Western Blot (WB)
(Biotinylated (0.1ug/ml) staining of A431 lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Goat Snail homolog 1 / SNAI1 Polyclonal Antibody | anti-SNAI1 antibody
Goat Anti-Snail homolog 1 / SNAI1, Biotinylated Antibody
Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat
Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat
Peptide ELISA: Antibody detection limit dilution 1:128000.
Western Blot (WB)
(Biotinylated (0.1ug/ml) staining of A431 lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Western Blot (WB)
(Biotinylated (0.1ug/ml) staining of A431 lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
NCBI and Uniprot Product Information
NCBI Description
The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq, Jul 2008]
Uniprot Description
Snail1: a protein of the C2H2-type zinc-finger family that regulates transcription. Involved in embryonic mesoderm formation. Plays a role in the epithelial to mesenchymal transition (EMT). EMT is characterized by decreased levels of the epithelial markers E-cadherin and alpha- and beta-catenin, and increased cellular migration and expression of the mesenchymal markers fibronectin, vimentin, N-cadherin and smooth muscle alpha-actin.E Snail binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. NF-kappaB binds and regulates the snail promoter and is critical for EMT, and inhibition of NF-kappaB activity lowered snail levels and morphologically reversed the EMT.
Protein type: C2H2-type zinc finger protein; Transcription factor
Chromosomal Location of Human Ortholog: 20q13.2
Cellular Component: cytoplasm; nucleus
Molecular Function: kinase binding; protein binding
Biological Process: epithelial to mesenchymal transition; mesoderm formation; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of transcription from RNA polymerase II promoter; osteoblast differentiation; positive regulation of cell migration; positive regulation of transcription, DNA-dependent
Research Articles on SNAI1
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Product Notes
The SNAI1 snai1 (Catalog #AAA423683) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-Snail homolog 1 / SNAI1, Biotinylated Antibody reacts with Human, Mouse, Rat Tested: Human, Mouse Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Snail homolog 1 / SNAI1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Western Blot: Approx 28kDa band observed in lysates of cell line A431 (calculated MW of 29.1kDa according to NP_005976.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.03-0.1ug/ml. Peptide ELISA: Antibody detection limit dilution 1:128000. Researchers should empirically determine the suitability of the SNAI1 snai1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RKPSDPNRKP NY. It is sometimes possible for the material contained within the vial of "Snail homolog 1 / SNAI1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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