaa15710 human this assay has high sensitivity and excellent specificity for...
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aaa15710 human this assay has high sensitivity and excellent specificity for detection of nqo1 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa15710_td elisa kit nad p h dehydrogenase quinone 1 dhqu dia4 dtd nmor1 nmori qr1 dt diaphorase h:quinone acceptor oxidoreductase type h:menadione oxireductase azoreductase nadh nadph 4 dioxin inducible menadione reductase phylloquinone cytochrome b 5 30,868 da nqo1_human 4505415 np_000894.1 p15559 nm_000903.2 q86uk1 b2r5y9 b4dnm7 b7zad1 125860 samples serum plasma tissue homogenates cell lysates quantitative sandwich range 39 pg ml 2500 < 9.75 intra precision within an cv quinone1 nadph4 b5 range39
⇄⧉products_description => string (833) "Principle of the Assay: As mentioned above, this kit utilizes the Double Ant...
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Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Rat NADPH Oxidase monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
⇄⧉search_terms => string (408) "aaa22526 typical testing data standard curve for reference only aaa22526_sc ...
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aaa22526 typical testing data standard curve for reference only aaa22526_sc elisa kit nadph oxidase rat nox cg15924 cg34399 cg3896 dmelcg34399 dnox gene product from transcript rc pc 161077140 np_001097336.1 nm_001103866.1 samples serum plasma or cell culture supernatant assay type quantitative sandwich detection range 20 ng ml 0.312 sensitivity up to 0.06 intra precision <= 8 inter 12 range20 <=8 inter12
⇄⧉specificity => string (171) "This assay has high sensitivity and excellent specificity for detection of N...
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This assay has high sensitivity and excellent specificity for detection of NAMPT. No significant cross-reactivity or interference between NAMPT and analogues was observed.
⇄purity => string (3) "N/A"
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⇄form => string (3) "N/A"
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⇄concentration => string (3) "N/A"
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⇄⧉storage_stability => string (156) "Store entire kit at 2-8C for short-term. For longer-term, please store the m...
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Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
⇄⧉products_description => string (825) "Principle of the Assay: This kit was based on sandwich enzyme-linked immune-...
$value[18]['_source']['products_description']
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.
⇄⧉specificity => string (376) "This assay has high sensitivity and excellent specificity for detection of C...
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This assay has high sensitivity and excellent specificity for detection of CYBB. No significant cross-reactivity or interference between CYBB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CYBB and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
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⇄form => string (3) "N/A"
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⇄concentration => string (3) "N/A"
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⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄⧉products_description => string (1381) "Intended Uses: This CYBB ELISA kit is a 1.5 hour solid-phase ELISA designed ...
$value[19]['_source']['products_description']
Intended Uses: This CYBB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CYBB. This ELISA kit for research use only, not for therapeutic or test applications!<br><br>Principle of the Assay: CYBB ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CYBB antibody and an CYBB-HRP conjugate. The assay sample and buffer are incubated together with CYBB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CYBB concentration since CYBB from samples and CYBB-HRP conjugate compete for the anti-CYBB antibody binding site. Since the number of sites is limited, as more sites are occupied by CYBB from the sample, fewer sites are left to bind CYBB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYBB concentration in each sample is interpolated from this standard curve.
⇄⧉ncbi_pathways => string (527) "Adaptive Immune System Pathway||366160!!Antigen Processing-Cross Presentatio...
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Adaptive Immune System Pathway||366160!!Antigen Processing-Cross Presentation Pathway||477122!!Class I MHC Mediated Antigen Processing & Presentation Pathway||366161!!Cross-presentation Of Particulate Exogenous Antigens (phagosomes) Pathway||477123!!Disease Pathway||530764!!HIF-1 Signaling Pathway||695200!!Immune System Pathway||106386!!Latent Infection Of Homo Sapiens With Mycobacterium Tuberculosis Pathway||645267!!Leukocyte Transendothelial Migration Pathway||83083!!Leukocyte Transendothelial Migration Pathway||494
⇄sp_protein_name => string (28) "Cytochrome b-245 heavy chain"
⇄⧉search_terms => string (847) "aaa27239 rat this assay has high sensitivity and excellent specificity for d...
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aaa27239 rat this assay has high sensitivity and excellent specificity for detection of cybb no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa27239_sc elisa kit cytochrome b 245 heavy chain beta polypeptide cgd nox2 imd34 amcbx2 gp91 1 gp91phox p91 phox cgd91 nadph oxidase 2 p22 phagocyte b558 subunit neutrophil 91 kda heme binding membrane glycoprotein superoxide generating 65,336 da 558 cy24b_human 6996021 np_000388.2 p04839 nm_000397.3 q2pp16 a8k138 306400 samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 0.1 ng ml b245 gp911 oxidase2 neutrophil91 da558 competitive0.1
