Rabbit anti-Human SNX4 Polyclonal Antibody | anti-SNX4 antibody

SNX4, CT (SNX4, Sorting nexin-4) (Azide free) (HRP)

Cat. Num. AAA6349698

• Gene Names: SNX4; ATG24B
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SNX4; Polyclonal Antibody; CT (SNX4; Sorting nexin-4) (Azide free) (HRP); Sorting nexin-4; anti-SNX4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SNX4 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SNX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 398-428 amino acids from the C-terminal region of human SNX4.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SNX4 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the SNX4 antibody detected the SNX4 protein (arrow).)

Immunohistochemistry (IHC)

(SNX4 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SNX4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-SNX4 antibody

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein associated with the long isoform of the leptin receptor and with receptor tyrosine kinases for platelet-derived growth factor, insulin, and epidermal growth factor in cell cultures, but its function is unknown. This protein may form oligomeric complexes with family members. [provided by RefSeq].

Product Categories/Family for anti-SNX4 antibody

Antibodies; SNX (Sorting Nexin) Proteins

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Skanland, S.S., et al. PLoS ONE 4 (6), E5935 (2009): Traer, C.J., et al. Nat. Cell Biol. 9(12):1370-1380(2007). Skanland, S.S., et al. Traffic 8(3):297-309(2007). Leprince, C., et al. J. Cell. Sci. 116 (PT 10), 1937-1948 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4507145
• NCBI GeneID: 8723
• NCBI Accession #: NP_003785.1
• NCBI GenBank Nucleotide #: NM_003794.3
• UniProt Accession #: O95219; B3KMH0; D3DNA3
• Molecular Weight: 51,909 Da
• NCBI Official Full Name: sorting nexin-4
• NCBI Official Synonym Full Names: sorting nexin 4
• NCBI Official Symbol: SNX4
• NCBI Official Synonym Symbols: ATG24B
• NCBI Protein Information: sorting nexin-4
• UniProt Protein Name: Sorting nexin-4
• Protein Family: Sorting nexin
• UniProt Gene Name: SNX4
• UniProt Entry Name: SNX4_HUMAN

NCBI Description

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein associated with the long isoform of the leptin receptor and with receptor tyrosine kinases for platelet-derived growth factor, insulin, and epidermal growth factor in cell cultures, but its function is unknown. This protein may form oligomeric complexes with family members. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene. [provided by RefSeq, Nov 2012]

Uniprot Description

SNX4: May be involved in several stages of intracellular trafficking. Plays a role in recycling endocytosed transferrin receptor and prevent its degradation. Belongs to the sorting nexin family.

Chromosomal Location of Human Ortholog: 3q21.2

Cellular Component: SNARE complex; protein complex; extrinsic to membrane; membrane; cytoplasmic dynein complex; early endosome membrane; cytoplasm; plasma membrane

Molecular Function: protein binding; insulin receptor binding; phosphoinositide binding; epidermal growth factor receptor binding

Biological Process: protein transport; vesicle organization and biogenesis; endocytic recycling