Rabbit SMC3 Polyclonal Antibody | anti-SMC3 antibody

SMC3 (Phospho-S1083) Polyclonal Antibody

Cat. Num. AAA3007876

• Gene Names: SMC3; BAM; BMH; HCAP; CDLS3; CSPG6; SMC3L1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SMC3; Polyclonal Antibody; SMC3 (Phospho-S1083) Polyclonal Antibody; Structural maintenance of chromosomes protein 3; SMC protein 3; SMC-3; Basement membrane-associated chondroitin proteoglycan; Bamacan; Chondroitin sulfate proteoglycan 6; Chromosome-associated polypeptide; hCAP; BAM; BMH; CSPG6; SMC3L1; anti-SMC3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-SMC3 (S1083) polyclonal antibody detects endogenous levels of SMC3 protein only when phosphorylated at Ser1083.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1217

• Applicable Applications for anti-SMC3 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human SMC3 around the phosphorylation site of Ser1083.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of p-SMC3 (S1083) polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysateLane2:sp2/0 whole cell lysate Lane3:PC12 whole cell lysate)

Related Product Information for anti-SMC3 antibody

The SMC (structural maintenance of chromosomes) family of proteins form heterodimeric complexes that modulate sister chromatid cohesion and chromosome condensation for mitosis. The two distinct classes of SMC protein complexes are comprised of SMC1 (also designated SB1.8) with SMC3 (also designated HCAP for human chromosome-associated protein and Bamacan for the secreted proteoglycan), and SMC2 (also designated hCAP-E) with SMC4 (also designated hCAP-C). The SMC1/SMC3 complex is required for metaphase progression in mitotic cells and functions independently of the SMC2/SMC4 complex during the cell cycle. SMC1 is ubiqitiously expressed in various human tissues, including thymus, testis, and colon. SMC3 is expressed as a nuclear protein in the colon, but can also occur as a secreted proteoglycan expressed in testis and brain. The secreted proteoglycan contains several glycosylation sites and is thought to play a role in basement membrane physiology.

NCBI and Uniprot Product Information

• NCBI GI #: 29337005
• NCBI GeneID: 9126
• UniProt Accession #: Q9UQE7
• Molecular Weight: ~ 141kDa
• NCBI Official Full Name: Structural maintenance of chromosomes protein 3
• NCBI Official Synonym Full Names: structural maintenance of chromosomes 3
• NCBI Official Symbol: SMC3
• NCBI Official Synonym Symbols: BAM; BMH; HCAP; CDLS3; CSPG6; SMC3L1
• NCBI Protein Information: structural maintenance of chromosomes protein 3
• UniProt Protein Name: Structural maintenance of chromosomes protein 3
• UniProt Gene Name: SMC3
• UniProt Synonym Gene Names: BAM; BMH; CSPG6; SMC3L1; SMC protein 3; SMC-3; Bamacan; hCAP
• UniProt Entry Name: SMC3_HUMAN

NCBI Description

This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein. [provided by RefSeq, Jul 2008]

Uniprot Description

SMC3: Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement. Interacts with MXI1, MXD3 and MXD4. Interacts with SYCP2. Found in a complex with SMC1A, CDCA5 and RAD21, PDS5A/APRIN and PDS5B/SCC-112. Forms a heterodimer with SMC1A or SMC1B in cohesin complexes. Cohesin complexes are composed of the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer attached via their hinge domain, RAD21 which link them, and one STAG protein (STAG1, STAG2 or STAG3), which interacts with RAD21. Also found in meiosis-specific cohesin complexes. Interacts with NUMA1, and forms a ternary complex with KIF3B and KIFAP3, suggesting a function in tethering the chromosomes to the spindle pole and in chromosome movement. Interacts with PDS5A and WAPAL; regulated by SMC3 acetylation. Belongs to the SMC family. SMC3 subfamily.

Protein type: Cell cycle regulation; DNA repair, damage

Chromosomal Location of Human Ortholog: 10q25

Cellular Component: cohesin complex; spindle pole; nuclear matrix; cohesin core heterodimer; chromosome; cytosol; nucleoplasm; lateral element; cytoplasm; basement membrane; chromatin; nucleus; chromosome, pericentric region

Molecular Function: dynein binding; protein binding; protein heterodimerization activity; microtubule motor activity; chromatin binding; ATP binding

Biological Process: mitosis; meiosis; mitotic spindle organization and biogenesis; cell division; mitotic sister chromatid cohesion; stem cell maintenance; sister chromatid cohesion; negative regulation of DNA endoreduplication; mitotic cell cycle; signal transduction; DNA repair; regulation of DNA replication

Disease: Cornelia De Lange Syndrome 3