Rabbit SOD1 (UbetaB) Polyclonal Antibody | anti-SOD1 antibody

SOD1 (UbetaB) Antibody: ATTO 390

Cat. Num. AAA805364

• Gene Names: SOD1; ALS; SOD; ALS1; IPOA; hSod1; HEL-S-44; homodimer
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Synonyms: SOD1 (UbetaB); Polyclonal Antibody; SOD1 (UbetaB) Antibody: ATTO 390; Superoxide dismutase 1; SOD; SOD1 unfolded beta barrel region; anti-SOD1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Form/Format: Affinity Purified
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 154

• Applicable Applications for anti-SOD1 antibody: Western Blot (WB)
• Application Notes: 1:1000 (WB)
• Conjugate: ATTO 390
• Storage Buffer: PBS, 50% glycerol and 0.09% sodium azide.
• Preparation and Storage: -20 degree C

Western Blot (WB)

(Western blot analysis of Human, Rat, Mouse Hela cells, Brain, Lung showing detection of ~18 kDa SOD1 (UBB) protein using Rabbit Anti-SOD1 (UBB) Polyclonal Antibody (SPC-205). Lane 1: Molecular Weight Ladder. Lane 2: Human HeLa Cell Lysates. Lane 3: Rat Brain. Lane 4: Mouse Lung. Load: 20 ug. Primary Antibody: Rabbit Anti-SOD1 (UBB) Polyclonal Antibody (SPC-205) at 1:1000. Predicted/Observed Size: ~18 kDa.)

Related Product Information for anti-SOD1 antibody

Background Info: Recognizes a conformation specific epitope where the beta barrel is unfolded

Scientific Background: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (2). It works by catalyzing the dismutation of the superoxide radical O2" to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (1,4). In general, SODs play a major role in antioxidant defense mechanisms (3). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (2). Misfolding of SOD1 has been implicated in Amyotrophic lateral sclerosis (ALS). Therefore conformation specific antibodies such as Anti-SOD1 (UbetaB), which targets an unfolded region of the beta barrel of SOD1, are useful for determining the conformation of SOD1 in affected tissues (5). This antibody can be used in conjunction with Anti-SOD1 (EDI) (SPC-206D) which detects an exposed dimer interface (EDI) of SOD1.

Product Categories/Family for anti-SOD1 antibody

Oxidative Stress

References

1. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180. 2. Furukawa Y., and O'Halloran T. (2006) Antioxid Redox Signal. 8(5-6):847-67. 3. Gao B., et al. (2003) Am J Physiol Lung Cell Mol Physiol. 284:L917-L925. 4. Hassan H.M. (1988) Free Radical Biol. Med. 5:377-385. 5. Kerman A., et al. (2010) Acta Neuropathol. 119:335-344.

NCBI and Uniprot Product Information

• NCBI GI #: 49456443
• NCBI GeneID: 6647
• UniProt Accession #: P00441; Q16669; Q16711; Q16838; Q16839; Q16840; Q6NR85; A6NHJ0; D3DSE4
• Molecular Weight: 15,936 Da
• NCBI Official Full Name: SOD1
• NCBI Official Synonym Full Names: superoxide dismutase 1, soluble
• NCBI Official Symbol: SOD1
• NCBI Official Synonym Symbols: ALS; SOD; ALS1; IPOA; hSod1; HEL-S-44; homodimer
• NCBI Protein Information: superoxide dismutase [Cu-Zn]; superoxide dismutase [Cu-Zn]; SOD, soluble; indophenoloxidase A; Cu/Zn superoxide dismutase; superoxide dismutase, cystolic; epididymis secretory protein Li 44
• UniProt Protein Name: Superoxide dismutase [Cu-Zn]
• Protein Family: Superoxide dismutase
• UniProt Gene Name: SOD1
• UniProt Synonym Gene Names: hSod1
• UniProt Entry Name: SODC_HUMAN

NCBI Description

The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SOD1: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. Homodimer; non-disulfide linked. Homodimerization may take place via the ditryptophan cross-link at Trp-33. The pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 interact with RNF19A, whereas wild-type protein does not. The pathogenic variants ALS1 Arg-86 and Ala-94 interact with MARCH5, whereas wild-type protein does not. Belongs to the Cu-Zn superoxide dismutase family.

Protein type: Nuclear receptor co-regulator; Oxidoreductase; EC 1.15.1.1; Mitochondrial; Apoptosis

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: dendrite cytoplasm; extracellular space; protein complex; mitochondrion; extracellular region; mitochondrial intermembrane space; cytosol; nucleoplasm; extracellular matrix; cell soma; mitochondrial matrix; cytoplasm; plasma membrane; peroxisome; cytoplasmic vesicle; nucleus

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; copper ion binding; zinc ion binding; chaperone binding; superoxide dismutase activity; Rac GTPase binding; protein phosphatase 2B binding

Biological Process: positive regulation of catalytic activity; positive regulation of apoptosis; activation of MAPK activity; cellular iron ion homeostasis; myeloid cell homeostasis; retrograde axon cargo transport; response to antibiotic; muscle maintenance; retinal homeostasis; glutathione metabolic process; regulation of mitochondrial membrane potential; neurofilament cytoskeleton organization and biogenesis; positive regulation of superoxide release; negative regulation of neuron apoptosis; placenta development; response to drug; positive regulation of cytokine production; platelet activation; cell aging; transmission of nerve impulse; regulation of organ growth; response to reactive oxygen species; response to ethanol; response to heat; heart contraction; superoxide release; relaxation of vascular smooth muscle; removal of superoxide radicals; locomotory behavior; response to organic substance; sensory perception of sound; platelet degranulation; ovarian follicle development; regulation of blood pressure; auditory receptor cell stereocilium organization and biogenesis; response to axon injury; negative regulation of cholesterol biosynthetic process; anterograde axon cargo transport; response to nutrient levels; response to superoxide; thymus development; regulation of T cell differentiation in the thymus; response to amphetamine; superoxide metabolic process; myelin maintenance in the peripheral nervous system; regulation of multicellular organism growth; response to hydrogen peroxide; response to copper ion; spermatogenesis; regulation of protein kinase activity; blood coagulation; hydrogen peroxide biosynthetic process; embryo implantation

Disease: Amyotrophic Lateral Sclerosis 1