Rabbit anti-Human SMC1A Polyclonal Antibody | anti-SMC1A antibody

SMC1A, NT (SMC1A, DXS423E, KIAA0178, SB1.8, SMC1, SMC1L1, Structural maintenance of chromosomes protein 1A, Sb1.8) (PE)

Cat. Num. AAA6348901

• Gene Names: SMC1A; SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; SMC1alpha
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SMC1A; Polyclonal Antibody; NT (SMC1A; DXS423E; KIAA0178; SB1.8; SMC1; SMC1L1; Structural maintenance of chromosomes protein 1A; Sb1.8) (PE); Sb1.8; anti-SMC1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-SMC1A antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SMC1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 306-336 amino acids from the N-terminal region of human SMC1A.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SMC1A Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the SMC1A antibody detected the SMC1A protein (arrow).)

Related Product Information for anti-SMC1A antibody

Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1L2 or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. [provided by RefSeq].

Product Categories/Family for anti-SMC1A antibody

Antibodies; KIAA; orf

References

Limongelli, G., et al. Am. J. Med. Genet. A 152A (8), 2127-2129 (2010): Homme, C., et al. Oncol. Rep. 24(1):47-56(2010). Pie, J., et al. Am. J. Med. Genet. A 152A (4), 924-929 (2010): Wong, R.W. Cell Cycle 9(1):198-200(2010). Liu, J., et al. Hum. Mutat. 30(11):1535-1542(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 30581135
• NCBI GeneID: 8243
• NCBI Accession #: NP_006297.2
• NCBI GenBank Nucleotide #: NM_006306.2
• UniProt Accession #: Q14683; O14995; Q16351; Q2M228
• Molecular Weight: 143,233 Da
• NCBI Official Full Name: structural maintenance of chromosomes protein 1A
• NCBI Official Synonym Full Names: structural maintenance of chromosomes 1A
• NCBI Official Symbol: SMC1A
• NCBI Official Synonym Symbols: SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; SMC1alpha
• NCBI Protein Information: structural maintenance of chromosomes protein 1A; SMC-1A; SMC-1-alpha; SMC protein 1A; segregation of mitotic chromosomes 1; SMC1 structural maintenance of chromosomes 1-like 1; SMC1 (structural maintenance of chromosomes 1, yeast)-like 1
• UniProt Protein Name: Structural maintenance of chromosomes protein 1A
• Protein Family: Structural maintenance of chromosomes protein
• UniProt Gene Name: SMC1A
• UniProt Synonym Gene Names: DXS423E; KIAA0178; SB1.8; SMC1; SMC1L1; SMC protein 1A; SMC-1-alpha; SMC-1A
• UniProt Entry Name: SMC1A_HUMAN

NCBI Description

Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1L2 or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. [provided by RefSeq, Jul 2008]

Uniprot Description

Smc1: a component of the cohesin multiprotein complex that is required for sister chromatid cohesion. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Thought to be an important part of functional kinetochores. Interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair.

Protein type: RNA splicing; DNA replication

Chromosomal Location of Human Ortholog: Xp11.22-p11.21

Cellular Component: kinetochore; nucleoplasm; cohesin core heterodimer; condensed nuclear chromosome; cytoplasm; nucleolus; chromosome; nucleus; cytosol; chromosome, pericentric region

Molecular Function: protein binding; protein heterodimerization activity; microtubule motor activity; chromatin binding; ATP binding

Biological Process: meiosis; mitotic sister chromatid segregation; mitotic cell cycle checkpoint; RNA splicing; stem cell maintenance; mitotic sister chromatid cohesion; DNA damage response, signal transduction; sister chromatid cohesion; negative regulation of DNA endoreduplication; DNA repair; nuclear mRNA splicing, via spliceosome; response to radiation; mitotic spindle organization and biogenesis; cell division; gene expression; mitotic cell cycle

Disease: Cornelia De Lange Syndrome 2