Western Blot (WB)
(All lanes : Anti-SNRPD1 Antibody (Center) at 1:1000 dilution Lane 1: HL-60 whole celll ysate Lane 2: K562 whole cell lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution. Predicted band size : 13 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit SNRPD1 Polyclonal Antibody | anti-SNRPD1 antibody
SNRPD1 Antibody (Center)
Predicted Reactivity: Bovine, Monkey, Mouse
Predicted Reactivity: Bovine, Monkey, Mouse
IHC-P~~1:50~100
FC~~1:10~50
,PubMed:28502770,PubMed:28781166,PubMed:28076346).Component of both the pre-catalyticspliceosome B complex and activatedspliceosome C complexes (PubMed:11991638,PubMed:26912367,PubMed:28502770,PubMed:28781166,PubMed:28076346). Is also acomponent of the minor U12 spliceosome(PubMed:15146077). May actas a charged protein scaffold to promotesnRNP assembly or strengthensnRNP-snRNP interactions throughnon-specific electrostatic contacts with RNA(Probable).
Western Blot (WB)
(All lanes : Anti-SNRPD1 Antibody (Center) at 1:1000 dilution Lane 1: HL-60 whole celll ysate Lane 2: K562 whole cell lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution. Predicted band size : 13 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-SNRPD1 Antibody (Center) at 1:1000 dilution Lane 1: HL-60 whole celll ysate Lane 2: K562 whole cell lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution. Predicted band size : 13 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with SNRPD1 Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with SNRPD1 Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(SNRPD1 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(SNRPD1 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Will,C.L., RNA 10 (6), 929-941 (2004)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a small nuclear ribonucleoprotein that belongs to the SNRNP core protein family. The protein may act as a charged protein scaffold to promote SNRNP assembly or strengthen SNRNP-SNRNP interactions through nonspecific electrostatic contacts with RNA. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]
Uniprot Description
snRNP D1: May act as a charged protein scaffold to promote snRNP assembly or strengthen snRNP-snRNP interactions through nonspecific electrostatic contacts with RNA. Belongs to the snRNP core protein family.
Protein type: RNA-binding; Spliceosome; RNA splicing
Chromosomal Location of Human Ortholog: 18q11.2
Cellular Component: small nuclear ribonucleoprotein complex; nucleoplasm; snRNP U1; nucleus; cytosol; U12-dependent spliceosome
Molecular Function: protein binding; RNA binding
Biological Process: nuclear mRNA splicing, via spliceosome; spliceosomal snRNP biogenesis; RNA splicing; spliceosome assembly; gene expression
Research Articles on SNRPD1
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Product Notes
The SNRPD1 snrpd1 (Catalog #AAA9201530) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 46-78. The SNRPD1 Antibody (Center) reacts with Human Predicted Reactivity: Bovine, Monkey, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SNRPD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS). WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50. Researchers should empirically determine the suitability of the SNRPD1 snrpd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SNRPD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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