Western Blot (WB)
((0.1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat SOX10 Polyclonal Antibody | anti-SOX10 antibody
Goat anti-SOX10 Antibody
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig
WB: Approx 50kDa band observed in Human Brain (Cerebellum) and approx. 52kDa in Mouse Brain lysates (calculated MW of 49.9kDa according to Human NP_008872.1 and Mouse NP_035567.1). Recommended concentration: 0.03-0.1µg/ml. Primary incubation 1 hour at room temperature.
IF: Strong expression of the protein seen in the nucleus of KNRK cells. Recommended concentration: 10µg/ml.
Western Blot (WB)
((0.1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(MBS423335 (0.1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.)
Western Blot (WB)
(MBS423335 (0.1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.)
Immunofluorescence (IF)
(MBS423335 Immunofluorescence analysis of paraformaldehyde fixed KNRK cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
Immunofluorescence (IF)
(MBS423335 Immunofluorescence analysis of paraformaldehyde fixed KNRK cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. [provided by RefSeq, Jul 2008]
Uniprot Description
SOX10: Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia. Defects in SOX10 are the cause of Waardenburg syndrome type 2E (WS2E). WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1. Defects in SOX10 are a cause of Waardenburg syndrome type 4C (WS4C); also known as Waardenburg-Shah syndrome. WS4C is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease). Defects in SOX10 are the cause of peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease (PCWH); also called neurologic variant of Waardenburg-Shah syndrome. PCWH is a rare, complex and more severe neurocristopathy that includes features of 4 distinct syndromes: peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease.
Protein type: Transcription factor; DNA-binding; Cell development/differentiation
Chromosomal Location of Human Ortholog: 22q13.1
Cellular Component: extrinsic to mitochondrial outer membrane; nucleoplasm; nucleus
Molecular Function: chromatin binding; identical protein binding; protein binding; RNA polymerase II transcription factor activity, enhancer binding; transcription coactivator activity; transcription factor binding
Biological Process: anatomical structure morphogenesis; cell maturation; developmental growth; digestive tract morphogenesis; enteric nervous system development; in utero embryonic development; lacrimal gland development; melanocyte differentiation; negative regulation of apoptosis; negative regulation of transcription, DNA-dependent; neural crest cell migration; oligodendrocyte differentiation; peripheral nervous system development; positive regulation of gliogenesis; positive regulation of neuroblast proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter
Disease: Peripheral Demyelinating Neuropathy, Central Dysmyelination, Waardenburg Syndrome, And Hirschsprung Disease; Waardenburg Syndrome, Type 2e; Waardenburg Syndrome, Type 4c
Research Articles on SOX10
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Product Notes
The SOX10 sox10 (Catalog #AAA423335) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-SOX10 Antibody reacts with Tested: Human, Mouse, Rat Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's SOX10 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). EIA (peptide): antibody detection limit dilution 1:128000. WB: Approx 50kDa band observed in Human Brain (Cerebellum) and approx. 52kDa in Mouse Brain lysates (calculated MW of 49.9kDa according to Human NP_008872.1 and Mouse NP_035567.1). Recommended concentration: 0.03-0.1µg/ml. Primary incubation 1 hour at room temperature. IF: Strong expression of the protein seen in the nucleus of KNRK cells. Recommended concentration: 10µg/ml. Researchers should empirically determine the suitability of the SOX10 sox10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DAKAQVKTET AGPQ. It is sometimes possible for the material contained within the vial of "SOX10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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