Rabbit anti-Human Sodium-dependent Vitamin C Transporter 1 Polyclonal Antibody | anti-SLC23A1 antibody

Sodium-dependent Vitamin C Transporter 1 (SVCT1)

Cat. Num. AAA614471

• Gene Names: SLC23A1; SVCT1; YSPL3; SLC23A2
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Sodium-dependent Vitamin C Transporter 1; Polyclonal Antibody; Sodium-dependent Vitamin C Transporter 1 (SVCT1); Anti -Sodium-dependent Vitamin C Transporter 1 (SVCT1); anti-SLC23A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SVCT1. No significant homology exist with SVCT2 or other related transporters. Species Sequence Homology (40-45%): Mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute in 100ul sterile PBS.

• Applicable Applications for anti-SLC23A1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Dilution: ELISA: 0.5-1ug/ml; S5327-40A Control peptide can be used to coat ELISA plates at 1ug/ml.; Western Blot: 1-10ug/ml using ECL technique.
• Immunogen: Synthetic peptide, 15aa, corresponding to ~N-terminal, cytoplasmic domain of human SVCT.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-SLC23A1 antibody

A sodium-dependent transport system is responsible for transfer and distribution of vitamins to different parts of the body, the transfer includes vitamins like pantothenate, biotin, and ascorbic acid etc, These transporters belong to Solute Carrier family (SLC). Since vitamins are required for essential metabolic processes in all mammalian cells, such cells have developed intrinsic mechanisms to active accumulation of essential vitamins. Thus transporters help these cells to fulfill their requirement, they include Sodium-dependent Multi-Vitamin Transporter (SMVT), Sodium-dependent Vitamin-C Transporter (SVCT) 1 & 2, Creatine Transporter (CRT1/ CT1). The other vitamin transporters from SLC family include Thiamine Transporter Protein 1 (THTR1), Folate Transporter or Reduced Folate Carrier 1 (RFC1), Thyroid Iodide Transporter (TIT) and Taurine Transporter (TAU) etc. Sodium-dependent Vitamin C Transporter (SVCT), Vitamin C is now known to mediate a variety of enzymatic reactions, including collagen synthesis, the basis for the defect in scurvy, the vitamin also protects tissues from oxidative damage by scavenging free radical. The vitamin C absorption and distribution requires SVCT1 and SVCT2. SVCT1 605aa protein in rat, 604 in human (chrm 5, gene SLC23A1) contain up to 12 transmembrane domains, two possible sites for N-glycosylation and multiple phosphorylation sites. It is 65% identical to SVCT2 and largely expressed in epithelial surfaces involved in bulk transport such as intestine, liver and kidney.

Product Categories/Family for anti-SLC23A1 antibody

Antibodies; Abs to Transport Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 44680145
• NCBI GeneID: 9963
• NCBI Accession #: NP_005838.3
• NCBI GenBank Nucleotide #: NM_005847.4
• UniProt Accession #: Q9UHI7; O95191; Q8WWB6; Q9UGH4; Q9UI39
• Molecular Weight: 64,831 Da
• NCBI Official Full Name: solute carrier family 23 member 1 isoform a
• NCBI Official Synonym Full Names: solute carrier family 23 (ascorbic acid transporter), member 1
• NCBI Official Symbol: SLC23A1
• NCBI Official Synonym Symbols: SVCT1; YSPL3; SLC23A2
• NCBI Protein Information: solute carrier family 23 member 1; hSVCT1; yolk sac permease-like molecule 3; Na(+)/L-ascorbic acid transporter 1; sodium-dependent vitamin C transporter 1; sodium-dependent vitamin C transporter-1; solute carrier family 23 (nucleobase transporters), member 1; solute carrier family 23 (nucleobase transporters), member 2
• UniProt Protein Name: Solute carrier family 23 member 1
• Protein Family: Solute carrier family
• UniProt Gene Name: SLC23A1
• UniProt Synonym Gene Names: SVCT1; YSPL3; hSVCT1
• UniProt Entry Name: S23A1_HUMAN

NCBI Description

The absorption of vitamin C into the body and its distribution to organs requires two sodium-dependent vitamin C transporters. This gene encodes one of the two transporters. The encoded protein is active in bulk vitamin C transport involving epithelial surfaces. Previously, this gene had an official symbol of SLC23A2. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]

Uniprot Description

SLC23A1: Sodium/ascorbate cotransporter. Mediates electrogenic uptake of vitamin C, with a stoichiometry of 2 Na(+) for each ascorbate. Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q31.2

Cellular Component: membrane; integral to plasma membrane; apical plasma membrane; cytoplasm; basal plasma membrane; plasma membrane; intracellular organelle; brush border

Molecular Function: L-ascorbate:sodium symporter activity; protein binding; dehydroascorbic acid transporter activity; sodium ion transmembrane transporter activity; L-ascorbic acid transporter activity; nucleobase transmembrane transporter activity

Biological Process: vitamin metabolic process; response to toxin; sodium ion transport; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; L-ascorbic acid metabolic process; nucleobase transport; brain development; water-soluble vitamin metabolic process; lung development; L-ascorbic acid transport