Rabbit anti-Human SOX2 Polyclonal Antibody | anti-SOX2 antibody

Rabbit Polyclonal (IgG) to Human SOX2

Cat. Num. AAA241751

• Gene Names: SOX2; ANOP3; MCOPS3
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: SOX2; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human SOX2; Anti-SOX2 Antibody (N-Terminus) IHC-plus; MCOPS3; Transcription factor SOX2; ANOP3; Transcription factor SOX-2; SRY-related HMG-box gene 2; Human SOX2; anti-SOX2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Peptide-KLH (N-terminal)
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Phosphate-buffered solution, pH 7.2, 0.09% sodium azide, 50% glycerol.
• Concentration: 0.25 mg/ml (varies by lot)
• Sequence Length: 317

• Applicable Applications for anti-SOX2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (1:50), WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human SOX2.
• Immunogen Type: Synthetic peptide
• Immunogen: SOX2 antibody was raised against synthetic peptide from human SOX2.
• Antigen Modification: N-Terminus
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-SOX2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)

Western Blot (WB)

(NTERA-2 whole cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal antibody raised against the N-terminal region of SOX2. Proteins were visualized using HRP Donkey anti-rabbit IgG and a chemiluminescence detection system.)

Related Product Information for anti-SOX2 antibody

SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. SOX2 is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation.

Product Categories/Family for anti-SOX2 antibody

Family: Transcription factor

NCBI and Uniprot Product Information

• NCBI GI #: 28195386
• NCBI GeneID: 6657
• NCBI Accession #: NP_003097.1
• NCBI GenBank Nucleotide #: NM_003106.3
• UniProt Accession #: P48431; Q14537
• Molecular Weight: 34,310 Da
• NCBI Official Full Name: transcription factor SOX-2
• NCBI Official Synonym Full Names: SRY (sex determining region Y)-box 2
• NCBI Official Symbol: SOX2
• NCBI Official Synonym Symbols: ANOP3; MCOPS3
• NCBI Protein Information: transcription factor SOX-2; transcription factor SOX2; SRY-related HMG-box gene 2
• UniProt Protein Name: Transcription factor SOX-2
• UniProt Gene Name: SOX2
• UniProt Entry Name: SOX2_HUMAN

NCBI Description

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008]

Uniprot Description

SOX2: Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Interacts with ZSCAN10. Interacts with SOX3 and FGFR1.

Protein type: Cell development/differentiation; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 3q26.3-q27

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; miRNA binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; tongue development; establishment and/or maintenance of chromatin architecture; somatic stem cell maintenance; endodermal cell fate specification; positive regulation of transcription, DNA-dependent; diencephalon morphogenesis; negative regulation of transcription from RNA polymerase II promoter; regulation of caspase activity; olfactory placode formation; forebrain neuron differentiation; detection of mechanical stimulus involved in equilibrioception; positive regulation of MAPKKK cascade; regulation of transcription, DNA-dependent; tissue regeneration; forebrain development; male genitalia development; positive regulation of cell-cell adhesion; response to wounding; adenohypophysis development; positive regulation of epithelial cell differentiation; negative regulation of osteoblast differentiation; cell cycle arrest; inner ear development; positive regulation of Notch signaling pathway; negative regulation of epithelial cell proliferation; positive regulation of neuroblast proliferation; inner ear morphogenesis; response to retinoic acid; neuron fate commitment; detection of mechanical stimulus involved in sensory perception of sound; osteoblast differentiation; negative regulation of neuron differentiation; eye development; regulation of gene expression; pituitary gland development; glial cell fate commitment; positive regulation of transcription from RNA polymerase II promoter; cerebral cortex development; pigment biosynthetic process; positive regulation of neuron differentiation; alveolus development; retina morphogenesis in camera-type eye

Disease: Microphthalmia, Syndromic 3; Tracheoesophageal Fistula With Or Without Esophageal Atresia