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Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SMO antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s)
Rabbit SMO Polyclonal Antibody | anti-SMO antibody
smo
Gene Names
SMO; Gx; SMOH; FZD11
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Synonyms
SMO; Polyclonal Antibody; smo; CRJS; FZD11; Gx; SMOH; smoothened homolog; anti-SMO antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Sequence Length
787
Applicable Applications for anti-SMO antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Recommended Dilutions:
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 554-787 of human SMO (NP_005622.1).
Species Reactivity/Cross-Reactivity
Human, Mouse, Rat
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SMO antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SMO antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SMO antibody (MBS129037) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SMO antibody (MBS129037) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s)
Western Blot (WB)
(Western blot analysis of extracts of Mouse brain, using SMO antibody (MBS129037) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s)
Western Blot (WB)
(Western blot analysis of extracts of Mouse brain, using SMO antibody (MBS129037) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat brain using SMO antibody (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat brain using SMO antibody (40x lens).)
Immunofluorescence (IF)
(Immunofluorescence analysis of C6 cells using SMO Polyclonal Antibody (MBS129037) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of C6 cells using SMO Polyclonal Antibody (MBS129037) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells using SMO Polyclonal Antibody (MBS129037) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells using SMO Polyclonal Antibody (MBS129037) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Related Product Information for anti-SMO antibody
The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed MW 86kDa
Calculated MW 86kDa
Calculated MW 86kDa
NCBI Official Full Name
smoothened homolog
NCBI Official Synonym Full Names
smoothened, frizzled family receptor
NCBI Official Symbol
SMO
NCBI Official Synonym Symbols
Gx; SMOH; FZD11
NCBI Protein Information
smoothened homolog; protein Gx; frizzled family member 11; seven transmembrane helix receptor; smoothened, seven transmembrane spanning receptor
UniProt Protein Name
Smoothened homolog
Protein Family
UniProt Gene Name
SMO
UniProt Synonym Gene Names
SMOH; SMO
UniProt Entry Name
SMO_HUMAN
Similar Products
Product Notes
The SMO smo (Catalog #AAA129037) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SMO can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Recommended Dilutions: WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100. Researchers should empirically determine the suitability of the SMO smo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMO, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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