Rabbit anti-Human SMPD1 Polyclonal Antibody | anti-SMPD1 antibody

SMPD1, CT (SMPD1, ASM, Sphingomyelin phosphodiesterase, Acid sphingomyelinase) (AP)

Cat. Num. AAA6349023

• Gene Names: SMPD1; ASM; NPD; ASMASE
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: SMPD1; Polyclonal Antibody; CT (SMPD1; ASM; Sphingomyelin phosphodiesterase; Acid sphingomyelinase) (AP); Acid sphingomyelinase; anti-SMPD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SMPD1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-SMPD1 antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 391-419 in the C-terminal region of human SMPD1. Species sequence homology: bovine
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of K562 cell line lysates (35ug/lane) usingMBS641963. This demonstrates MBS641963 detected the SMPD1 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of MBS641963 in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS641963 for immunohistochemistry.)

Testing Data

(All lanes : MBS641963 (1:4000) Lane 1: K562 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: A431 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 70 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Product Categories/Family for anti-SMPD1 antibody

Antibodies; Enzymes; Phosphodiesterase

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Desnick, J.P., et al. Mol. Med. 16 (7-8), 316-321 (2010): Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Sleat, D.E., et al. Mol. Cell Proteomics 5(4):686-701(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 56117840
• NCBI GeneID: 6609
• NCBI Accession #: NP_000534.3
• NCBI GenBank Nucleotide #: NM_000543.4
• UniProt Accession #: P17405; P17406; Q13811; Q16837; Q16841; A8K8M3; E9PKS3
• Molecular Weight: 629
• NCBI Official Full Name: sphingomyelin phosphodiesterase isoform 1
• NCBI Official Synonym Full Names: sphingomyelin phosphodiesterase 1, acid lysosomal
• NCBI Official Symbol: SMPD1
• NCBI Official Synonym Symbols: ASM; NPD; ASMASE
• NCBI Protein Information: sphingomyelin phosphodiesterase; acid sphingomyelinase
• UniProt Protein Name: Sphingomyelin phosphodiesterase
• Protein Family: Sphingomyelin phosphodiesterase
• UniProt Gene Name: SMPD1
• UniProt Synonym Gene Names: ASM; aSMase
• UniProt Entry Name: ASM_HUMAN

Uniprot Description

SMPD1: Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity. Monomer. Belongs to the acid sphingomyelinase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - sphingolipid; EC 3.1.4.12; Phosphodiesterase

Chromosomal Location of Human Ortholog: 11p15.4-p15.1

Cellular Component: extracellular space; lysosomal lumen; lysosome; plasma membrane; endosome; lamellar body

Molecular Function: protein binding; sphingomyelin phosphodiesterase activity; hydrolase activity, acting on glycosyl bonds

Biological Process: response to drug; negative regulation of MAP kinase activity; nervous system development; sphingomyelin metabolic process; sphingolipid metabolic process; positive regulation of apoptosis; ceramide biosynthetic process; glycosphingolipid metabolic process; sphingomyelin catabolic process; response to cocaine; signal transduction; positive regulation of protein amino acid dephosphorylation

Disease: Niemann-pick Disease, Type B; Niemann-pick Disease, Type A