Rabbit anti-Human SMCP Polyclonal Antibody | anti-SMCP antibody

SMCP, CT (SMCP, MCS, MCSP, Sperm mitochondrial-associated cysteine-rich protein) (Azide free) (HRP)

Cat. Num. AAA6348928

• Gene Names: SMCP; MCS; MCSP; HSMCSGEN1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SMCP; Polyclonal Antibody; CT (SMCP; MCS; MCSP; Sperm mitochondrial-associated cysteine-rich protein) (Azide free) (HRP); Sperm mitochondrial-associated cysteine-rich protein; anti-SMCP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SMCP antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SMCP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-105 amino acids from the C-terminal region of human SMCP.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SMCP Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the SMCP antibody detected the SMCP protein (arrow).)

Related Product Information for anti-SMCP antibody

Sperm mitochondria differ in morphology and subcellular localization from those of somatic cells. They are elongated, flattened, and arranged circumferentially to form a helical coiled sheath in the midpiece of the sperm flagellum. The protein encoded by this gene localizes to the capsule associated with the mitochondrial outer membranes and is thought to function in the organization and stabilization of the helical structure of the sperm's mitochondrial sheath.

Product Categories/Family for anti-SMCP antibody

Antibodies; Mitochondrial Proteins

References

Yatsenko, A.N., et al. Hum. Mol. Genet. 15(23):3411-3419(2006). Hawthorne, S.K., et al. Genomics 87(3):382-391(2006). Behne, D., et al. Annu. Rev. Nutr. 21, 453-473 (2001): Cataldo, L., et al. Mol. Reprod. Dev. 45(3):320-331(1996). Aho, H., et al. Genomics 32(2):184-190(1996).

NCBI and Uniprot Product Information

• NCBI GI #: 25121989
• NCBI GeneID: 4184
• NCBI Accession #: NP_109588.2
• NCBI GenBank Nucleotide #: NM_030663.2
• UniProt Accession #: P49901; Q96A42
• Molecular Weight: 12,767 Da
• NCBI Official Full Name: sperm mitochondrial-associated cysteine-rich protein
• NCBI Official Synonym Full Names: sperm mitochondria-associated cysteine-rich protein
• NCBI Official Symbol: SMCP
• NCBI Official Synonym Symbols: MCS; MCSP; HSMCSGEN1
• NCBI Protein Information: sperm mitochondrial-associated cysteine-rich protein; mitochondrial capsule selenoprotein
• UniProt Protein Name: Sperm mitochondrial-associated cysteine-rich protein
• Protein Family: Sperm mitochondrial-associated cysteine-rich protein
• UniProt Gene Name: SMCP
• UniProt Synonym Gene Names: MCS; MCSP
• UniProt Entry Name: MCSP_HUMAN

NCBI Description

Sperm mitochondria differ in morphology and subcellular localization from those of somatic cells. They are elongated, flattened, and arranged circumferentially to form a helical coiled sheath in the midpiece of the sperm flagellum. The protein encoded by this gene localizes to the capsule associated with the mitochondrial outer membranes and is thought to function in the organization and stabilization of the helical structure of the sperm's mitochondrial sheath. [provided by RefSeq, Jul 2008]

Uniprot Description

SMCP: Involved in sperm motility. Its absence is associated with genetic background dependent male infertility. Infertility may be due to reduced sperm motility in the female reproductive tract and inability to penetrate the oocyte zona pellucida.

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: mitochondrion; mitochondrial membrane; cytoplasm

Biological Process: sperm motility; penetration of zona pellucida