Western Blot (WB)
(SMAGP Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane). This demonstrates the SMAGP antibody detected the SMAGP protein (arrow).)
Rabbit anti-Human SMAGP Polyclonal Antibody | anti-SMAGP antibody
SMAGP, ID (SMAGP, Small cell adhesion glycoprotein, Small transmembrane and glycosylated protein) (Biotin)
Gene Names
SMAGP; hSMAGP; MGC149453; MGC149454
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
SMAGP; Polyclonal Antibody; ID (SMAGP; Small cell adhesion glycoprotein; Small transmembrane and glycosylated protein) (Biotin); Small transmembrane and glycosylated protein; anti-SMAGP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-SMAGP antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
ELISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
SMAGP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-84 amino acids from the Central region of human SMAGP.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(SMAGP Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane). This demonstrates the SMAGP antibody detected the SMAGP protein (arrow).)
Western Blot (WB)
(SMAGP Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane). This demonstrates the SMAGP antibody detected the SMAGP protein (arrow).)
Immunohistochemistry (IHC)
(SMAGP Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SMAGP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(SMAGP Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SMAGP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(SMAGP Antibody (Center) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(SMAGP Antibody (Center) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-SMAGP antibody
SMAGP may play a role in epithelial cell-cell contacts. SMAGP may play a role in tumor invasiveness and metastasis formation.
Product Categories/Family for anti-SMAGP antibody
References
Tarbe, N.G., et al. Int. J. Cancer 117(6):913-922(2005). Tarbe, N.G., et al. Oncogene 23(19):3395-3403(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10,679 Da
NCBI Official Full Name
small cell adhesion glycoprotein
NCBI Official Synonym Full Names
small cell adhesion glycoprotein
NCBI Official Symbol
SMAGP
NCBI Official Synonym Symbols
hSMAGP; MGC149453; MGC149454
NCBI Protein Information
small cell adhesion glycoprotein; small transmembrane and glycosylated protein; small trans-membrane and glycosylated protein; small cell transmembrane and glycosylated protein
UniProt Protein Name
Small cell adhesion glycoprotein
Protein Family
UniProt Gene Name
SMAGP
UniProt Entry Name
SMAGP_HUMAN
Uniprot Description
SMAGP: May play a role in epithelial cell-cell contacts. May play a role in tumor invasiveness and metastasis formation. Belongs to the SMAGP family.
Protein type: Membrane protein, integral; Vesicle
Chromosomal Location of Human Ortholog: 12q13.13
Cellular Component: nucleoplasm; cytoplasmic vesicle membrane; plasma membrane; integral to membrane; cell junction
Similar Products
Product Notes
The SMAGP smagp (Catalog #AAA6348816) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SMAGP, ID (SMAGP, Small cell adhesion glycoprotein, Small transmembrane and glycosylated protein) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SMAGP can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 ELISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SMAGP smagp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMAGP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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