Western Blot (WB)
(Staining (0.5ug/ml) of Molt-4 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat SMUG1 Polyclonal Antibody | anti-SMUG1 antibody
Goat anti-SMUG1 Antibody
Western Blot: Approx 35kDa band observed in Molt-4 lysates (predicted MW of 31kDa according to NP_055126. Recommended for use at 0.5-2ug/ml.
Western Blot (WB)
(Staining (0.5ug/ml) of Molt-4 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (0.5ug/ml) of Molt-4 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that participates in base excision repair by removing uracil from single- and double-stranded DNA. Many alternatively spliced transcript variants exist for this gene; the full-length nature is known for some but not all of the variants. [provided by RefSeq, Aug 2011]
Uniprot Description
SMUG1: Responsible for recognizing base lesions in the genome and initiating base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA and has a preference for single-stranded DNA substrates. The activity is greater against mismatches (U/G) than against matches (U/A). Excised uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5- hydroxymethyluracil (hmU)] in ssDNA and dsDNA but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine) and other oxidized damage. The activity is damage specificity and salt concentration-dependent. The general order of the preference for ssDNA and dsDNA is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at the low salt concentration. At the high concentration dsDNA (G pair) > dsDNA (A pair) > ssDNA. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Hydrolase; EC 3.2.2.-; DNA repair, damage
Chromosomal Location of Human Ortholog: 12q13.13
Cellular Component: nucleolus; nucleoplasm
Molecular Function: DNA binding; DNA N-glycosylase activity; oxidized pyrimidine base lesion DNA N-glycosylase activity; protein binding; single-strand selective uracil DNA N-glycosylase activity; uracil DNA N-glycosylase activity
Biological Process: base-excision repair; depyrimidination
Research Articles on SMUG1
Similar Products
Product Notes
The SMUG1 smug1 (Catalog #AAA421412) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-SMUG1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's SMUG1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 35kDa band observed in Molt-4 lysates (predicted MW of 31kDa according to NP_055126. Recommended for use at 0.5-2ug/ml. Researchers should empirically determine the suitability of the SMUG1 smug1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PQAFLLGSIH EPA. It is sometimes possible for the material contained within the vial of "SMUG1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
