Rabbit anti-Human Solute Carrier Family 10 Polyclonal Antibody | anti-SLC11A1 antibody

Solute Carrier Family 10, ID (SLC11A1, Natural Resistance-associated Macrophage Protein 1, NRAMP 1, NRAMP1, NRAMP, LSH) (Azide free) (HRP)

Cat. Num. AAA6500253

• Gene Names: SLC11A1; LSH; NRAMP; NRAMP1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Solute Carrier Family 10; Polyclonal Antibody; ID (SLC11A1; Natural Resistance-associated Macrophage Protein 1; NRAMP 1; NRAMP1; NRAMP; LSH) (Azide free) (HRP); anti-SLC11A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SLC11A1.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SLC11A1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human SLC11A1 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SLC11A1 Antibody (Center) in CEM cell line lysates (35ug/lane). SLC11A1 (arrow) was detected using the purified Pab.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of SLC11A1 Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-SLC11A1 antibody

Antibodies; Ion Channel

References

1. Jin, J., et.al., Zhongguo Dang Dai Er Ke Za Zhi 11(4), 283-287 (2009). 2. Liu, J., et.al., Am. J. Hum. Genet. 56(4), 845-853 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 109255241
• NCBI GeneID: 6556
• NCBI Accession #: NP_000569.3
• NCBI GenBank Nucleotide #: NM_000578.4
• UniProt Accession #: P49279; C0H5Y3
• Molecular Weight: 47,292 Da
• NCBI Official Full Name: natural resistance-associated macrophage protein 1
• NCBI Official Synonym Full Names: solute carrier family 11 member 1
• NCBI Official Symbol: SLC11A1
• NCBI Official Synonym Symbols: LSH; NRAMP; NRAMP1
• NCBI Protein Information: natural resistance-associated macrophage protein 1
• UniProt Protein Name: Natural resistance-associated macrophage protein 1
• Protein Family: Solute carrier family
• UniProt Gene Name: SLC11A1
• UniProt Synonym Gene Names: LSH; NRAMP; NRAMP1; NRAMP 1

NCBI Description

This gene is a member of the solute carrier family 11 (proton-coupled divalent metal ion transporters) family and encodes a multi-pass membrane protein. The protein functions as a divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Mutations in this gene have been associated with susceptibility to infectious diseases such as tuberculosis and leprosy, and inflammatory diseases such as rheumatoid arthritis and Crohn disease. Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Macrophage-specific membrane transport function. Controls natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe2+ and Mn2+, cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes.